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Invitrogen

CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™

6 Published Figures
9 References
View all (48) TNFR1 antibodies

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Product Details

16-1202-050

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

-
View 3 publications 3 publications

Neutralization (Neu)

Assay-Dependent
View 6 publications 6 publications

Functional Assay (Functional)

Assay-Dependent
-
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

55R-170

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Amount

50 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865844

Product Specific Information

Description: The 55R-170 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor I (TNFRI, TNFR-p55, TNFRSF1A, CD120a). TNFRI is expressed in a variety of cell types. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.

Applications Reported:The 55R-170 antibody has been reported for use in ELISA, neutralization of mouse TNFR I bioactivity and flow cytometric analysis.

Applications Tested: The Functional Grade Purified 55R-170 antibody has been tested by LAL assay to verify low endotoxin levels and in bioassay for neutralization of mouse TNFR I bioactivity.

The 55R-170 antibody at 25 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:Less than 10%, as determined by HPLC.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD120a antigen; MGC19588; n-smase activation assoc. factor; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF receptor alpha chain; TNF-alpha-R1; TNF-RI; TNFR; TNFR I; TNFR-I; tnfrsf1a; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I; unnamed protein product

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Gene Aliases: CD120a; FPF; p55; p55-R; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFalpha-R1; TNFAR; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55

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UniProt ID: (Mouse) P25118

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Entrez Gene ID: (Mouse) 21937

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Function(s)
protease binding tumor necrosis factor-activated receptor activity protein binding signaling receptor activity identical protein binding tumor necrosis factor binding macromolecular complex binding
Process(es)
aortic valve development pulmonary valve development negative regulation of extracellular matrix constituent secretion prostaglandin metabolic process apoptotic process defense response inflammatory response signal transduction cell surface receptor signaling pathway JAK-STAT cascade extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to bacterium gene expression regulation of gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression regulation of tumor necrosis factor-mediated signaling pathway cytokine-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of tumor necrosis factor production tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling positive regulation of protein import into nucleus defense response to bacterium regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter astrocyte activation negative regulation of inflammatory response positive regulation of inflammatory response regulation of developmental process negative regulation of multicellular organismal process establishment of blood-brain barrier cellular response to lipopolysaccharide cellular response to mechanical stimulus protein localization to plasma membrane apoptotic signaling pathway positive regulation of execution phase of apoptosis positive regulation of apoptotic process involved in morphogenesis regulation of establishment of endothelial barrier
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