Lipofectin™ Transfection Reagent
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Citas y referencias (276)
Invitrogen™

Lipofectin™ Transfection Reagent

El reactivo de transfección Lipofectin® es el reactivo de elección para la transfección de células endoteliales. El reactivo de transfecciónMás información
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Número de catálogoCantidad
182920111 mL
182920374 mL
Número de catálogo 18292011
Precio (MXN)
-
Cantidad:
1 mL
El reactivo de transfección Lipofectin® es el reactivo de elección para la transfección de células endoteliales. El reactivo de transfección Lipofectin® también es adecuado para transfectar ADN, ARN y oligonucleótidos en células de mamíferos, y ADN en protoplastos de plantas. También se ha demostrado que el reactivo Lipofectin® funciona bien, en combinación con el reactivo PLUS®, para la transfección de células HeLa. El reactivo de transfección Lipofectin® es una formulación liposoma 1:1 (p/p) del lípido catiónico N-[1-(2,3-dioleyloxy)propil]-n,n-trimetilamonio cloruro (DOTMA) y dioleoyl fofotidiletanolamina (DOPE) en agua filtrada por membrana.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (aplicación)Transfección
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
IncluyeUn vial (1 ml) de reactivo de transfección Lipofectin
Línea de productosLipofectin
Tipo de productoReactivos para transfección
Cantidad1 mL
Compatible con sueroNo
Condiciones de envíoHielo húmedo
Tipo de célulaLíneas de células establecidas, células primarias, células difíciles de transfeccionar
FormatoPlaca de 6 pocillos, placa de 12 pocillos, placa de 24 pocillos, placa de 48 pocillos, placa de 96 pocillos, matraces
Tipo de muestraADN plasmídico
Transfection TechniqueTransfección basada en lípidos
Unit SizeEach
Contenido y almacenamiento
Contiene un vial (1 ml) de reactivo de transfección Lipofectin™. Almacenar a 4°C. No la congele.

Preguntas frecuentes

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectin™ Transfection Reagent, 1 mL FAQs

Citations & References (276)

Citations & References
Abstract
Characterization of human B creatine kinase gene regulation in the heart in vitro and in vivo.
Authors:Ritchie ME
Journal:The Journal of biological chemistry
PubMed ID:8810319
Isolation and characterization of a novel cDNA encoding a human UDP-glucuronosyltransferase active on C19 steroids.
Authors:Beaulieu M,Lévesque E,Hum DW,Bélanger A
Journal:The Journal of biological chemistry
PubMed ID:8798464
Transfection techniques for producing recombinant baculoviruses.
Authors:Trotter KM,Wood HA
Journal:Molecular biotechnology
PubMed ID:9067978
Temporary ex vivo inhibition of the expression of the human oncogene HER2 (NEU) by a triple helix-forming oligonucleotide.
Authors:Porumb H; Gousset H; Letellier R; Salle V; Briane D; Vassy J; Amor-Gueret M; Israël L; Taillandier E;
Journal:Cancer Res
PubMed ID:8564964
A 28-base phosphodiester triple helix-forming oligonucleotide, mostly G and A containing, targeted to a polypurine tract interrupted by a purine-pyrimidine inversion, situated upstream from the TATA box of the promoter of the human HER2 gene, was conceived by computer modeling. The ... More
Isolation and cDNA cloning of Ksp-cadherin, a novel kidney-specific member of the cadherin multigene family.
Authors:Thomson R B; Igarashi P; Biemesderfer D; Kim R; Abu-Alfa A; Soleimani M; Aronson P S;
Journal:J Biol Chem
PubMed ID:7615566
Cadherins are recognized as the principal mediators of homotypic cellular recognition and play a demonstrated role in the morphogenic direction of tissue development. We report here the identification of a structurally unique, kidney-specific member of the cadherin multigene family (Ksp-cadherin). cDNA cloning and molecular analysis of the 130-kDa protein confirmed ... More
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