Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.
AuthorsSugimoto M,Fujikawa A,Womack JE,Sugimoto Y
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID16611727
Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal ... More
Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.
Rapamycin has been shown to affect translation. We have utilized two complementary approaches to identify genes that are predominantly affected by rapamycin in Jurkat T cells. One was to compare levels of polysome-bound and total RNA using oligonucleotide microarrays complementary to 6,300 human genes. Another was to determine protein synthesis ... More
Translational regulation of the JunD messenger RNA.
AuthorsShort John D; Pfarr Curt M;
JournalJ Biol Chem
PubMed ID12105216
JunD, a member of the Jun family of nuclear transcription proteins, dimerizes with Fos family members or other Jun proteins (c-Jun or JunB) to form the activator protein 1 (AP-1) transcription factor. The junD gene contains no introns and generates a single mRNA. Here we show that two predominant JunD ... More
A novel single amino acid deletion caspase-8 mutant in cancer cells that lost proapoptotic activity.
AuthorsLiu B, Peng D, Lu Y, Jin W, Fan Z.
JournalJ Biol Chem
PubMed ID12055196
'Caspase-8 is an important initiation caspase that activates the caspase cascade during death receptor-mediated apoptosis. We here report a novel caspase-8 mutant with a naturally occurring deletion of leucine 62 (Delta Leu62casp-8). Delta Leu62casp-8 has a shorter half-life than its wild-type counterpart. Unlike wild-type caspase-8, Delta Leu62casp-8 failed to interact ... More
Calpain-mediated X-linked inhibitor of apoptosis degradation in neutrophil apoptosis and its impairment in chronic neutrophilic leukemia.
'The number of neutrophils in the blood and tissues is controlled by constitutive apoptotic programmed cell death and clearance by phagocytes such as macrophages. Here, we found that calpains cleave the X-linked inhibitor of apoptosis (XIAP) in vitro, producing fragments that are unable to inhibit caspase-3. These fragments were detected ... More
A Novel Pathway for Nickel-induced Interleukin-8 Expression.
'Inhalation of particulate nickel subsulfide (Ni(3)S(2)) causes chronic active inflammation and fibrosis of the lungs. However, the mechanisms for these effects are not well understood. Therefore, cell culture experiments with BEAS-2B human airway epithelial cells were conducted to test the hypothesis that exposure to non-cytotoxic levels of Ni(3)S(2) induces expression ... More
STARS, a Striated Muscle Activator of Rho Signaling and Serum Response Factor-dependent Transcription.
Authors Arai Akiko; Spencer Jeffrey A; Olson Eric N;
JournalJ Biol Chem
PubMed ID11983702
'Changes in actin dynamics influence diverse cellular processes and couple the actin-based cytoskeleton to changes in gene transcription. Members of the Rho GTPase family regulate cytoskeletal organization by stimulating actin polymerization and stress fiber formation when activated by extracellular signaling. The transcriptional activity of serum response factor (SRF) is stimulated ... More
Authors Su Hua; Arakawa-Hoyt Janice; Kan Yuet Wai;
JournalProc Natl Acad Sci U S A
PubMed ID12084814
'Intramyocardial injection of genes encoding angiogenic factors could provide a useful approach for the treatment of ischemic heart disease. However, uncontrolled expression of angiogenic factors in vivo may cause some unwanted side effects, such as hemangioma formation, retinopathy, and arthritis. It may also induce occult tumor growth and artherosclerotic plaque ... More
Thiazolidinedione activation of peroxisome proliferator-activated receptor gamma can enhance mitochondrial potential and promote cell survival.
Authors Wang Y Lynn; Frauwirth Kenneth A; Rangwala Shamina M; Lazar Mitchell A; Thompson Craig B;
JournalJ Biol Chem
PubMed ID12082115
'Thiazolidinediones (TZDs) are widely used for treatment of type 2 diabetes mellitus. Peroxisome proliferator-activated receptor gamma (PPAR gamma) is the molecular target of TZDs and is believed to mediate the apoptotic effects of this class of drugs in a variety of cell types, including B and T lymphocytes. The finding ... More
Unsaturated fatty acids inhibit cholesterol efflux from macrophages by increasing degradation of ATP-binding cassette transporter A1.
Authors Wang Yutong; Oram John F;
JournalJ Biol Chem
PubMed ID11741998
'Abnormal high density lipoprotein metabolism may contribute to the increased atherosclerosis associated with diabetes and insulin resistance. The ATP-binding cassette transporter ABCA1 mediates cholesterol transport from tissue macrophages to apoA-I, the major high density lipoprotein protein component. Because fatty acids are elevated in diabetes, we examined the effects of fatty ... More
Reconstitution of human DNA polymerase delta using recombinant baculoviruses: the p12 subunit potentiates DNA polymerizing activity of the four-subunit enzyme.
Authors Podust Vladimir N; Chang Long-Sheng; Ott Robert; Dianov Grigory L; Fanning Ellen;
JournalJ Biol Chem
PubMed ID11711545
'Eukaryotic DNA polymerase delta is thought to consist of three (budding yeast) or four subunits (fission yeast, mammals). Four human genes encoding polypeptides p125, p50, p66, and p12 have been assigned as subunits of DNA polymerase delta. However, rigorous purification of human or bovine DNA polymerase delta from natural sources ... More
Repression of activator protein-1-mediated transcriptional activation by the Notch-1 intracellular domain.
Authors Chu Jianlin; Jeffries Shawn; Norton Jason E; Capobianco Anthony J; Bresnick Emery H;
JournalJ Biol Chem
PubMed ID11739397
'Developmental decisions that control cell fate are commonly regulated by the Notch signaling pathway. Activation of transmembrane Notch receptors results in proteolytic liberation of the intracellular domain of Notch, which translocates into the nucleus, binds a repressor (C promoter binding factor 1/RBP-Jkappa, Su(H), and Lag-1 (CSL)), and induces target genes. ... More
Lack of the small plastid-encoded PsbJ polypeptide results in a defective water-splitting apparatus of photosystem II, reduced photosystem I levels, and hypersensitivity to light.
'Photosystem II is a large pigment-protein complex catalyzing water oxidation and initiating electron transfer processes across the thylakoid membrane. In addition to large protein subunits, many of which bind redox cofactors, photosystem II particles contain a number of low molecular weight polypeptides whose function is only poorly defined. Here we ... More
Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution.
'A cDNA from Arabidopsis thaliana and four related cDNAs from Nicotiana tabacum that we have isolated encode hitherto unidentified members of the mitochondrial carrier family. These proteins have been overexpressed in bacteria and reconstituted into phospholipid vesicles. Their transport properties demonstrate that they are orthologs/isoforms of a novel mitochondrial carrier ... More
The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnover.
