Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates.
Features of Anti-c-Myc Agarose:
• Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity for immunoprecipitation • Scalable—available in 2 and 10 mL resin package sizes to allow for larger scale purifications or immunoprecipitations • Versatile—can be used in spin or gravity columns as well as in FPLC cartridges • Convenient—reagents to elute and detect c-Myc tagged fusion proteins are available separately
The anti-c-Myc antibody used to manufacture Pierce Anti-c-Myc Agarose is a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The support is Superflow 6 resin, a highly crosslinked 6% agarose resin. This anti-c-Myc affinity resin can be packed into gravity purification columns, spin purification columns or cartridges for FPLC instruments to purify c-Myc-fusion proteins expressed in bacterial or mammalian cells.
Pierce Anti-c-Myc Agarose consists of a highly specific monoclonal anti-c-Myc antibody that is covalently immobilized on a crosslinked 6% beaded agarose support. The product is supplied as a 25% slurry. Upon incubation with a sample, c-Myc tagged fusion protein is captured on the agarose beads. After simple washing steps, the tagged protein is easily eluted from the resin using 0.1M glycine (pH 2.0-2.8), 3M NaSCN or 50 mM NaOH depending on the downstream application of the purified protein. For elution of highly functional proteins, Pierce c-Myc-peptide can also be used to elute the c-Myc tagged protein. Anti-c-Myc antibody may be used to detect the presence of the tagged protein by Western blot.
In experiments with a c-Myc-tagged fusion protein (26 to 29kDa), the resin provided a binding capacity of 102 to 144nmol protein per mL of settled resin. The elution capacity was at least 18 to 19nmol per mL of settled resin using 0.1M glycine, pH 2.8. Binding and elution capacity will vary depending on the c-Myc-fusion protein and the method of elution.