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Invitrogen
IL-15/IL-15R Complex Monoclonal Antibody (GRW15PLZ), Functional Grade, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-8156-025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
GRW15PLZ
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
25 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody GRW15PLZ recognizes the mouse IL-15/IL-15 receptor complex. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that is has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.
Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted when in complex with its unique receptor, IL-15R alpha. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.
Applications Reported: The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.
Applications Tested: The ND50 of GRW15PLZ, as determined by the inhibition of CTLL-2 cell proliferation, is 15-60 ng/mL in the presence of 6 ng/mL recombinant mouse IL-15/IL-15R complex, or 0.4-0.8 µg/mL in the presence of 120 ng/mL recombinant mouse IL-15. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine isoform; IL-15 receptor subunit alpha; IL-15R-alpha; IL15ra; ILN; Interleukin; unnamed protein product
Gene Aliases: IL-15; IL-15RA
UniProt ID: (Mouse) P48346, (Mouse) Q60819
Entrez Gene ID: (Mouse) 16168, (Mouse) 16169
positive regulation of cytokine production
NK T cell proliferation
immune response
tyrosine phosphorylation of STAT protein
positive regulation of cell proliferation
hyaluronan metabolic process
macrophage differentiation
positive regulation of protein modification process
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of natural killer cell differentiation
interleukin-15-mediated signaling pathway
positive regulation of T cell proliferation
neutrophil activation
extrathymic T cell selection
regulation of T cell differentiation
cell maturation
lymph node development
negative regulation of smooth muscle cell proliferation
regulation of defense response to virus by host
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of phagocytosis
positive regulation of immune response
negative regulation of cold-induced thermogenesis
positive regulation of protein O-linked glycosylation
JAK-STAT cascade
negative regulation of neuron projection development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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