A 28-base phosphodiester triple helix-forming oligonucleotide, mostly G and A containing, targeted to a polypurine tract interrupted by a purine-pyrimidine inversion, situated upstream from the TATA box of the promoter of the human HER2 gene, was conceived by computer modeling. The ... More
Isolation and cDNA cloning of Ksp-cadherin, a novel kidney-specific member of the cadherin multigene family.
AuthorsThomson R B; Igarashi P; Biemesderfer D; Kim R; Abu-Alfa A; Soleimani M; Aronson P S;
JournalJ Biol Chem
PubMed ID7615566
Cadherins are recognized as the principal mediators of homotypic cellular recognition and play a demonstrated role in the morphogenic direction of tissue development. We report here the identification of a structurally unique, kidney-specific member of the cadherin multigene family (Ksp-cadherin). cDNA cloning and molecular analysis of the 130-kDa protein confirmed ... More
Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.
AuthorsEndo T; Ohta K; Haraguchi K; Onaya T;
JournalJ Biol Chem
PubMed ID7738021
Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More
Transfection techniques for producing recombinant baculoviruses.
AuthorsTrotter KM, Wood HA
JournalMethods Mol Biol
PubMed ID7620563
WAF1, a potential mediator of p53 tumor suppression.
Authorsel-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B
JournalCell
PubMed ID8242752
The ability of p53 to activate transcription from specific sequences suggests that genes induced by p53 may mediate its biological role as a tumor suppressor. Using a subtractive hybridization approach, we identified a gene, named WAF1, whose induction was associated with wild- type but not mutant p53 gene expression in ... More
Structure and expression of the membrane proteoglycan betaglycan, a component of the TGF-beta receptor system.
We describe the primary structure of rat betaglycan, a polymorphic membrane-anchored proteoglycan with high affinity for transforming growth factor-beta (TGF-beta). As deduced from its cDNA sequence, the 853 amino acid core protein of betaglycan has an extracellular domain with clustered sites for potential attachment of glycosaminoglycan chains. These chains are ... More
Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner.
Authors Tan Clara; Mui Alice; Dedhar Shoukat;
JournalJ Biol Chem
PubMed ID11724787
'Nitric oxide (NO) and prostaglandins are produced as a result of the stimulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2, respectively, in response to cytokines or lipopolysaccharide (LPS). We demonstrate that the activity of integrin-linked kinase (ILK) is stimulated by LPS activation in J774 macrophages. Inhibition of ILK activity ... More
Processing of Pro-atrial Natriuretic Peptide by Corin in Cardiac Myocytes.
Authors Wu Faye; Yan Wei; Pan Junliang; Morser John; Wu Qingyu;
JournalJ Biol Chem
PubMed ID11884416
'Corin is a type II transmembrane serine protease abundantly expressed in the heart. In a previous study using transfected 293 cells, we showed that corin converted pro-atrial natriuretic peptide (pro-ANP) to atrial natriuretic peptide (ANP), suggesting that corin is likely the pro-ANP convertase. Because other serine proteases such as thrombin ... More
Efficient incorporation of transfected blastodermal cells into chimeric chicken embryos.
Authors Fraser R A; Carsience R S; Clark M E; Etches R J; Gibbins A M;
JournalInt J Dev Biol
PubMed ID8292532
'The formation of transgenic chimeric chickens for use in developmental studies and as intermediates in the production of transgenic chickens requires the incorporation of stably transfected blastodermal cells into a chimera. To obtain blastodermal cells, area pellucidae of stage X (Eyal-Giladi and Kochav, Dev. Biol. 49:321-337, 1976:E.-G.&K.) embryos were collected ... More
Hypoxia-induced transcriptional activation and increased mRNA stability of vascular endothelial growth factor in C6 glioma cells.
Authors Ikeda E; Achen M G; Breier G; Risau W;
JournalJ Biol Chem
PubMed ID7544346
'Vascular endothelial growth factor (VEGF) is an endothelial specific angiogenic mitogen secreted from various cell types including tumor cells. Increasing evidence suggests that VEGF is a major regulator of physiological and pathological angiogenesis, and the VEGF/VEGF receptor system has been shown to be necessary for glioma angiogenesis. Hypoxia seems to ... More
Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Authors Iwabuchi K; Li B; Massa H F; Trask B J; Date T; Fields S;
JournalJ Biol Chem
PubMed ID9748285
'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated ... More
Isolation and characterization of the human cytochrome P450 CYP1B1 gene.
Authors Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;
JournalJ Biol Chem
PubMed ID8910454
'Previously, we identified a novel human cytochrome P450 cDNA that is inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and represents the first member of a new subfamily designated cytochrome P4501B1 (CYP1B1; Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W., ... More
Genomic structures of the human and murine corin genes and functional GATA elements in their promoters.
'Corin is a multiple-domain type II transmembrane serine protease highly expressed in the heart. It converts pro-atrial natriuretic peptide to atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. Here we describe the genomic structures of the human and murine corin genes and functional analysis of their ... More
Regulation in vivo of the growth of Leydig cell tumors by antisense ribonucleic acid for parathyroid hormone-related peptide.
Authors Rabbani S A; Gladu J; Liu B; Goltzman D;
JournalEndocrinology
PubMed ID7588290
'PTH-related peptide (PTHrP) has been shown to be the major mediator of hypercalcemia of malignancy, but may also exert effects on cell growth and differentiation. The Leydig cell tumor H-500, when implanted in Fischer rats, produces abundant PTHrP and eventually causes the death of the host animal. In the present ... More
Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.
Authors Schmedtje J F Jr; Ji Y S; Liu W L; DuBois R N; Runge M S;
JournalJ Biol Chem
PubMed ID8995303
'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction ... More
Identification of an insulin-responsive element in the rat insulin-like growth factor-binding protein-3 gene.