Authors Toompuu Marina; Yasukawa Takehiro; Suzuki Tsutomu; Hakkinen Terhi; Spelbrink Johannes N; Watanabe Kimitsuna; Jacobs Howard T;
JournalJ Biol Chem
PubMed ID11919191
'The 7472insC mitochondrial DNA mutation in the tRNA(Ser(UCN)) gene is associated with sensorineural deafness combined, in some patients, with a wider neurological syndrome. In cultured cybrid cells it causes a 70% decrease in tRNA(Ser(UCN)) abundance and mild respiratory impairment, previously suggested to be due to decreased tRNA stability. When mitochondrial ... More
Norepinephrine increases I kappa B alpha expression in astrocytes.
'The neurotransmitter norepinephrine (NE) can inhibit inflammatory gene expression in glial cells; however, the mechanisms involved are not clear. In primary astrocytes, NE dose-dependently increased the expression of inhibitory I kappa B alpha protein accompanied by an increase in steady state levels of I kappa B alpha mRNA. Maximal increases ... More
Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes.
Authors Paquin Joanne; Danalache Bogdan A; Jankowski Marek; McCann Samuel M; Gutkowska Jolanta;
JournalProc Natl Acad Sci U S A
PubMed ID12093924
'We recently discovered the existence of the oxytocin/oxytocin receptor (OT/OTR) system in the heart. Activation of cardiac OTR stimulates the release of atrial natriuretic peptide (ANP), which is involved in regulation of blood pressure and cell growth. Having observed elevated OT levels in the fetal and newborn heart at a ... More
Transcriptional effects of chronic Akt activation in the heart.
Authors Cook Stuart A; Matsui Takashi; Li Ling; Rosenzweig Anthony;
JournalJ Biol Chem
PubMed ID11956204
'Akt activation reduces cardiomyocyte death and induces cardiac hypertrophy. To help identify effector mechanisms, gene expression profiles in hearts from transgenic mice with cardiac-specific expression of activated Akt (myr-Akt) were compared with littermate controls. 40 genes were identified as differentially expressed. Quantitative reverse transcription-PCR confirmed qualitative results of transcript profiling ... More
The transcriptome profile of human embryonic stem cells as defined by SAGE.
AuthorsRichards M, Tan SP, Tan JH, Chan WK, Bongso A,
JournalStem Cells
PubMed ID14688391
'Human embryonic stem (ES) cell lines that have the ability to self-renew and differentiate into specific cell types have been established. The molecular mechanisms for self-renewal and differentiation, however, are poorly understood. We determined the transcriptome profiles for two proprietary human ES cell lines (HES3 and HES4, ES Cell International), ... More
The identification and functional characterization of a novel mast cell isoform of the microphthalmia-associated transcription factor.
AuthorsTakemoto CM, Yoon YJ, Fisher DE.
JournalJ Biol Chem
PubMed ID12039954
'The microphthalmia-associated transcription factor (Mitf) is critical for mast cell development based on the severe mast cell deficiency seen in Mitf mutant mice. Mitf also is important for the development of melanocytes, osteoclasts, and retinal pigment epithelium. The lineage-restricted phenotypes of Mitf mutations correlate with tissue-restricted expression of Mitf, a ... More
Overexpression of Phex in osteoblasts fails to rescue the Hyp mouse phenotype.
Authors Liu Shiguang; Guo Rong; Tu Qisheng; Quarles L Darryl;
JournalJ Biol Chem
PubMed ID11713245
'Inactivating mutations of Phex, a phosphate-regulating endopeptidase, cause hypophosphatemia and impaired mineralization in X-linked hypophosphatemia (XLH) and its mouse homologue, Hyp. Because Phex is predominantly expressed in bone and cultured osteoblasts from Hyp mice display an apparent intrinsic mineralization defect, it is thought that reduced expression of Phex in mature ... More
Activation of macrophages by linear (1right-arrow3)-beta-D-glucans. Impliations for the recognition of fungi by innate immunity.
'Although (1-->3)-beta-d-glucans, which are one of major fungal cell wall components, are known to activate invertebrate innate immune systems, their activities on mammalian cells remain elusive. Here, we report their activities on mouse macrophages. Among the various (1-->3)-beta-d-glucans, curdlan, a linear (1-->3)-beta-d-glucan, although not branched beta-glucans, exhibits significant activity to ... More
Acetylcholine-induced phosphatidylinositol 4,5-bisphosphate depletion does not cause short-term desensitization of G protein-gated inwardly rectifying K+ current in mouse atrial myocytes.
Authors Cho Hana; Hwang Ji-Young; Kim Daesoo; Shin Hee-Sup; Kim Yangmi; Earm Yung E; Ho Won-Kyung;
JournalJ Biol Chem
PubMed ID12019267
'Depletion of phosphatidylinositol 4,5-bisphosphate (PIP(2)) induced by phenylephrine or endothelin causes the inhibition of acetylcholine-activated K(+) current (I(KACh)) in atrial myocytes. In the present study, we have investigated the hypothesis that muscarinic receptor induced PIP(2) depletion also causes inhibition of I(KACh), resulting in desensitization. We confirmed the expression of G(q)-coupled ... More
Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in cultured cells and functional aspects.
'Deoxynucleoside triphosphates (dNTPs) used for mitochondrial DNA replication are mainly formed by phosphorylation of deoxynucleosides imported into mitochondria from the cytosol. We earlier obtained evidence for a mitochondrial 5''-nucleotidase (dNT2) with a pronounced specificity for dUMP and dTMP and suggested that the enzyme protects mitochondrial DNA replication from excess dTTP. ... More
Liver X receptors as insulin-mediating factors in fatty acid and cholesterol biosynthesis.
Authors Tobin Kari Anne Risan; Ulven Stine M; Schuster Gertrud U; Steineger Hilde Hermansen; Andresen Sissel Mahle; Gustafsson Jan-Ake; Nebb Hilde Irene;
JournalJ Biol Chem
PubMed ID11781314
'The nuclear receptor liver X receptor (LXR) alpha, an important regulator of cholesterol and bile acid metabolism, was analyzed after insulin stimulation in liver in vitro and in vivo. A time- and dose-dependent increase in LXRalpha steady-state mRNA level was seen after insulin stimulation of primary rat hepatocytes in culture. ... More
A Single Nucleotide Polymorphism in the Matrix Metalloproteinase-1 (MMP-1) Promoter Influences Amnion Cell MMP-1 Expression and Risk for Preterm Premature Rupture of the Fetal Membranes.
