Thermo Scientific™

Amino Acid Standard H

Catalog number:  20088

 Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce Amino Acid Standard H is a quantitative mixture of 18 amino acids, supplied at 2.5 µMol/mL each in 0.1N HCl, for use as a high-purity calibration standard for HPLC analysis of protein hydrolysates.

Features of Amino Acid Standard H:

Complete mixture—contains eighteen (18) amino acids for assessment of amino acid composition of peptide hydrolysates
Quantitative—contains exact amounts of each amino acid (2.5±0.1 µMol/mL, except cystine at 1.25±0.1 µMol/mL), enabling its use as a quantitation standard
Stable—dissolved in 0.1N hydrochloric acid and stabilized as 1 mL aliquots in sealed glass ampules
Standard H—designed for compositional amino acid analysis by HPLC methods of protein or peptide hydrolysates

This quantitative solution of eighteen purified amino acids in 0.1N HCl is an ideal reference standard for calibrating amino acid analyzers and assessing the composition of hydrolyzed peptides and proteins (hydrolysates). To permit standardization of microbiological and other assays, the mixture uses only the L-form of each amino acid. The molar concentration of each amino acid is verified by conventional amino acid analysis methods, making the mixture suitable as both a qualitative and quantitative standard. The solution is provided as stable 1 mL aliquots in sealed glass ampules.

Product Details:
When kept frozen, an unopened vial has an indefinite storage life. Once the seal is broken, the reagent has a maximum storage life of six months (store frozen between uses). To use the product, thaw a vial and shake well to ensure a homogeneous solution. Then dilute appropriately with suitable buffers to a concentration compatible with the full-scale sensitivity of your amino acid analyzer.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

For Research Use Only. Not for use in diagnostic procedures.


Product Size: 10 x 1 mL

Contents & storage

Store between -20°C and 8°C.


Manuals & protocols