Authors Villafuerte B C; Zhao W; Herington A C; Saffery R; Phillips L S;
JournalJ Biol Chem
PubMed ID9030565
'The hepatic expression and serum levels of insulin-like growth factor-binding protein-3 (IGFBP-3) are decreased in insulin-dependent and insulin-resistant diabetes. Insulin increases hepatic IGFBP-3 expression by enhancing gene transcription. This report identifies sequences within the IGFBP-3 promoter that are necessary and sufficient for the response to insulin in hepatic nonparenchymal cells. ... More
Differential effects of sterol regulatory binding proteins 1 and 2 on sterol 12 alpha-hydroxylase. SREBP-2 suppresses the sterol 12 alpha-hydroxylase promoter.
Authors del Castillo-Olivares Antonio; Gil Gregorio;
JournalJ Biol Chem
PubMed ID11741989
'The most important pathway for the catabolism and excretion of cholesterol in mammals is the formation of bile acids. Improper regulation of this pathway has implications for atherosclerosis, cholesterol gallstone formation, and some lipid storage diseases. Sterol 12 alpha-hydroxylase (12 alpha-hydroxylase) is required for cholic acid biosynthesis. The alpha(1)-fetoprotein transcription ... More
Direct observation of endocytosis of gastrin releasing peptide and its receptor.
Authors Grady E F; Slice L W; Brant W O; Walsh J H; Payan D G; Bunnett N W;
JournalJ Biol Chem
PubMed ID7876231
'Endocytosis of the gastrin releasing peptide receptor (GRP-R) may regulate cellular responses to GRP. We observed endocytosis in transfected epithelial cells by confocal microscopy using cyanine 3-GRP (cyanine 3.18-labeled gastrin releasing peptide) and GRP-R antibodies. At 4 degrees C, cy3-GRP and GRP-R were confined to the plasma membrane. After 5 ... More
Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.
Authors Yoshimura K; Rosenfeld M A; Nakamura H; Scherer E M; Pavirani A; Lecocq J P; Crystal R G;
JournalNucleic Acids Res
PubMed ID1377820
'As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed of a firefly ... More
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
AuthorsCella M, Dohring C, Samaridis J, Dessing M, Brockhaus M, Lanzavecchia A, Colonna M
JournalJ Exp Med
PubMed ID9151699
'Immunoglobulin-like transcript (ILT) 3 is a novel cell surface molecule of the immunoglobulin superfamily, which is selectively expressed by myeloid antigen presenting cells (APCs) such as monocytes, macrophages, and dendritic cells. The cytoplasmic region of ILT3 contains putative immunoreceptor tyrosine-based inhibitory motifs that suggest an inhibitory function of ILT3. Indeed, ... More
Transfection of cultured cells of the cotton boll weevil, Anthonomus grandis, with a heat-shock-promoter-chloramphenicol-acetyltransferase construct.
AuthorsStiles B, Heilmann J, Sparks RB, Santoso A, Leopold RA
JournalInsect Mol Biol
PubMed ID1343780
'Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacterial ... More
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Authors Guan G; Jiang G; Koch R L; Shechter I;
JournalJ Biol Chem
PubMed ID7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More
Peroxisome proliferator-activated receptor alpha inhibits hepatic S14 gene transcription. Evidence against the peroxisome proliferator-activated receptor alpha as the mediator of polyunsaturated fatty acid regulation of s14 gene transcription.
Authors Ren B; Thelen A; Jump D B;
JournalJ Biol Chem
PubMed ID8663275
'The peroxisome proliferator-activated receptor (PPARalpha) has been implicated in fatty acid regulation of gene transcription. Lipogenic gene transcription is inhibited by polyunsaturated fatty acids (PUFA). We have used the PUFA-sensitive rat liver S14 gene as a model to examine the role PPARalpha plays in fatty acid regulation of hepatic lipogenic ... More
Endogenous retinoic acid receptor (RAR)-retinoid X receptor (RXR) heterodimers are the major functional forms regulating retinoid-responsive elements in adult human keratinocytes. Binding of ligands to RAR only is sufficient for RAR-RXR heterodimers to confer ligand-dependent activation of hRAR beta 2/RARE (DR5).
'We have examined how retinoic acid receptors (RARs) and retinoid X receptors (RXRs) at physiological concentrations regulate distinct retinoid-responsive elements, hRAR beta 2/beta RARE (DR5) and rCRBPII/RXRE (DR1), in keratinocytes from human skin, a major retinoid target. In vitro, endogenous RAR gamma and RXRs bound to these elements as heterodimers ... More
Novel polyaminolipids enhance the cellular uptake of oligonucleotides.
'Two new polyaminolipids have been synthesized for the purpose of improving cellular uptake of oligonucleotides. The amphipathic compounds are conjugates of spermidine or spermine linked through a carbamate bond to cholesterol. The polyaminolipids are relatively nontoxic to mammalian cells. In tissue culture assays, using fluorescent-tagged or radiolabeled triple helix-forming oligonucleotides, ... More
Mechanism of oligonucleotide release from cationic liposomes.
Authors Zelphati O; Szoka F C Jr;
JournalProc Natl Acad Sci U S A
PubMed ID8876163
'We propose a mechanism for oligonucleotide (ODN) release from cationic lipid complexes in cells that accounts for various observations on cationic lipid-nucleic acid-cell interactions. Fluorescent confocal microscopy of cells treated with rhodamine-labeled cationic liposome/ fluorescein-labeled ODN (F-ODN) complexes show the F-ODN separates from the lipid after internalization and enters the ... More
beta 1-Integrin-mediated Glioma Cell Adhesion and Free Radical-induced Apoptosis Are Regulated by Binding to a C-terminal Domain of PG-M/Versican.
Authors Wu Yaojiong; Chen Liwen; Zheng Peng-Sheng; Yang Burton B;
JournalJ Biol Chem
PubMed ID11805102
'Integrins are cell-surface glycoproteins that mediate cell activities, including tissue morphogenesis, development, immune response, and cancer, through interaction with extracellular proteins. Here we report a novel means by which integrin signaling and functions are regulated. In pull-down assays and immunoprecipitation, beta(1)-integrin bound to the C-terminal domain of PG-M/versican, an extracellular ... More
The intramembrane cleavage site of the amyloid precursor protein depends on the length of its transmembrane domain.