'Interstitial collagen gives fetal membranes tensile strength, and membrane rupture has been attributed to collagen degradation. A polymorphism at −1607 in the matrix metalloproteinase-1 (MMP-1) promoter (an insertion of a guanine (G)) creates a core Ets binding site and increases promoter activity. We investigated whether this polymorphism is functionally significant ... More
Reduction in intracellular calcium levels inhibits myoblast differentiation.
Authors Porter George A Jr; Makuck Ryan F; Rivkees Scott A;
JournalJ Biol Chem
PubMed ID12042317
'In myocytes, calcium plays an important role in intracellular signaling and contraction. However, the ability of calcium to modulate the differentiation of striated muscle cells is poorly understood. To examine this issue we studied C2C12 cells, which is a myoblast cell line that differentiates in vitro. First, we observed that ... More
Glucocorticoid Activation of Na+/H+ Exchanger Isoform 3 Revisited. THE ROLES OF SGK1 AND NHERF2.
Authors Yun C Chris; Chen Yueping; Lang Florian;
JournalJ Biol Chem
PubMed ID11751930
'The stimulative effect of glucocorticoids on intestinal salt and water absorption has been known for more than two decades. However, molecular mechanisms underlying this activation remain elusive. Previous studies showed that methylprednisolone specifically increased Na(+)/H(+) exchanger isoform (NHE) 3 mRNA in ileum and kidney without affecting NHE1 mRNA levels. These ... More
Regulation of a xenobiotic sulfonation cascade by nuclear pregnane X receptor (PXR).
Authors Sonoda Junichiro; Xie Wen; Rosenfeld John M; Barwick Joyce L; Guzelian Philip S; Evans Ronald M;
JournalProc Natl Acad Sci U S A
PubMed ID12370413
'The nuclear receptor PXR (pregnane X receptor) protects the body from hepatotoxicity of secondary bile acids such as lithocholic acid (LCA) by inducing expression of the hydroxylating cytochrome P450 enzyme CYP3A and promoting detoxification. We found that activation of PXR also increases the activity and gene expression of the phase ... More
Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants.
AuthorsWang D, Malo D, Hekimi S,
JournalJ Immunol
PubMed ID20007531
'Mitochondrial reactive oxygen species (ROS) are believed to stabilize hypoxia-inducible factor (HIF)-1alpha, a transcriptional regulator of the immune response. Mclk1 encodes a mitochondrial protein that is necessary for ubiquinone biosynthesis. Heterozygote Mclk1(+/-) mutant mice are long-lived despite increased mitochondrial ROS and decreased energy metabolism. In this study, Mclk1(+/-) mutant mice ... More
Molecular characterization of the homo-phytochelatin synthase of soybean Glycine max: relation to phytochelatin synthase.
Authors Oven Matjaz; Page Jonathan E; Zenk Meinhart H; Kutchan Toni M;
JournalJ Biol Chem
PubMed ID11706029
'The phytochelatin homologs homo-phytochelatins are heavy metal-binding peptides present in many legumes. To study the biosynthesis of these compounds, we have isolated and functionally expressed a cDNA GmhPCS1 encoding homo-phytochelatin synthase from Glycine max, a plant known to accumulate homo-phytochelatins rather than phytochelatins upon the exposure to heavy metals. The ... More
Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways.
'The androgen receptor (AR) is a ligand-activated transcription factor that mediates the biological responses of androgens. However, non-androgenic pathways have also been shown to activate the AR. The mechanism of cross-talk between the interleukin-6 (IL-6) and AR signal transduction pathways was investigated in LNCaP human prostate cancer cells. IL-6 induced ... More
Molecular mechanism of the induction of metalloproteinases 1 and 3 in human fibroblasts by basic calcium phosphate crystals. Role of calcium-dependent protein kinase C alpha.
Authors Reuben Paul M; Brogley Michele A; Sun Yubo; Cheung Herman S;
JournalJ Biol Chem
PubMed ID11836255
'Synovial fluid basic calcium phosphate (BCP) crystals are common in osteoarthritis and are often associated with destructive arthropathies involving cartilage degeneration. These crystals are mitogenic and induce oncogene expression and matrix metalloproteinase (MMP) synthesis and secretion in human fibroblasts. To date, BCP crystal-elicited signal transduction pathways have not been completely ... More
Insulin Stimulates Hypoxia-inducible Factor 1 through a Phosphatidylinositol 3-Kinase/Target of Rapamycin-dependent Signaling Pathway.
'Hypoxia-inducible factor 1 (HIF-1) is a transcription factor involved in normal mammalian development and in the pathogenesis of several disease states. It consists of two subunits, HIF-1alpha, which is degraded during normoxia, and HIF-1beta, which is constitutively expressed. Activated HIF-1 induces the expression of genes involved in angiogenesis, erythropoiesis, and ... More
Endothelin-1 Induces Vascular Endothelial Growth Factor by Increasing Hypoxia-inducible Factor-1alpha in Ovarian Carcinoma Cells.
'Angiogenesis is an essential prerequisite for tumor growth, invasion, and metastasis. In ovarian carcinoma cells, endothelin-1 (ET-1) stimulates the secretion of vascular endothelial growth factor (VEGF), a major mediator of tumor angiogenesis. In OVCA 433 and HEY ovarian carcinoma cell lines, ET-1 treatment increases VEGF mRNA expression and induces VEGF ... More
Hypomorphic apolipoprotein E mice: a new model of conditional gene repair to examine apolipoprotein E-mediated metabolism.
Authors Raffai Robert L; Weisgraber Karl H;
JournalJ Biol Chem
PubMed ID11792702
'In creating an allelic variant of mouse Apoe designed to resemble human apolipoprotein E4 (apoE4), we generated hypomorphic apoE (hypoE) mice that express only approximately 5% of normal apoE mRNA levels in all tissues. Insertion of a neo cassette flanked by loxP sites in the third intron of Apoe reduced ... More
Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: Implications for regulation and cellular function.
Authors Hubert Nadia; Hentze Matthias W;
JournalProc Natl Acad Sci U S A
PubMed ID12209011
'Divalent metal transporter 1 (DMT1) mediates apical iron uptake into duodenal enterocytes and also transfers iron from the endosome into the cytosol after cellular uptake via the transferrin receptor. Hence, mutations in DMT1 cause systemic iron deficiency and anemia. DMT1 mRNA levels are increased in the duodenum of iron-deficient animals. ... More
CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome.
