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CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™
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-Functional-Grade-Flow-20170216130641.jpg?time=20240507 "CD120a (TNF Receptor I) Antibody in Functional Assay (Functional)")
FIGURE: 1 / 7
CD120a (TNF Receptor I) Antibody (16-1202-050) in Functional
Anti-Mouse CD120a (TNF Receptor I) Functional Grade Purified inhibits the biological effects of recombinant mouse TNF alpha in a bioassay of TNF alpha induced cytotoxicity of the L929 cell line. Antibody concentration is in µg/mL.
-Functional-Grade-Flow-20170216130641.jpg?time=20240507)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
-Functional-Grade-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD120a (TNF Receptor I) Antibody (16-1202-050) in Functional")
Product Details
16-1202-050
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
55R-170
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
50 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 55R-170 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor I (TNFRI, TNFR-p55, TNFRSF1A, CD120a). TNFRI is expressed in a variety of cell types. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.
Applications Reported:The 55R-170 antibody has been reported for use in ELISA, neutralization of mouse TNFR I bioactivity and flow cytometric analysis.
Applications Tested: The Functional Grade Purified 55R-170 antibody has been tested by LAL assay to verify low endotoxin levels and in bioassay for neutralization of mouse TNFR I bioactivity.
The 55R-170 antibody at 25 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD120a antigen; MGC19588; n-smase activation assoc. factor; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF receptor alpha chain; TNF-alpha-R1; TNF-RI; TNFR; TNFR I; TNFR-I; tnfrsf1a; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I; unnamed protein product
Gene Aliases: CD120a; FPF; p55; p55-R; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFalpha-R1; TNFAR; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55
UniProt ID: (Mouse) P25118
Entrez Gene ID: (Mouse) 21937
aortic valve development
pulmonary valve development
negative regulation of extracellular matrix constituent secretion
prostaglandin metabolic process
apoptotic process
defense response
inflammatory response
signal transduction
cell surface receptor signaling pathway
JAK-STAT cascade
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
response to bacterium
gene expression
regulation of gene expression
negative regulation of cardiac muscle hypertrophy
positive regulation of gene expression
negative regulation of gene expression
regulation of tumor necrosis factor-mediated signaling pathway
cytokine-mediated signaling pathway
negative regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
positive regulation of protein import into nucleus
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
astrocyte activation
negative regulation of inflammatory response
positive regulation of inflammatory response
regulation of developmental process
negative regulation of multicellular organismal process
establishment of blood-brain barrier
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
protein localization to plasma membrane
apoptotic signaling pathway
positive regulation of execution phase of apoptosis
positive regulation of apoptotic process involved in morphogenesis
regulation of establishment of endothelial barrier
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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