Authors Lichtenthaler Stefan F; Beher Dirk; Grimm Heike S; Wang Rong; Shearman Mark S; Masters Colin L; Beyreuther Konrad;
JournalProc Natl Acad Sci U S A
PubMed ID11805291
'Proteolytic processing of the amyloid precursor protein by beta-secretase generates C99, which subsequently is cleaved by gamma-secretase, yielding the amyloid beta peptide (A beta). This gamma-cleavage occurs within the transmembrane domain (TMD) of C99 and is similar to the intramembrane cleavage of Notch. However, Notch and C99 differ in their ... More
A small region of the natural killer cell receptor, Siglec-7, is responsible for its preferred binding to alpha 2,8-disialyl and branched alpha 2,6-sialyl residues. A comparison with Siglec-9.
Authors Yamaji Toshiyuki; Teranishi Takane; Alphey Magnus S; Crocker Paul R; Hashimoto Yasuhiro;
JournalJ Biol Chem
PubMed ID11741958
'Siglec-7 is a sialic acid-binding lectin recently identified as an inhibitory receptor on natural killer cells. Here we characterize the sugar-binding specificity of Siglec-7 expressed on Chinese hamster ovary cells using polyvalent streptavidin-based glyco-probes. Glyco-probes carrying unique oligosaccharide structures such as GD3 (NeuAc alpha 2,8NeuAc alpha 2,3Gal beta 1,4Glc) and ... More
Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line.
Authors Kekuda R; Prasad P D; Fei Y J; Torres-Zamorano V; Sinha S; Yang-Feng T L; Leibach F H; Ganapathy V;
JournalJ Biol Chem
PubMed ID8702519
'We have isolated a cDNA from a human placental choriocarcinoma cell cDNA library which, when expressed in HeLa cells, induces a Na+-dependent amino acid transport system with preference for zwitterionic amino acids. Anionic amino acids, cationic amino acids, imino acids, and N-methylated amino acids are excluded by this system. These ... More
Cationic lipid-mediated transfection of liver cells in primary culture.
Authors Jarnagin W R; Debs R J; Wang S S; Bissell D M;
JournalNucleic Acids Res
PubMed ID1508714
'We describe transfection of DNA into parenchymal and individual non-parenchymal cell populations from adult rat liver in early primary culture, using cationic lipid as the carrier. All cell populations were transfectable, although lipid requirements varied by cell type and, for hepatocytes, with the age of the culture. For hepatocytes in ... More
Cationic lipid-mediated delivery and expression of prepro-neuropeptide Y cDNA after intraventricular administration in rat: feasibility and limitations.
AuthorsThorsell A, Blomqvist AG, Heilig M
JournalRegul Pept
PubMed ID8701037
'The utility of in vivo lipofection for delivery and expression of a neuropeptide gene in the adult rat brain was explored. Prepro-neuropeptide Y (NPY) cDNA was cloned into the episomal eucaryotic expression vector pCEP4. This construct was complexed to lipofectamine or lipofectin. Complexed DNA was injected into the lateral ventricles ... More
A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.
AuthorsRose JK, Buonocore L, Whitt MA
JournalBiotechniques
PubMed ID1867862
'One of the most efficient systems for the expression of genes in the cytoplasm of animal cells utilizes a recombinant vaccinia virus encoding the bacteriophage T7 RNA polymerase. Cells infected with this virus are transfected with plasmid DNAs containing the gene to be expressed under T7 promoter control. The major ... More
Role of beta3 integrins in melanoma cell adhesion to activated platelets under flow.
AuthorsFelding-Habermann B, Habermann R, SaldÃvar E, Ruggeri ZM
JournalJ Biol Chem
PubMed ID8621462
'Mechanisms mediating tumor cell attachment to the vessel wall under flow conditions are largely unknown. Therefore we analyzed the ability of human melanoma cells to adhere to an immobilized matrix during blood flow and determined the role of platelets in this process. In a parallel plate flow chamber, M21 melanoma ... More
Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways.
'The androgen receptor (AR) is a ligand-activated transcription factor that mediates the biological responses of androgens. However, non-androgenic pathways have also been shown to activate the AR. The mechanism of cross-talk between the interleukin-6 (IL-6) and AR signal transduction pathways was investigated in LNCaP human prostate cancer cells. IL-6 induced ... More
Characterization of pseudorabies virus neutralization antigen glycoprotein gIII produced in insect cells by a baculovirus expression vector.
Authors Inumaru S; Yamada S;
JournalVirus Res
PubMed ID1661981
'The gene encoding the complete glycoprotein of pseudorabies virus (PRV, Yamagata-S81 strain glycoprotein gIII) has been inserted into the baculovirus transfer vector pAcYM1S derived from the nuclear polyhedrosis virus of Autographa californica (AcNPV). A Spodoptera frugiperda cell line, SF21AE, was efficiently co-transfected with the transfer vector containing the gIII gene ... More
Differential regulation of vascular endothelial growth factor expression by peroxisome proliferator-activated receptors in bladder cancer cells.
AuthorsFauconnet S, Lascombe I, Chabannes E, Adessi GL, Desvergne B, Wahli W, Bittard H,
JournalJ Biol Chem
PubMed ID11980898
'The growth of any solid tumor depends on angiogenesis. Vascular endothelial growth factor (VEGF) plays a prominent role in vesical tumor angiogenesis regulation. Previous studies have shown that the peroxisome proliferator-activated receptor gamma (PPARgamma) was involved in the angiogenesis process. Here, we report for the first time that in two ... More
Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.
Authors Allshire R C;
JournalProc Natl Acad Sci U S A
PubMed ID2349217
'The efficiency of transformation of Schizosaccharomyces pombe has been increased 10- to 50-fold over previously reported methods. By using 1 microgram of plasmid, 7.0 x 10(5) transformants are regularly obtained. This increased transformation efficiency is mainly due to the inclusion of the cationic liposome-forming reagent Lipofectin in the protocol. Various ... More
Differential regulation of vitronectin in mice and humans in vitro.