Authors Wang Xiping; Kang Dingming; Feng Suhua; Serino Giovanna; Schwechheimer Claus; Wei Ning;
JournalMol Biol Cell
PubMed ID11854419
'The COP9 signalosome (CSN) is a multifunctional protein complex essential for arabidopsis development. One of its functions is to promote Rub1/Nedd8 deconjugation from the cullin subunit of the Skp1-cullin-F-box ubiquitin ligase. Little is known about the specific role of its eight subunits in deneddylation or any of the physiological functions ... More
WY14,643, a peroxisome proliferator-activated receptor alpha (PPARalpha ) agonist, improves hepatic and muscle steatosis and reverses insulin resistance in lipoatrophic A-ZIP/F-1 mice.
Authors Chou Chieh J; Haluzik Martin; Gregory Charmaine; Dietz Kelly R; Vinson Charles; Gavrilova Oksana; Reitman Marc L;
JournalJ Biol Chem
PubMed ID11994294
'WY14,643 is a specific peroxisome proliferator-activated receptor alpha (PPARalpha) agonist with strong hypolipidemic effects. Here we have examined the effect of WY14,643 in the A-ZIP/F-1 mouse, a model of severe lipoatrophic diabetes. With 1 week of treatment, all doses of WY14,643 that were tested normalized serum triglyceride and fatty acid ... More
Cloning and characterization of a lysosomal phospholipase A2, 1-o-acylceramide synthase.
Authors Hiraoka Miki; Abe Akira; Shayman James A;
JournalJ Biol Chem
PubMed ID11790796
'Recently, a novel enzyme, 1-O-acylceramide synthase (ACS), was purified and characterized from bovine brain. This enzyme has both calcium-independent phospholipase A(2) and transacylase activities. The discovery of this enzyme led us to propose a new pathway for ceramide metabolism in which the sn-2-acyl group of either phosphatidylethanolamine or phosphatidylcholine is ... More
Regulation of the cellular prion protein gene expression depends on chromatin conformation.
Authors Cabral Ana Lucia B; Lee Kil S; Martins Vilma R;
JournalJ Biol Chem
PubMed ID11739375
'Conversion of the normal cellular prion protein (PrPc), whose physiological function is still under investigation, to an infectious form called prion is the cause of some neurodegenerative diseases. Therefore, the elucidation of PrPc gene regulation is important both to define a strategy to control the infection and to better understand ... More
Ubiquitin/proteasome-dependent degradation of D-type cyclins is linked to tumor necrosis factor-induced cell cycle arrest.
Authors Hu Xiaotang; Bryington Matthew; Fisher Ariana B; Liang Xiaomei; Zhang Xiaohong; Cui Dongming; Datta Indrani; Zuckerman Kenneth S;
JournalJ Biol Chem
PubMed ID11864973
'Tumor necrosis factor-alpha (TNF) is well known for its cytotoxic effect on malignant cells. Its role in cell cycle control is relatively less known. In this study, we found that TNF induced G(1) arrest of TF-1 and MV4-11 cells while simultaneously causing apoptosis. Treatment of the cells with TNF for ... More
GC box-binding transcription factors control the neuronal specific transcription of the cyclin-dependent kinase 5 regulator p35.
Authors Ross Sarah; Tienhaara Anri; Lee Ming-Sum; Tsai Li-Huei; Gill Grace;
JournalJ Biol Chem
PubMed ID11724806
'Cyclin-dependent kinase 5 (cdk5)/p35 kinase activity is highest in post-mitotic neurons of the central nervous system and is critical for development and function of the brain. The neuronal specific activity of the cdk5/p35 kinase is achieved through the regulated expression of p35 mRNA. We have identified a small 200-bp fragment ... More
Megakaryoblastic leukemia factor-1 transduces cytoskeletal signals and induces smooth muscle cell differentiation from undifferentiated embryonic stem cells.
AuthorsDu KL, Chen M, Li J, Lepore JJ, Mericko P, Parmacek MS,
JournalJ Biol Chem
PubMed ID14970199
'The SAP domain transcription factor myocardin plays a critical role in the transcriptional program regulating smooth muscle cell differentiation. In this report, we describe the capacity of myocardin to physically associate with megakaryoblastic leukemia factor-1 (MKL1) and characterize the function of MKL1 in smooth muscle cells (SMCs). The MKL1 gene ... More
Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter.
Authors Korchynskyi Olexander; ten Dijke Peter;
JournalJ Biol Chem
PubMed ID11729207
'Transforming growth factor-beta (TGF-beta) family members, which include bone morphogenetic proteins (BMPs) and TGF-betas, elicit their cellular effects by activating specific Smad proteins, which control the transcription of target genes. BMPs and TGF-betas have overlapping as well as specific effects on mesenchymal cell differentiation for which the mechanisms are incompletely ... More
Activation of large conductance sodium channels upon expression of amiloride-sensitive sodium channel in Sf9 insect cells.
Authors Rao U Subrahmanyeswara; Steimle Randy E; Balachandran Premalatha;
JournalJ Biol Chem
PubMed ID11733494
'The amiloride-sensitive epithelial sodium channels (ENaC) mediate Na(+) reabsorption in epithelial tissues including distal nephron, colon, lung, and secretory glands and plays a critical role in pathophysiology of hypertension and cystic fibrosis. The ENaC is a multimeric protein composed of alpha-ENaC, beta-ENaC, and gamma-ENaC subunits. To study the biochemical properties ... More
Ghrelin modulates the downstream molecules of insulin signaling in hepatoma cells.
'Ghrelin was identified in the stomach as an endogenous ligand specific for the growth hormone secretagogue receptor (GHS-R). GHS-R is found in various tissues, but its function is unknown. Here we show that GHS-R is found in hepatoma cells. Exposure of these cells to ghrelin caused up-regulation of several insulin-induced ... More
Transcriptional repression of the anti-apoptotic survivin gene by wild type p53.
Authors Hoffman William H; Biade Siham; Zilfou Jack T; Chen Jiandong; Murphy Maureen;
JournalJ Biol Chem
PubMed ID11714700
'Survivin is a member of the inhibitor of apoptosis family. This apoptosis inhibitor also has an evolutionarily conserved role as a mitotic spindle checkpoint protein. Previous studies on p53-repressed genes have implicated several genes involved in the G(2)/M transition of the cell cycle as targets of negative regulation by p53. ... More
Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.
AuthorsPenna G, Fibbi B, Amuchastegui S, Cossetti C, Aquilano F, Laverny G, Gacci M, Crescioli C, Maggi M, Adorini L,
JournalJ Immunol
PubMed ID19299703
'Benign prostatic hyperplasia (BPH), a highly prevalent prostatic condition, could involve an inflammatory component in disease pathogenesis. In this study, we show that human stromal prostate cells obtained from BPH tissue can actively contribute to the inflammatory process by secreting proinflammatory cytokines as well as chemokines able to recruit lymphomonuclear ... More
Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination.
