Pierce™ c-Myc-Tag IP/Co-IP Kit

Catalog number: 23620

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments using a c-Myc-tagged protein as the bait.

The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression. This kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged bait protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.

Features of the c-Myc-Tag IP/Co-IP Kit:

Specific—the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection
High capacity—excellent results with as little as 1 µg of anti-c-Myc antibody in IP mode with the positive control lysate
Rigorous—kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls
Robust—the affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems
Convenient and easy—complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions

The c-Myc peptide (EQKLISEEDL) derived from the C-terminus region of human c-Myc protein is one of several fusion protein tags used for recombinant protein expression. Utilizing a specific, high-affinity immobilized antibody, c-Myc tagged fusion proteins can be quickly purified from bacterial and mammalian cell lysates as well as from the Pierce Human in vitro translation reactions. For co-immunoprecipitation reactions, simple wash steps allow enrichment and elution of specific protein interaction complexes into the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis.

Related Products
Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Immunoprecipitation (IP), Pull-Down (Tag-Based)
Assay Type: Affinity Purification
Product Size: 25 reactions
Target Specificity: Target-Specific
For Use With (Equipment): Microcentrifuge

Contents & storage

Sufficient For: 25 IP reactions, each using 5 µL resin (5 µg anti-c-Myc antibody)
• Anti-c-Myc Agarose Resin, 250 µL
• c-Myc-tagged Positive Control (Part No. 23633), 500 µL
• TBS Pack (makes 500 mL), 1 pack
• Elution Buffer (pH 2.0), 2 x 25 mL
• Conditioning Buffer (100X), 5 mL
• Non-Reducing Sample Buffer (5X), 5 mL
• Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes

Store the c-Myc-tagged Positive Control at -80°C. Store Application Set at 4°C.

Documents

Manuals & protocols

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