Authors Seiffert D; Curriden S A; Jenne D; Binder B R; Loskutoff D J;
JournalJ Biol Chem
PubMed ID8621404
'To define the cis-acting elements involved in the regulation of the murine vitronectin (Vn) gene in inflammation, the 5''-flanking region was isolated, fused to the luciferase reporter gene, and the basal and interleukin 6 (IL-6)-stimulated transcriptional activity was tested in transfection experiments using Hep3B cells. Treatment with IL-6 induced this ... More
Efficient transfection of chicken cells by lipofection, and introduction of transfected blastodermal cells into the embryo.
Authors Brazolot C L; Petitte J N; Etches R J; Verrinder Gibbins A M;
JournalMol Reprod Dev
PubMed ID1751034
'Chicken blastodermal cells (CBCs) and primary chicken fibroblasts (PCFs) have been lipofected with a variety of lacZ constructs encoding Escherichia coli beta-galactosidase (beta-gal). A reporter construct (phspPTlacZpA) containing a mouse heat-shock protein 68 gene (hsp 68) promoter was used to establish conditions for efficient lipofection. The construct, in circular or ... More
Establishment and characterization of a rat glomerular endothelial cell line.
'BACKGROUND: Primary cell cultures have been widely used for research purposes, but kidney glomerular and vascular endothelial cells and, particularly, stable endothelial cell lines from these have been difficult to obtain. EXPERIMENTAL DESIGN: We used electroporation and lipofectin transfection to introduce various transforming constructs, and direct infection with an oncogenic ... More
Characterization of immortalized mouse granulosa cell lines.
Authors Briers T W; van de Voorde A; Vanderstichele H;
JournalIn Vitro Cell Dev Biol Anim
PubMed ID8167900
'Cell cultures of primary mouse granulosa cells were transfected with a v-myc-containing plasmid, and the resulting stable cell lines were tested for their steroidogenic properties and physiologic status. Granulosa cells were obtained from 22-day-old NMRI mice injected with 8 IU pregnant mare serum gonadotropin i.p. 2 days earlier. In Passage ... More
Liposome mediated in vitro transfection of pancreatic islet cells.
Authors Welsh N; Oberg C; Hellerström C; Welsh M;
JournalBiomed Biochim Acta
PubMed ID2097988
'The aim of this study was to evaluate the suitability of the liposome technique for transfection of pancreatic islet cells in vitro. For this purpose, fetal islets were isolated and cultured free floating for two days after which they were further cultured, either intact or dispersed into islet cell suspensions, ... More
Herbimycin A induces the 20 S proteasome- and ubiquitin-dependent degradation of receptor tyrosine kinases.
Authors Sepp-Lorenzino L; Ma Z; Lebwohl D E; Vinitsky A; Rosen N;
JournalJ Biol Chem
PubMed ID7622464
'Herbimycin A is an ansamycin antibiotic isolated as an agent that reverses morphological transformation induced by v-src. Although herbimycin A is widely used as a tool for inhibiting multiple tyrosine protein kinases and tyrosine kinase-activated signal transduction, its mechanism of action is not well defined and includes a decrease in ... More
Manipulation of hyaluronan synthase expression in prostate adenocarcinoma cells alters pericellular matrix retention and adhesion to bone marrow endothelial cells.
Authors Simpson Melanie A; Wilson Christopher M; Furcht Leo T; Spicer Andrew P; Oegema Theodore R Jr; McCarthy James B;
JournalJ Biol Chem
PubMed ID11790779
'Prostate cancer metastasis to bone marrow involves initial adhesion of tumor cells to the bone marrow endothelium, followed by transmigration and proliferation within the marrow. Rapid, specific adhesion of highly metastatic prostate adenocarcinoma cells (PC3M-LN4) to bone marrow endothelial cell (BMEC) lines requires a pericellular hyaluronan (HA) matrix and correlates ... More
1-(5-Isoquinolinesulfonyl)-2-methylpiperazine induces apoptosis in human neuroblastoma cells, SH-SY5Y, through a p53-dependent pathway.
AuthorsRonca F, Chan SL, Yu VC
JournalJ Biol Chem
PubMed ID9020141
'We have studied the effect of 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), a protein kinase inhibitor, on the regulation of apoptosis in the human neuroblastoma cell line, SH-SY5Y. H-7 (20-100 microM) induced apoptosis in these cells characterized by DNA fragmentation and chromatin condensation. Immunoblot analyses were performed with specific antibody against BCL-2, BCL-XS/L, BAX, ... More
Regulation of the human P-selectin promoter by Bcl-3 and specific homodimeric members of the NF-kappa B/Rel family.
Authors Pan J; McEver R P;
JournalJ Biol Chem
PubMed ID7559449
'P-selectin, an adhesion receptor for leukocytes, is constitutively expressed by megakaryocytes and endothelial cells. Synthesis of P-selectin is also increased by some inflammatory mediators. We characterized a previously identified kappa B site (-218GGGGGTGACCCC-207) in the promoter of the human P-selectin gene. The kappa B site was unique in that it ... More
The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.
Authors Münger K; Phelps W C; Bubb V; Howley P M; Schlegel R;
JournalJ Virol
PubMed ID2476573
'The early human papillomavirus type 16 genes that directly participate in the in vitro transformation of primary human keratinocytes have been defined. In the context of the full viral genome, mutations in either the E6 or E7 open reading frame completely abrogated transformation of these cells. Mutations in the E1, ... More
Alzheimer's disease betaA4 protein release and amyloid precursor protein sorting are regulated by alternative splicing.
Authors Hartmann T; Bergsdorf C; Sandbrink R; Tienari P J; Multhaup G; Ida N; Bieger S; Dyrks T; Weidemann A; Masters C L; Beyreuther K;
JournalJ Biol Chem
PubMed ID8662794
'We show here that alternative splicing influences the polarized secretion of amyloid precursor protein (APP) as well as the release of its proteolytic 3-4-kDa fragments betaA4 and p3. In Madin-Darby canine kidney II cells stably transfected with various APP isoforms and APP mutants, APPsec was consistently secreted basolaterally. In contrast, ... More
The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P.