Authors Von Budingen Hans-Christian; Hauser Stephen L; Fuhrmann Antje; Nabavi Cameron B; Lee Joy I; Genain Claude P;
JournalProc Natl Acad Sci U S A
PubMed ID12060766
'Myelin/oligodendrocyte glycoprotein (MOG) is a target antigen for myelin-destructive Abs in autoimmune central nervous system demyelinating disorders. Little is known about the molecular and structural basis of these pathogenic Ab responses. Here, we have characterized anti-MOG Ab specificities in the marmoset model of experimental allergic encephalomyelitis, by means of a ... More
Marked perinatal lethality and cellular signaling deficits in mice null for the two sphingosine 1-phosphate (S1P) receptors, S1P(2)/LP(B2)/EDG-5 and S1P(3)/LP(B3)/EDG-3.
Authors Ishii Isao; Ye Xiaoqin; Friedman Beth; Kawamura Shuji; Contos James J A; Kingsbury Marcy A; Yang Amy H; Zhang Guangfa; Brown Joan Heller; Chun Jerold;
JournalJ Biol Chem
PubMed ID12006579
'Five cognate G protein-coupled receptors (S1P(1-5)) have been shown to mediate various cellular effects of sphingosine 1-phosphate (S1P). Here we report the generation of mice null for S1P(2) and for both S1P(2) and S1P(3). S1P(2)-null mice were viable and fertile and developed normally. The litter sizes from S1P(2)S1P(3) double-null crosses ... More
Cloning and characterization of a family of proteins associated with Mpl.
'Thrombopoietin (TPO) controls the formation of megakaryocytes and platelets from hematopoietic stem cells via activation of the c-Mpl receptor and multiple downstream signal transduction pathways. We used two-hybrid screening to identify new proteins that interacted with the cytoplasmic domain of Mpl, and we found a new family of proteins designated ... More
Chronic ethanol increases lipopolysaccharide-stimulated Egr-1 expression in RAW 264.7 macrophages: contribution to enhanced tumor necrosis factor alpha production.
Authors Shi Liang; Kishore Raj; McMullen Megan R; Nagy Laura E;
JournalJ Biol Chem
PubMed ID11856733
'Increased production of tumor necrosis factor alpha (TNFalpha) is associated with the development of alcoholic liver disease. Culture of RAW264.7 macrophages with 25 mm ethanol for 48 h increased lipopolysaccharide (LPS)-stimulated accumulation of tumor necrosis factor alpha (TNFalpha) peptide and mRNA by 2-fold. We investigated whether chronic ethanol-induced increases in ... More
Retroviral delivery of connexin genes to human breast tumor cells inhibits in vivo tumor growth by a mechanism that is independent of significant gap junctional intercellular communication.
Authors Qin Hong; Shao Qing; Curtis Heather; Galipeau Jacques; Belliveau Daniel J; Wang Taiqi; Alaoui-Jamali Moulay A; Laird Dale W;
JournalJ Biol Chem
PubMed ID12042301
'The mechanism by which gap junction proteins, connexins, act as potent tumor suppressors remains poorly understood. In this study human breast tumor cells were found to exhibit diverse gap junction phenotypes including (a) undetectable Cx43 and no intercellular communication (HBL100); (b) low levels of Cx43 and sparse intercellular communication (MDA-MB-231); ... More
Roles of phosphatidylinositol 3-kinase in interferon-gamma-dependent phosphorylation of STAT1 on serine 727 and activation of gene expression.
Authors Nguyen H; Ramana C V; Bayes J; Stark G R;
JournalJ Biol Chem
PubMed ID11438544
'STAT1 must be phosphorylated on serine 727 to be fully active in transcription. We show that phosphatidylinositol 3-kinase (PI3K) and its effector kinase Akt play an important role in the serine phosphorylation of STAT1 and in the activation of gene expression in response to interferon-gamma (IFN gamma). IFN gamma activates ... More
Insulin Activates CCAAT/Enhancer Binding Proteins and Proinflammatory Gene Expression through the Phosphatidylinositol 3-Kinase Pathway in Vascular Smooth Muscle Cells.
'Phosphatidylinositol 3-kinase (PI3K) is a key molecule mediating signals of insulin in vascular smooth muscle cells (VSMCs). To examine the effect of chronic activation of PI3K on the gene expression of VSMCs, membrane-targeted p110CAAX, a catalytic subunit of PI3K, was overexpressed in rat VSMCs by adenovirus-mediated gene transfer. Similar to ... More
B cells develop in the zebrafish pancreas.
Authors Danilova Nadia; Steiner Lisa A;
JournalProc Natl Acad Sci U S A
PubMed ID12370418
'The zebrafish, with its transparent free-living embryo, is a useful organism for investigating early stages in lymphopoiesis. Previously, we showed that T cells differentiate in the thymus by day 4, but no sites for B cell differentiation were seen until 3 weeks. We report here that on day 4, we ... More
Differentiation of embryonic stem cells is induced by GATA factors.
'Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis. The essential roles of Gata-6 and Gata-4 on differentiation of visceral endoderm have been identified by analyses of knockout ... More
Human ABCA1 BAC transgenic mice show increased high density lipoprotein cholesterol and ApoAI-dependent efflux stimulated by an internal promoter containing liver X receptor response elements in intron 1.
Authors Singaraja R R; Bocher V; James E R; Clee S M; Zhang L H; Leavitt B R; Tan B; Brooks-Wilson A; Kwok A; Bissada N; Yang Y Z; Liu G; Tafuri S R; Fievet C; Wellington C L; Staels B; Hayden M R;
JournalJ Biol Chem
PubMed ID11423537
'By using BAC transgenic mice, we have shown that increased human ABCA1 protein expression results in a significant increase in cholesterol efflux in different tissues and marked elevation in high density lipoprotein (HDL)-cholesterol levels associated with increases in apoAI and apoAII. Three novel ABCA1 transcripts containing three different transcription initiation ... More
Interaction of intracellular beta amyloid peptide with chaperone proteins.
Authors Fonte Virginia; Kapulkin Vadim; Taft Andrew; Fluet Amy; Friedman David; Link Christopher D;
JournalProc Natl Acad Sci U S A
PubMed ID12089340
'Expression of the human beta amyloid peptide (A beta) in transgenic Caenorhabditis elegans animals can lead to the formation of intracellular immunoreactive deposits as well as the formation of intracellular amyloid. We have used this model to identify proteins that interact with intracellular A beta in vivo. Mass spectrometry analysis ... More
Estradiol differentially regulates lipocalin-type prostaglandin D synthase transcript levels in the rodent brain: Evidence from high-density oligonucleotide arrays and in situ hybridization.
