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Invitrogen

IFN gamma Monoclonal Antibody (H22), Functional Grade, eBioscience™

2 Published Figures
4 References
View all (237) IFN gamma antibodies

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Product Details

16-7411-85

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

-
View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent
View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H22

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Amount

500 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573117

Product Specific Information

Description: This H22 monoclonal antibody reacts with and inhibits the activity of Mouse IFN gamma. Mouse IFN gamma is a 20 kDa factor produced by activated T, B, and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma.

Applications Reported: This H22 monoclonal antibody inhibits the activity of Mouse IFN gamma and is reported to have a 14 day half-life when used in vivo.

Applications Tested: The ND50 of H22, as measured by the inhibition of protection against EMC virus in L929 cells, is less than or equal to 50 ng/mL in the presence of 1 ng/mL of Mouse IFN gamma Recombinant Protein. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cytokine; gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; interferon 2f; interferon gamma precursor; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g; unnamed protein product

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Gene Aliases: If2f; Ifg; IFN-g

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)
cytokine activity interferon-gamma receptor binding protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation neutrophil apoptotic process positive regulation of cytokine production regulation of the force of heart contraction adaptive immune response macrophage activation involved in immune response CD8-positive, alpha-beta T cell differentiation involved in immune response positive regulation of T cell cytokine production regulation of transcription, DNA-templated apoptotic process inflammatory cell apoptotic process immune response humoral immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration urea metabolic process antigen processing and presentation macrophage differentiation neutrophil chemotaxis negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response positive regulation of protein complex assembly negative regulation of myelination regulation of neurological system process positive regulation of synaptic transmission, cholinergic negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process Fc-gamma receptor signaling pathway involved in phagocytosis interleukin-2-mediated signaling pathway type III interferon-mediated signaling pathway positive regulation of T cell proliferation macrophage activation positive regulation of protein import into nucleus positive regulation of tyrosine phosphorylation of STAT protein defense response to bacterium defense response to protozoan positive regulation of apoptotic process positive regulation by host of viral process positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation negative regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of glycolytic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter creatinine metabolic process astrocyte activation negative regulation of fibroblast proliferation positive regulation of isotype switching to IgG isotypes negative regulation of smooth muscle cell proliferation regulation of defense response to virus by host positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of neurogenesis regulation of immune response regulation of insulin secretion defense response to Gram-positive bacterium T cell receptor signaling pathway sensory perception of mechanical stimulus positive regulation of membrane protein ectodomain proteolysis defense response to virus regulation of glial cell proliferation interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process cellular response to lipopolysaccharide cellular response to interleukin-18 negative regulation of glomerular mesangial cell proliferation extrinsic apoptotic signaling pathway receptor signaling pathway via STAT regulation of neuronal action potential neuroinflammatory response negative regulation of beta-amyloid clearance positive regulation of glutamate receptor signaling pathway positive regulation of cellular respiration positive regulation of beta-amyloid formation response to sodium arsenite positive regulation of iron ion import across plasma membrane positive regulation of STAT cascade positive regulation of tumor necrosis factor (ligand) superfamily member 11 production regulation of hepatocyte proliferation
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