Authors Lenz O; ter Meulen J; Klenk H D; Seidah N G; Garten W;
JournalProc Natl Acad Sci U S A
PubMed ID11606739
'The surface glycoprotein of the Lassa virus, a member of the arenaviridae family, is synthesized as a 76-kDa precursor (GP-C) that is posttranslationally cleaved into an N-terminal 44-kDa subunit and a C-terminal membrane-anchored 36-kDa subunit. Cleavage occurs at the C-terminal end of the unusual recognition motif R-R-L-L. We show here ... More
Expression of the catalytic subunit of human DNA polymerase delta in mammalian cells using a vaccinia virus vector system.
Authors Zhang P; Frugulhetti I; Jiang Y; Holt G L; Condit R C; Lee M Y;
JournalJ Biol Chem
PubMed ID7713899
'The catalytic polypeptide of human DNA polymerase delta was overexpressed in BSC-40 cells (African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The recombinant human DNA polymerase delta was purified to homogeneity in two steps using an immunoaffinity column and a single-stranded DNA-cellulose column. Levels of expression were ... More
The proximal promoter of the human transglutaminase 3 gene. Stratified squamous epithelial-specific expression in cultured cells is mediated by binding of Sp1 and ets transcription factors to a proximal promoter element.
Authors Lee J H; Jang S I; Yang J M; Markova N G; Steinert P M;
JournalJ Biol Chem
PubMed ID8626812
'The transglutaminase 3 enzyme is expressed during the late stages of the terminal differentiation of the epidermis and in certain cell types of the hair follicle. The enzyme is thought to be critically involved in the cross-linking of structural proteins and in the formation of the cornified cell envelope, thereby ... More
The carboxyl terminus of GLUT4 contains a serine-leucine-leucine sequence that functions as a potent internalization motif in Chinese hamster ovary cells.
Authors Garippa R J; Johnson A; Park J; Petrush R L; McGraw T E;
JournalJ Biol Chem
PubMed ID8702815
'To characterize the trafficking motifs contained in the carboxyl terminus of GLUT4, a chimera (GTCTR) was constructed in which the carboxyl-terminal 30 amino acids of GLUT4 were substituted for the amino-terminal cytoplasmic domain of the transferrin receptor (TR). The endocytic behavior of this chimera was characterized in Chinese hamster ovary ... More
p62, a phosphotyrosine-independent ligand of the SH2 domain of p56lck, belongs to a new class of ubiquitin-binding proteins.
'p62 is a novel cellular protein which was initially identified as a phosphotyrosine-independent ligand of the SH2 domain of p56(lck). In the yeast two-hybrid system, p62 specifically interacted with ubiquitin in vivo. Furthermore, p62 bound to ubiquitin-conjugated Sepharose beads in vitro and was efficiently competed by soluble ubiquitin. The interaction ... More
Osmoregulation of natriuretic peptide receptor signaling in inner medullary collecting duct. A requirement for p38 MAPK.
Authors Chen Songcang; Gardner David G;
JournalJ Biol Chem
PubMed ID11744737
'In the inner medullary collecting duct of the terminal nephron, the type A natriuretic peptide receptor (NPR-A) plays a major role in determining urinary sodium content. This nephron segment, by virtue of its medullary location, is subject to very high levels of extracellular tonicity. We have examined the ability of ... More
Molecular requirements for the interaction of class II major histocompatibility complex molecules and invariant chain with calnexin.
Authors Arunachalam B; Cresswell P;
JournalJ Biol Chem
PubMed ID7852350
'Molecular chaperones are believed to retain misfolded and incompletely assembled oligomeric proteins in the endoplasmic reticulum (ER). Here, we have further analyzed the association of one such chaperone, calnexin, with human major histocompatibility complex class II alpha and beta subunits and the invariant chain. Calnexin associates with transport-competent invariant chain ... More
Increased efficiency of liposome-mediated transfection by volume reduction and centrifugation.
Authors Verma R S; Giannola D; Shlomchik W; Emerson S G;
JournalBiotechniques
PubMed ID9668974
'N/A'
Expression of the hepatitis C virus genome in rat liver after cationic liposome-mediated in vivo gene transfer.
AuthorsTakehara T, Hayashi N, Miyamoto Y, Yamamoto M, Mita E, Fusamoto H, Kamada T
JournalHepatology
PubMed ID7875673
'The lack of a small animal model of hepatitis C virus (HCV) infection has impeded elucidation of the pathogenesis of HCV. The aim of this study was to develop an HCV-expressing animal model by means of cationic liposome-mediated in vivo gene transfer. To examine the feasibility of this strategy, pActLacZ, ... More
Human tumor-derived genomic DNA transduced into a recipient cell induces tumor-specific immune responses ex vivo.
'This article describes a DNA-based vaccination strategy evaluated ex vivo with human cells. The vaccine was prepared by transferring tumor-derived genomic DNA to PCI-13 cells, a highly immunogenic tumor cell line (' ... More
Transforming growth factor-beta regulates collagen gel contraction by increasing alpha 2 beta 1 integrin expression in osteogenic cells.
AuthorsRiikonen T, Koivisto L, Vihinen P, Heino J
JournalJ Biol Chem
PubMed ID7529233
'The contraction of floating collagen gels is suggested to mimic the reorganization of collagenous matrix during development and tissue healing. Here, we have studied two osteogenic cell lines, namely MG-63 and HOS, and a chemically transformed subclone of HOS cells, HOS-MNNG. Transforming growth factor-beta (TGF-beta), a putative regulator of bone ... More
Cloning and analysis of the murine Fanconi anemia group C cDNA.
AuthorsWevrick R, Clarke CA, Buchwald M
JournalHum Mol Genet
PubMed ID7689006
'Fanconi anemia (FA) is one of a group of disorders characterized at the cellular level by a combination of hypersensitivity to DNA-damaging agents, chromosomal instability, and defective DNA repair. Clinical features of FA include pancytopenia, often accompanied by specific congenital malformations, and a predisposition to leukemia. Since the hematological manifestations ... More
Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene.
Authors Jang S I; Steinert P M; Markova N G;
JournalJ Biol Chem
PubMed ID8798649
'The human profilaggrin gene is expressed in the granular layer during the late stages of terminal differentiation of the epidermis. In in vitro transcription experiments we show that the abundance of the mRNA and the specificity of the expression are regulated primarily at the level of transcription. We found that ... More
Regulation of human involucrin promoter activity by POU domain proteins.