AuthorsMong JA, Devidze N, Frail DE, O'Connor LT, Samuel M, Choleris E, Ogawa S, Pfaff DW,
JournalProc Natl Acad Sci U S A
PubMed ID12518068
'Microarrays comprise an efficient approach to discovering large numbers of differentially expressed mRNA transcripts in the CNS resulting from changes in hormonal milieu. We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E(2)) on the transcriptomes from the medial basal hypothalamus and other brain regions ... More
Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.
Authors Vazquez G; Lievremont J P; St J Bird G; Putney J W Jr;
JournalProc Natl Acad Sci U S A
PubMed ID11553786
'Mammalian Trp proteins are candidates for plasma membrane calcium channels regulated by receptor activation or by intracellular calcium store depletion [capacitative calcium entry (CCE)]. One extensively investigated member of the Trp family, the human Trp3 (hTrp3), behaves as a receptor-activated, calcium-permeable, nonselective cation channel when expressed in cell lines and ... More
Recycling of RNA binding iron regulatory protein 1 into an aconitase after nitric oxide removal depends on mitochondrial ATP.
'Iron regulatory proteins (IRPs) control iron metabolism by specifically interacting with iron-responsive elements (IREs) on mRNAs. Nitric oxide (NO) converts IRP-1 from a [4Fe-4S] aconitase to a trans-regulatory protein through Fe-S cluster disassembly. Here, we have focused on the fate of IRE binding IRP1 from murine macrophages when NO flux ... More
Src Family Kinases Phosphorylate Protein Kinase C delta on Tyrosine Residues and Modify the Neoplastic Phenotype of Skin Keratinocytes.
Authors Joseloff Elizabeth; Cataisson Christophe; Aamodt Heather; Ocheni Henrietta; Blumberg Peter; Kraker Alan J; Yuspa Stuart H;
JournalJ Biol Chem
PubMed ID11812791
'Protein kinase C delta (PKC delta) is tyrosine-phosphorylated and catalytically inactive in mouse keratinocytes transformed by a ras oncogene. In several other model systems, Src kinases are upstream regulators of PKC delta. To examine this relationship in epidermal carcinogenesis, v-ras transformed mouse keratinocytes were treated with a selective Src kinase ... More
Acetylation of beta-catenin by CREB-binding protein (CBP).
Authors Wolf Daniel; Rodova Marianna; Miska Eric A; Calvet James P; Kouzarides Tony;
JournalJ Biol Chem
PubMed ID11973335
'Acetylation controls the activity of numerous proteins involved in regulating gene transcription as well as many other cellular processes. In this report we show that the CREB-binding protein (CBP) acetyltransferase acetylates beta-catenin protein in vivo. beta-Catenin is a central component of the Wnt signaling pathway, which is of key importance ... More
Defective cytochrome c-dependent caspase activation in ovarian cancer cell lines due to diminished or absent apoptotic protease activating factor-1 activity.
Authors Wolf B B; Schuler M; Li W; Eggers-Sedlet B; Lee W; Tailor P; Fitzgerald P; Mills G B; Green D R;
JournalJ Biol Chem
PubMed ID11429402
'Apoptosis via the mitochondrial pathway requires release of cytochrome c into the cytosol to initiate formation of an oligomeric apoptotic protease-activating factor-1 (APAF-1) apoptosome. The apoptosome recruits and activates caspase-9, which in turn activates caspase-3 and -7, which then kill the cell by proteolysis. Because inactivation of this pathway may ... More
Very Low Density Lipoprotein (VLDL) Receptor-deficient Mice Have Reduced Lipoprotein Lipase Activity. POSSIBLE CAUSES OF HYPERTRIGLYCERIDEMIA AND REDUCED BODY MASS WITH VLDL RECEPTOR DEFICIENCY.
Authors Yagyu Hiroaki; Lutz E Peer; Kako Yuko; Marks Steven; Hu Yunying; Choi Sungshin Y; Bensadoun Andre; Goldberg Ira J;
JournalJ Biol Chem
PubMed ID11790777
'Although very low density lipoprotein (VLDL) receptor (VLDLr) knockout mice have been reported to have no lipoprotein abnormalities, they develop less adipose tissue than control mice when fed a high calorie diet. Mice that are deficient in adipose tissue expression of lipoprotein lipase (LpL) also have less fat, but only ... More
A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.
'CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase alpha loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third ... More
Primates highly responsive to dietary cholesterol up-regulate hepatic ACAT2, and less responsive primates do not.
Authors Rudel Lawrence L; Davis Matthew; Sawyer Janet; Shah Ramesh; Wallace Jeanne;
JournalJ Biol Chem
PubMed ID12080065
'The role of liver acyl-CoA:cholesterol acyltransferase 2 (ACAT2), earlier shown to be the principal ACAT enzyme within primate hepatocytes, as a regulator of the hypercholesterolemia induced by dietary cholesterol was studied. At the end of low and high cholesterol diet periods, liver biopsies were taken from cynomolgus monkeys, a species ... More
Influence of gene action across different time scales on behavior.
Authors Ben-Shahar Y; Robichon A; Sokolowski M B; Robinson G E;
JournalScience
PubMed ID11976416
'Genes can affect natural behavioral variation in different ways. Allelic variation causes alternative behavioral phenotypes, whereas changes in gene expression can influence the initiation of behavior at different ages. We show that the age-related transition by honey bees from hive work to foraging is associated with an increase in the ... More
AuthorsChevallier J, Chamoun Z, Jiang G, Prestwich G, Sakai N, Matile S, Parton RG, Gruenberg J,
JournalJ Biol Chem
PubMed ID18644787
'Most cell types acquire cholesterol by endocytosis of circulating low density lipoprotein, but little is known about the mechanisms of intra-endosomal cholesterol transport and about the primary cause of its aberrant accumulation in the cholesterol storage disorder Niemann-Pick type C (NPC). Here we report that lysobisphosphatidic acid (LBPA), an unconventional ... More
Transcriptional regulation of the transforming growth factor beta type II receptor gene by histone acetyltransferase and deacetylase is mediated by NF-Y in human breast cancer cells.
