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Invitrogen
CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), Functional Grade, eBioscience™
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Product Details
16-3391-025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HMJ1-29
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
25 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This HMJ1-29 monoclonal antibody reacts with mouse Jagged-1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Jagged-1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-1 is expressed by thymic lymphoid and stromal cells, as well as macrophages and CD8+CD11c+ dendritic cells in the spleen.
The HMJ1-29 antibody is reported to crossreact to rat.
Applications Reported: This HMD1-5 antibody has been reported for use in functional assays.
Applications Tested: This HMJ1-29 antibody has been tested by flow cytometric analysis of Jagged-1-transfected CHO cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: jagged 1; jagged canonical Notch ligand 1; Serrate-1; unnamed protein product
Gene Aliases: ABE2; Gena228; Gsfabe2; Htu; Ozz; Ser-1
UniProt ID: (Mouse) Q9QXX0, (Rat) Q63722
Entrez Gene ID: (Mouse) 16449, (Rat) 29146
negative regulation of cell-matrix adhesion
blood vessel remodeling
morphogenesis of an epithelium
morphogenesis of an epithelial sheet
T cell mediated immunity
pulmonary valve morphogenesis
cardiac right ventricle morphogenesis
cell communication
Notch signaling pathway
organ morphogenesis
regulation of gene expression
positive regulation of gene expression
negative regulation of cell-cell adhesion
negative regulation of cell migration
response to muramyl dipeptide
tube morphogenesis
aorta morphogenesis
regulation of cell proliferation
auditory receptor cell differentiation
camera-type eye development
negative regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of fat cell differentiation
negative regulation of endothelial cell differentiation
positive regulation of myeloid cell differentiation
negative regulation of neuron differentiation
positive regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
cell development
inner ear development
regulation of multicellular organismal process
cardiac septum morphogenesis
ciliary body morphogenesis
pulmonary artery morphogenesis
cardiac neural crest cell development involved in outflow tract morphogenesis
endocardial cushion cell development
positive regulation of cardiac epithelial to mesenchymal transition
nephron development
glomerular visceral epithelial cell development
distal tubule development
loop of Henle development
sensory organ morphogenesis
neuronal stem cell population maintenance
negative regulation of stem cell differentiation
cell fate determination
regeneration
organ regeneration
wound healing
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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