Authors Welter J F; Gali H; Crish J F; Eckert R L;
JournalJ Biol Chem
PubMed ID8663077
'POU domain transcription factors are expressed in the epidermis and are thought to be important regulators of keratinocyte gene expression. In the present article we demonstrate that POU transcription factors suppress transcription of the human involucrin (hINV) promoter. Cotransfection of pINV-2473, a construct containing 2473 base pairs of hINV upstream ... More
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Authors Felgner P L; Gadek T R; Holm M; Roman R; Chan H W; Wenz M; Northrop J P; Ringold G M; Danielsen M;
JournalProc Natl Acad Sci U S A
PubMed ID2823261
'A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, ... More
Signal transduction through a biomolecular receptor tyrosine protein kinase composed of a platelet-derived growth factor receptor-CD4 chimera and the nonreceptor tyrosine protein kinase Lck.
'We have generated a novel receptor tyrosine kinase by fusing the extracellular and transmembrane domain of the mouse platelet-derived growth factor receptor (PDGFR) to the cytoplasmic domain of CD4 and coexpressing the construct with the murine cytoplasmic tyrosine protein kinase p56lck. NMuMG cells, which are mouse mammary gland epithelial cells ... More
Protein kinase C isotypes in human erythroleukemia (K562) cell proliferation and differentiation. Evidence that beta II protein kinase C is required for proliferation.
AuthorsMurray NR, Baumgardner GP, Burns DJ, Fields AP
JournalJ Biol Chem
PubMed ID8340409
'The human erythroleukemia (K562) cell line undergoes megakaryocytic differentiation and cessation of proliferation when treated with phorbol myristate acetate (PMA). To investigate the role of individual protein kinase C (PKC) isotypes in these events, we have assessed PKC isotype expression during leukemic proliferation and PMA-induced differentiation. Immunoblot analysis using isotype-specific ... More
Localization and induced expression of fusion genes in the rat lung.
AuthorsHazinski TA, Ladd PA, DeMatteo CA
JournalAm J Respir Cell Mol Biol
PubMed ID1848084
'Liposome-mediated gene transfer is useful for DNA transfection into cells in culture. We wondered whether this method could be used to introduce new DNA into the intact lung. Fusion genes containing either the Rous sarcoma virus (RSV) promoter or the mouse mammary tumor virus (MMTV) promoter (which contains glucocorticoid response ... More
The promiscuous chemokine binding profile of the Duffy antigen/receptor for chemokines is primarily localized to sequences in the amino-terminal domain.
Authors Lu Z H; Wang Z X; Horuk R; Hesselgesser J; Lou Y C; Hadley T J; Peiper S C;
JournalJournal of Biological Chemistry
PubMed ID7592830
'The Duffy antigen (DARC) is a promiscuous chemokine receptor that also binds Plasmodium vivax. DARC belongs to a family of heptahelical chemokine receptors that includes specific (IL-8RA) and shared (IL-8RB) IL-8 receptors. Ligand binding specificity of IL-8 receptors was localized to the amino-terminal extracellular (E1) domain. To determine the basis ... More
Differential adenoassociated virus vector-driven expression of a neuropeptide Y gene in primary rat brain astroglial cultures after transfection with Sendai virosomes versus Lipofectin.
Authors de Fiebre C M; Wu P; Notabartolo D; Millard W J; Meyer E M;
JournalNeurochem Res
PubMed ID8065520
'The ability of Sendai virosomes or Lipofectin to introduce an AAV vector into primary rat brain astroglial cultures was characterized. The pJDT95npy vector was constructed by inserting rat NPY cDNA downstream from the indigenous AAV p5, p19 and p40 promoters in pJDT95. Lipofectin-mediated transfection with pJDT95npy (10 micrograms) resulted in ... More
The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells.
Authors Yang B; Brown D; Verkman A S;
JournalJ Biol Chem
PubMed ID8617713
'The mercurial insensitive water channel (MIWC, AQP-4) is a water-selective transporter expressed at the basolateral plasma membrane of principal cells in kidney collecting duct, airway epithelium, and gastric parietal cells, as well as in astrocytes and skeletal muscle plasmalemma. Because these sites correspond to membranes where orthogonal arrays of particles ... More
Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells.
Authors Tsai J C; Jain M; Hsieh C M; Lee W S; Yoshizumi M; Patterson C; Perrella M A; Cooke C; Wang H; Haber E; Schlegel R; Lee M E;
JournalJ Biol Chem
PubMed ID8631978
'Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA ... More
Cyclic AMP-dependent activation of Rap1b.
Authors Altschuler D L; Peterson S N; Ostrowski M C; Lapetina E G;
JournalJ Biol Chem
PubMed ID7737967
'Rap1 proteins belong to the Ras superfamily of small molecular weight GTP-binding proteins. Although Rap1 and Ras share approximately 50% overall amino acid sequence identity, the effector domains of the two proteins are identical, suggesting either similar or antagonistic signaling roles. Several pathways leading to Ras activation have been defined, ... More
Identification of potential tyrosine-containing endocytic motifs in the carboxyl-tail and seventh transmembrane domain of the neurokinin 1 receptor.
Authors Böhm S K; Khitin L M; Smeekens S P; Grady E F; Payan D G; Bunnett N W;
JournalJ Biol Chem
PubMed ID8999947
'Although agonist-induced endocytosis of G-protein-coupled receptors is critical for receptor desensitization and resensitization, receptor motifs that interact with the endocytic apparatus have not been adequately characterized. We examined the effects of mutating the rat neurokinin-1 receptor on endocytosis using 125I-substance P, fluorescent substance P, and receptor antibodies. Substance P induced ... More
Glycine-extended progastrin processing intermediates induce H+,K(+)-ATPase alpha-subunit gene expression through a novel receptor.
Authors Kaise M; Muraoka A; Seva C; Takeda H; Dickinson C J; Yamada T;
JournalJ Biol Chem
PubMed ID7744746
'Although plasma levels of G-Gly are equivalent to those of gastrin, G-Gly has essentially no acute effect on gastric acid secretion. However, we have observed that inhibition of gastrin amidation leads to increased plasma concentrations of G-Gly and enhanced gastric acid secretion. We hypothesized, therefore, that G-Gly might have a ... More
Sp1 is a component of the cytokine-inducible enhancer in the promoter of vascular cell adhesion molecule-1.