Authors Park Seok Hee; Lee Sae Ra; Kim Byung Chul; Cho Eun Ah; Patel Sejal P; Kang Hee-Bum; Sausville Edward A; Nakanishi Osamu; Trepel Jane B; Lee Byoung Ick; Kim Seong-Jin;
JournalJ Biol Chem
PubMed ID11744689
'Transcriptional repression of the transforming growth factor-beta (TGF-beta) type II receptor (TbetaRII) gene is one of several mechanisms leading to TGF-beta resistance. Previously, we have shown that MS-275, a synthetic inhibitor of histone deacetylase (HDAC), specifically induces the expression of the TbetaRII gene and restores the TGF-beta signaling in human ... More
Coordinated transcription of key pathways in the mouse by the circadian clock.
Authors Panda Satchidananda; Antoch Marina P; Miller Brooke H; Su Andrew I; Schook Andrew B; Straume Marty; Schultz Peter G; Kay Steve A; Takahashi Joseph S; Hogenesch John B;
JournalCell
PubMed ID12015981
'In mammals, circadian control of physiology and behavior is driven by a master pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. We have used gene expression profiling to identify cycling transcripts in the SCN and in the liver. Our analysis revealed approximately 650 cycling transcripts and showed that ... More
Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus in Drosophila.
Authors Dorn R; Reuter G; Loewendorf A;
JournalProc Natl Acad Sci U S A
PubMed ID11493677
'The Drosophila BTB domain containing gene mod(mdg4) produces a large number of protein isoforms combining a common N-terminal region of 402 aa with different C termini. We have deduced the genomic structure of this complex locus and found that at least seven of the mod(mdg4) isoforms are encoded on both ... More
Functional diversity of Xenopus lymphoid enhancer factor/T-cell factor transcription factors relies on combinations of activating and repressing elements.
'Lymphoid enhancer factor/T-cell factor (LEF/TCF) high mobility group box transcription factors are the nuclear transducers of the Wnt/beta-catenin signaling cascade. In Xenopus, three members of the LEF/TCF family, XLEF-1, XTCF-3, and XTCF-4, with distinct but partially overlapping expression patterns have been identified. The individual Xenopus LEF/TCF family members differ extremely ... More
Fibroblast growth factor 18 influences proximal programming during lung morphogenesis.
Authors Whitsett Jeffrey A; Clark Jean C; Picard Lara; Tichelaar Jay W; Wert Susan E; Itoh Nobuyuki; Perl Anne-Karina T; Stahlman Mildred T;
JournalJ Biol Chem
PubMed ID11927601
'The structure and functions of the airways of the lung change dramatically along their lengths. Large-diameter conducting airways are supported by cartilaginous rings and smooth muscle tissue and are lined by ciliated and secretory epithelial cells that are involved in mucociliary clearance. Smaller peripheral airways formed during branching morphogenesis are ... More
E2Ls, E2F-like repressors of Arabidopsis that bind to E2F sites in a monomeric form.
Authors Kosugi Shunichi; Ohashi Yuko;
JournalJ Biol Chem
PubMed ID11867638
'E2F transcription factors are major regulators of cell proliferation, and each factor contributes differently to cell cycle control. Arabidopsis contains six E2F homologs, of which three are proteins that exhibit an overall similarity to animal E2Fs and interact with DPa and DPb to stimulate DNA binding. Here we describe the ... More
Gene expression profiling of isogenic cells with different TP53 gene dosage reveals numerous genes that are affected by TP53 dosage and identifies CSPG2 as a direct target of p53.
Authors Yoon Heejei; Liyanarachchi Sandya; Wright Fred A; Davuluri Ramana; Lockman Janet C; De La Chapelle Albert; Pellegata Natalia S;
JournalProc Natl Acad Sci U S A
PubMed ID12438652
'TP53 does not fully comply with the Knudson model [Knudson, A. G., Jr. (1971) Proc. Natl. Acad. Sci. USA 68, 820-823] in that a reduction of constitutional expression of p53 may be sufficient for tumor predisposition. This finding suggests a gene-dosage effect for p53 function. To determine whether TP53 gene ... More
3-Hydroxykynurenine Transaminase Identity with Alanine Glyoxylate Transaminase. A PROBABLE DETOXIFICATION PROTEIN IN AEDES AEGYPTI.
Authors Han Qian; Fang Jianmin; Li Jianyong;
JournalJ Biol Chem
PubMed ID11880382
'This study describes the functional characterization of a specific mosquito transaminase responsible for catalyzing the transamination of 3-hydroxykynurenine (3-HK) to xanthurenic acid (XA). The enzyme was purified from Aedes aegypti larvae by ammonium sulfate fractionation, heat treatment, and various chromatographic techniques, plus non-denaturing electrophoresis. The purified transaminase has a relative ... More
Fibrin inhibits peripheral nerve remyelination by regulating schwann cell differentiation.
'Remyelination is a critical step for functional nerve regeneration. Here we show that fibrin deposition in the peripheral nervous system after injury is a key regulator of remyelination. After sciatic nerve crush, fibrin is deposited and its clearance correlates with remyelination. Fibrin induces phosphorylation of ERK1/2 and production of p75 ... More
Identifying novel transcripts and novel genes in the human genome by using novel SAGE tags.
Authors Chen Jianjun; Sun Miao; Lee Sanggyu; Zhou Guolin; Rowley Janet D; Wang San Ming;
JournalProc Natl Acad Sci U S A
PubMed ID12213963
'The number of genes in the human genome is still a controversial issue. Whereas most of the genes in the human genome are said to have been physically or computationally identified, many short cDNA sequences identified as tags by use of serial analysis of gene expression (SAGE) do not match ... More
Gene Transfection of H25A Mutant Heme Oxygenase-1 Protects Cells against Hydroperoxide-induced Cytotoxicity.
'Heme oxygenase (HO)-1 is a stress-inducible enzyme protecting cells against oxidative stress, and mechanisms have been considered to depend exclusively on its enzyme activity. This study aimed to examine if the protein lacking catalytic activities could also display such resistance against oxidative stress. Stable transfectants of rat wild type HO-1 ... More
Manganese(II) induces apoptotic cell death in NIH3T3 cells via a caspase-12-dependent pathway.
Authors Oubrahim Hammou; Chock P Boon; Stadtman Earl R;
JournalJ Biol Chem
PubMed ID11964391
'Under physiological conditions, manganese(II) exhibits catalase-like activity. However, at elevated concentrations, it induces apoptosis via a non-mitochondria-mediated mechanism (Oubrahim, H., Stadtman, E. R., and Chock, P. B. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 9505-9510). In this study, we show that the Mn(II)-induced apoptosis, as monitored by caspase-3-like ... More
Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy.
