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Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), APC, eBioscience™

Advanced Verification
46 Published Figures
44 References
View all (97) FLK1 (VEGF Receptor 2) antibodies

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Additional Information:

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Product Details

17-5821-80

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test
View 42 publications 42 publications

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Avas12a1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657865

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: This Avas12a1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD309 antigen; fetal liver kinase 1; Flk-1; kinase NYK; platelet-derived growth factor receptor-related tyrosine kinase; protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; unnamed protein product; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor; VEGF receptor-2

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Gene Aliases: 6130401C07; Flk-1; Flk1; Krd-1; Ly73; orv; sVEGFR-2; VEGFR-2; VEGFR2

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UniProt ID: (Mouse) P35918

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Entrez Gene ID: (Mouse) 16542

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Function(s)
protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding coreceptor activity growth factor binding vascular endothelial growth factor binding identical protein binding cadherin binding
Process(es)
angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia positive regulation of protein phosphorylation regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation lymph vessel development positive regulation of mesenchymal cell proliferation negative regulation of systemic arterial blood pressure endocardium development endothelium development endochondral bone growth transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of cell proliferation regulation of cell shape gonad development male gonad development response to xenobiotic stimulus positive regulation of signal transduction positive regulation of endothelial cell migration negative regulation of gene expression positive regulation of macroautophagy cell migration peptidyl-tyrosine phosphorylation hemopoiesis lung development positive regulation of cell migration positive regulation of BMP signaling pathway positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of TOR signaling embryonic hemopoiesis cellular response to vascular endothelial growth factor stimulus vascular endothelial growth factor receptor-2 signaling pathway vascular endothelial growth factor signaling pathway vasodilation surfactant homeostasis regulation of MAPK cascade positive regulation of MAPK cascade negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration cell fate commitment endothelial cell differentiation positive regulation of angiogenesis protein autophosphorylation vascular endothelial growth factor receptor signaling pathway positive regulation of long-term neuronal synaptic plasticity lung alveolus development cell maturation blood vessel morphogenesis post-embryonic camera-type eye morphogenesis branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of epithelial cell proliferation positive regulation of calcium-mediated signaling positive regulation of positive chemotaxis positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling positive regulation of mitochondrial depolarization calcium ion homeostasis epithelial tube morphogenesis blood vessel endothelial cell differentiation vascular wound healing endothelial tube morphogenesis positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway positive regulation of cell migration involved in sprouting angiogenesis positive regulation of mitochondrial fission positive regulation of nitric oxide-cGMP mediated signal transduction regulation of hematopoietic progenitor cell differentiation regulation of bone development cellular response to hydrogen sulfide negative regulation of endothelial cell apoptotic process positive regulation of endothelial cell chemotaxis positive regulation of vasculogenesis
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