Authors Neish A S; Khachigian L M; Park A; Baichwal V R; Collins T;
JournalJ Biol Chem
PubMed ID7499419
'Transcription of the vascular cell adhesion molecule-1 (VCAM-1) gene in endothelial cells is induced by the inflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, and lipopolysaccharide. Previous studies demonstrated that the cytokine-response region in the VCAM1 promoter contains binding sites for the transcription factors nuclear factor-kappa B (NF-kappa B) and interferon ... More
Progression of coronary atherosclerosis is associated with a common genetic variant of the human stromelysin-1 promoter which results in reduced gene expression.
Authors Ye S; Eriksson P; Hamsten A; Kurkinen M; Humphries S E; Henney A M;
JournalJ Biol Chem
PubMed ID8662692
'There is a common polymorphism in the promoter sequence of the human stromelysin-1 gene, with one allele having a run of six adenosines (6A) and the other five adenosines (5A). We have previously reported, in a 3-year follow-up study of patients with coronary atherosclerosis, that those patients who are homozygous ... More
Recombinant domain III of perlecan promotes cell attachment through its RGDS sequence.
Authors Chakravarti S; Horchar T; Jefferson B; Laurie G W; Hassell J R;
JournalJ Biol Chem
PubMed ID7814401
'Perlecan has been previously been shown to support attachment of a wide variety of cells through interactions of its core protein with the cell surface. The core protein domains involved in cell adhesion are, however, unknown. The laminin-like domain III of murine perlecan contains an RGDS sequence and is a ... More
Antiviral activity and protection of cells against human immunodeficiency virus type-1 using an antisense oligodeoxyribonucleotide phosphorothioate complementary to the 5'-LTR region of the viral genome.
Authors Anazodo M I; Salomon H; Friesen A D; Wainberg M A; Wright J A;
JournalGene
PubMed ID8543166
'A COS-like monkey kidney cell line stably transfected with the plasmids pCMVgagpol-rre-r with the gag and pol genes, and pCMV rev with the rev gene of HIV-1 derived from the cDNA clone BH10, was used as a model for assessing the effectiveness of antisense (AS) constructs, A 20-mer oligodeoxyribonucleotide (oligo) ... More
Transepithelial transport of HIV-1 by M cells is receptor-mediated.
'Human colon carcinoma Caco-2 cell monolayers undergo conversion into cells that share morphological and functional features of M cells when allowed to interact with B lymphocytes. A lymphotropic (X4) HIV-1 strain crosses M cell monolayers and infects underlying CD4(+) target cells. Transport requires both lactosyl cerebroside and CXCR4 receptors, which ... More
Mechanisms of desensitization and resensitization of proteinase-activated receptor-2.
Authors Böhm S K; Khitin L M; Grady E F; Aponte G; Payan D G; Bunnett N W;
JournalJ Biol Chem
PubMed ID8703006
'Proteinase-activated receptor-2 (PAR-2) is a G-protein-coupled receptor that is expressed by intestinal epithelial cells, which are episodically exposed to pancreatic trypsin in the intestinal lumen. Trypsin cleaves PAR-2 to expose a tethered ligand, which irreversibly activates the receptor. Thus, PAR-2 may desensitize and resensitize by novel mechanisms. We examined these ... More
Inhibition of GFAP synthesis by antisense RNA in astrocytes.
Authors Yu A C; Lee Y L; Eng L F;
JournalJ Neurosci Res
PubMed ID1665525
'Glial fibrillary acidic protein (GFAP) accumulation is a prominent feature of astrocytic gliosis. The inhibition or delay in GFAP synthesis might delay scar formation resulting from an insult such as spinal cord injury or central nervous system (CNS) demyelination. The delay in the formation of a physical barrier might allow ... More
Induction of Galphaq-specific antisense RNA in vivo causes increased body mass and hyperadiposity.
Authors Galvin-Parton P A; Chen X; Moxham C M; Malbon C C;
JournalJ Biol Chem
PubMed ID9020153
'Transgenic BDF-1 mice harboring an inducible, tissue-specific transgene for RNA antisense to Galphaq provide a model in which to study a loss-of-function mutant of Galphaq in vivo. Galphaq deficiency induced in liver and white adipose tissue at birth produced increased body mass and hyperadiposity within 5 weeks of birth that ... More
Antisense gene inhibition by oligonucleotides containing C-5 propyne pyrimidines.
Authors Wagner R W; Matteucci M D; Lewis J G; Gutierrez A J; Moulds C; Froehler B C;
JournalScience
PubMed ID7684856
'Phosphorothioate oligodeoxynucleotides containing the C-5 propyne analogs of uridine and cytidine bind RNA with high affinity and are potent antisense inhibitors of gene expression. In a cellular assay, gene-specific antisense inhibition occurred at nanomolar concentrations of oligonucleotide, was dose-dependent and exquisitely sensitive to sequence mismatches, and was correlated with the ... More
The two nucleotide-binding domains of cystic fibrosis transmembrane conductance regulator (CFTR) have distinct functions in controlling channel activity.
Authors Carson M R; Travis S M; Welsh M J;
JournalJ Biol Chem
PubMed ID7530246
'The cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel contains two cytoplasmic nucleotide-binding domains (NBDs). After phosphorylation of the R domain, ATP interacts with the NBDs to regulate channel activity. To learn how the NBDs regulate channel function, we used the patch-clamp technique to study CFTR and variants which contained ... More
Transcriptional regulation of the transforming growth factor beta type II receptor gene by histone acetyltransferase and deacetylase is mediated by NF-Y in human breast cancer cells.