Authors Gomes M D; Lecker S H; Jagoe R T; Navon A; Goldberg A L;
JournalProc Natl Acad Sci U S A
PubMed ID11717410
'Muscle wasting is a debilitating consequence of fasting, inactivity, cancer, and other systemic diseases that results primarily from accelerated protein degradation by the ubiquitin-proteasome pathway. To identify key factors in this process, we have used cDNA microarrays to compare normal and atrophying muscles and found a unique gene fragment that ... More
Collaboration of JNKs and ERKs in nerve growth factor regulation of the neurofilament light chain promoter in PC12 cells.
'Nerve growth factor (NGF) induces transcription-dependent neural differentiation of PC12 cells, and the ERK family of MAPKs has been implicated as the dominant signal pathway that mediates this response. We employed a neurofilament light chain (NFLC) promoter-luciferase (NFLC-Luc) reporter to define the role of the ERKs as well as additional ... More
Regulated expression of the apolipoprotein E/C-I/C-IV/C-II gene cluster in murine and human macrophages. A critical role for nuclear liver X receptors alpha and beta.
Authors Mak Puiying A; Laffitte Bryan A; Desrumaux Catherine; Joseph Sean B; Curtiss Linda K; Mangelsdorf David J; Tontonoz Peter; Edwards Peter A;
JournalJ Biol Chem
PubMed ID12032151
Lipid-loaded macrophage
Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.
Authors Fehlner-Gardiner Christine; Roshick Christine; Carlson John H; Hughes Scott; Belland Robert J; Caldwell Harlan D; McClarty Grant;
JournalJ Biol Chem
PubMed ID12011099
Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the
Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences.
Authors Ma M; Benimetskaya L; Lebedeva I; Dignam J; Takle G; Stein C A;
JournalNat Biotechnol
PubMed ID10625392
Most antisense oligonucleotide experiments are performed with molecules containing RNase H-competent backbones. However, RNase H may cleave nontargeted mRNAs bound to only partially complementary oligonucleotides. Decreasing such ... More
Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex.
Authors Newby Linda J; Streets Andrew J; Zhao Yan; Harris Peter C; Ward Christopher J; Ong Albert C M;
JournalJ Biol Chem
PubMed ID11901144
The functions of the two proteins defective in autosomal dominant polycystic kidney disease, polycystin-1 and polycystin-2, have not been fully clarified, but it has been hypothesized that they may heterodimerize to form a ... More
TRPC4 Can Be Activated by G-protein-coupled Receptors and Provides Sufficient Ca2+ to Trigger Exocytosis in Neuroendocrine Cells.
Authors Obukhov Alexander G; Nowycky Martha C;
JournalJ Biol Chem
PubMed ID11856742
Transient receptor potential (TRP) channels form a large family of plasma membrane cation channels. Mammalian members of the
Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia.
Authors McClain Donald A; Lubas William A; Cooksey Robert C; Hazel Mark; Parker Glendon J; Love Dona C; Hanover John A;
JournalProc Natl Acad Sci U S A
PubMed ID12136128
Insulin resistance and beta cell toxicity are key features of type 2 diabetes. One leading hypothesis suggests that these abnormalities result from excessive flux of nutrients through the UDP-hexosamine biosynthetic pathway leading to ... More
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called ... More
CADD, a Chlamydia Protein That Interacts with Death Receptors.
Authors Stenner-Liewen Frank; Liewen Heike; Zapata Juan M; Pawlowski Krzysztof; Godzik Adam; Reed John C;
JournalJ Biol Chem
PubMed ID11805081
We report here the identification of a bacterial protein capable of interacting with mammalian death receptors in vitro and in vivo. The protein is encoded in the genome of Chlamydia trachomatis and has homologues in other Chlamydia species. This protein, which we refer to as ... More
A Requirement for the CD44 Cytoplasmic Domain for Hyaluronan Binding, Pericellular Matrix Assembly, and Receptor-mediated Endocytosis in COS-7 Cells.
Authors Jiang Hong; Peterson Richard S; Wang Weihua; Bartnik Eckart; Knudson Cheryl B; Knudson Warren;
JournalJ Biol Chem
PubMed ID11792695
CD44-negative COS-7 cells were transfected with expression constructs for CD44H (the predominant CD44 isoform), CD44E (epithelial isoform), or truncation mutant derivatives lacking the carboxyl-terminal 67 amino acids of the cytoplasmic domain, CD44HDelta67 and CD44EDelta67. The truncation mutant CD44HDelta67 is identical to a naturally occurring alternatively spliced ... More
Requirement of the Activation-Induced Deaminase (AID) Gene for Immunoglobulin Gene Conversion.
Three phenotypically distinct processes-somatic hypermutation, gene conversion, and switch recombination-remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken ... More
Cid13 Is a Cytoplasmic Poly(A) Polymerase that Regulates Ribonucleotide Reductase mRNA.
Authors Saitoh Shigeaki; Chabes Andrei; McDonald W Hayes; Thelander Lars; Yates John R; Russell Paul;
JournalCell
PubMed ID12062100
Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate all mRNA, ... More
Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord.
In the developing spinal cord, signals from the roof plate are required for the development of three classes of dorsal interneuron: D1, D2, and D3, listed from dorsal to ventral. Here, we demonstrate that absence of Wnt1 and Wnt3a, normally expressed in the roof plate, leads to diminished development of ... More
Visualization and identification of IL-7 producing cells in reporter mice.
AuthorsMazzucchelli RI, Warming S, Lawrence SM, Ishii M, Abshari M, Washington AV, Feigenbaum L, Warner AC, Sims DJ, Li WQ, Hixon JA, Gray DH, Rich BE, Morrow M, Anver MR, Cherry J, Naf D, Sternberg LR, McVicar DW, Farr AG, Germain RN, Rogers K, Jenkins NA, Copeland NG, Durum SK,
JournalPLoS One
PubMed ID19907640
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside ... More
A natural product that lowers cholesterol as an antagonist ligand for FXR.
Authors Urizar Nancy L; Liverman Amy B; Dodds D'Nette T; Silva Frank Valentin; Ordentlich Peter; Yan Yingzhuo; Gonzalez Frank J; Heyman Richard A; Mangelsdorf David J; Moore David D;
JournalScience
PubMed ID11988537
Extracts of the resin of the guggul tree (Commiphora mukul) lower LDL (low-density lipoprotein) cholesterol levels in humans. The plant sterol guggulsterone [4,17(20)-pregnadiene-3,16-dione] is the active agent in this extract. We show that guggulsterone is a highly efficacious antagonist of the farnesoid X receptor (FXR), a nuclear hormone receptor that ... More