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Invitrogen
Ly-6C Monoclonal Antibody (HK1.4), Functional Grade, eBioscience™
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FIGURE: 1 / 80
Ly-6C Antibody (16-5932-85) in Flow
Investigative ophthalmology & visual science 2023 -
Figure 5. Splenectomy results with a change in the distribution of Ly6C hi monocytes in the cornea, peripheral blood, and bone marrow after but not before corneal abrasion. ( A ) Distribution of Ly6C hi monocytes in the resting cornea of animals whose spleen was surgically removed and those that were sham-operated ( left ). Representative plots of the flow cytometry analysis of the resting cornea ( right ).... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-5932-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2c, kappa
Class
Monoclonal
Type
Antibody
Clone
HK1.4
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.
In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.
Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis and functional assays. (Fluorochrome-conjugated HK1.4 is recommended for use in flow cytometry).
Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; Lymphocyte antigen Ly-6C; unnamed protein product
Gene Aliases: Ly-6C; Ly-6C1; Ly6c
UniProt ID: (Mouse) P0CW02
Entrez Gene ID: (Mouse) 17067
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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