Authors Park Seok Hee; Lee Sae Ra; Kim Byung Chul; Cho Eun Ah; Patel Sejal P; Kang Hee-Bum; Sausville Edward A; Nakanishi Osamu; Trepel Jane B; Lee Byoung Ick; Kim Seong-Jin;
JournalJ Biol Chem
PubMed ID11744689
'Transcriptional repression of the transforming growth factor-beta (TGF-beta) type II receptor (TbetaRII) gene is one of several mechanisms leading to TGF-beta resistance. Previously, we have shown that MS-275, a synthetic inhibitor of histone deacetylase (HDAC), specifically induces the expression of the TbetaRII gene and restores the TGF-beta signaling in human ... More
Mutations in the B2 bradykinin receptor reveal a different pattern of contacts for peptidic agonists and peptidic antagonists.
'The B2 bradykinin receptor, a seven-helix transmembrane receptor, binds the inflammatory mediator bradykinin (BK) and the structurally related peptide antagonist HOE-140. The binding of HOE-140 and the binding of bradykinin are mutually exclusive and competitive. Fifty-four site-specific receptor mutations were made. BK''s affinity is reduced 2200-fold by F261A, 490-fold by ... More
Cleavage of the carboxyl tail from the G3 domain of aggrecan but not versican and identification of the amino acids involved in the degradation.
Authors Lee Vivian; Chen Liwen; Paiwand Frouz; Cao Liu; Wu Yaojiong; Inman Robert; Adams Mark E; Yang Burton B;
JournalJ Biol Chem
PubMed ID11932252
'Aggrecan, a major structural proteoglycan in cartilage, contains three globular domains, G1, G2, and G3, as well as sequences for glycosaminoglycan modification. A large number of proteases are implicated in aggrecan cleavage in normal metabolism, aging, and arthritis. These proteases are known to cleave at the IGD, KS, and CS ... More
The Role of Constant Region Carbohydrate in the Assembly and Secretion of Human IgD and IgA1.
Authors Gala Francoise A; Morrison Sherie L;
JournalJ Biol Chem
PubMed ID12023968
'Immunoglobulins are glycoproteins, containing N- linked carbohydrates in the heavy chain constant regions of all isotypes and O-linked carbohydrates in the hinge regions of human IgA1 and IgD. A previous study showed that IgD synthesized in the presence of tunicamycin and lacking the three N-linked glycans on the heavy chain ... More
'Connective tissue growth factor (CTGF) is abundantly expressed in the vascular smooth muscle cells (VSMC) of atherosclerotic lesions but not in normal vessels. CTGF is able to promote VSMC proliferation and migration and influences the composition of extracellular matrix. The mechanisms for controlling these events remain unclear. We studied the ... More
A novel nonviral cytoplasmic gene expression system and its implications in cancer gene therapy.
Authors Chen X; Li Y; Xiong K; Xie Y; Aizicovici S; Snodgrass R; Wagner T E; Platika D;
JournalCancer Gene Ther
PubMed ID8548582
'We recently have developed a unique cytoplasmic transient gene expression system based on cotransfection of target cells with bacteriophage T7 RNA polymerase (RNAP) and plasmid DNA vectors containing a T7 autogene. Because this T7 system is self-initiating, self-maintaining, and requires no cellular factors for transcription, it is therefore likely to ... More
Human T-cell lymphotropic virus type 1 tax inhibits transforming growth factor-beta signaling by blocking the association of Smad proteins with Smad-binding element.
Authors Lee Dug Keun; Kim Byung-Chul; Brady John N; Jeang Kuan-Teh; Kim Seong-Jin;
JournalJ Biol Chem
PubMed ID12097320
'The human T-cell lymphotropic virus type 1 (HTLV-1) oncoprotein Tax is implicated in various clinical manifestations associated with infection by HTLV-1, including an aggressive and fatal T-cell malignancy. Because many human HTLV-1-infected T-cell lines are resistant to the growth inhibitory activity of transforming growth factor beta (TGF-beta), we examined the ... More
Characterization of the IL-6 responsive elements in the gamma fibrinogen gene promoter.
Authors Zhang Z; Fuentes N L; Fuller G M;
JournalJ Biol Chem
PubMed ID7592638
'Fibrinogen, a hepatically derived class II acute phase protein, is the product of three separate genes, (A alpha, B beta, and gamma). The fibrinogen genes are expressed constitutively; however, their transcription can be significantly up-regulated by interleukin-6 (IL-6) and glucocorticoid. Inspection of the promoter region of the fibrinogen gamma gene ... More
Polyethyleneimine-mediated transfection to improve antisense activity of 3'-capped phosphodiester oligonucleotides.
AuthorsDheur S, Saison-Behmoaras TE
JournalMethods Enzymol
PubMed ID10595349
none
Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences.
Authors Ma M; Benimetskaya L; Lebedeva I; Dignam J; Takle G; Stein C A;
JournalNat Biotechnol
PubMed ID10625392
Most antisense oligonucleotide experiments are performed with molecules containing RNase H-competent backbones. However, RNase H may cleave nontargeted mRNAs bound to only partially complementary oligonucleotides. Decreasing such ... More
The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information.
AuthorsHicks SW, Machamer CE.
JournalJ Biol Chem
PubMed ID12130652
Golgin-160 is a member of the golgin family of Golgi-localized membrane proteins. The COOH-terminal two-thirds of golgin-160 is predicted to form a coiled-coil, with an NH(2)-terminal ... More
Heat shock-sensitive expression of calreticulin. In vitro and in vivo up-regulation.
Authors Conway E M; Liu L; Nowakowski B; Steiner-Mosonyi M; Ribeiro S P; Michalak M;
JournalJ Biol Chem
PubMed ID7622522
Calreticulin (CRT) is an ubiquitous, highly conserved, Ca(2+)-binding protein of the sarcoplasmic and endoplasmic reticulum. The precise function(s) of CRT is unknown. However, based on sequence analyses and observations that it may bind to steroid receptors and integrins and store Ca2+ within the cell, it has been postulated to play ... More
Lipofection does not require the removal of serum.
Authors Brunette E; Stribling R; Debs R;
JournalNucleic Acids Res
PubMed ID1549487
Researchers showed that Lipofectin, Transfectase and other cationic lipids produce transient CAT activity that is at least as high in the presence of serum (10%) as achieved w/ a totally serum free protocol (w/ MEL and CV-1 cells). Lipid/DNA complexes were formed in the absence of serum prior to ... More