AminoLink™ Plus Micro Immobilization Kit, 0.1 mL

Catalog number: 20475

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

The Thermo Scientific AminoLink Plus Micro Immobilization Kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

Features of the AminoLink Plus Micro Immobilization Kit:

Optimized for small scale—ideal for coupling small amounts (25 to 250 µg) of purified antibodies, proteins and other amine-containing ligands for purification of specific antibodies from crude serum
High-performance affinity resin—covalent attachment of the protein to AminoLink Plus Coupling Resin results in a leach-resistant affinity support
Efficient coupling chemistry—immobilization efficiency greater than 80% (as measured with 4 hour coupling reaction using mammalian antibodies)
Fast and easy to use—perform wash and elution steps using a microcentrifuge
Reusable—use prepared peptide resin several times with no significant loss of capacity

The AminoLink Plus Micro Immobilization Kit is for immobilizing small amounts (25 to 100 µg) of purified antibody and other proteins onto Thermo Scientific AminoLink Plus Coupling Resin (crosslinked 4% beaded agarose) to create a small, reusable, microcentrifuge affinity column. The coupling and affinity purification procedures are optimized for small sample volumes (200 to 300 µL). Wash and elution steps are achieved rapidly and efficiently with the convenient microcentrifuge spin columns. Each kit contains sufficient reagents for 10 coupling reactions and 20 affinity purifications.

Related Products
AminoLink™ Plus Coupling Resin
AminoLink™ Plus Immobilization Kit, 2 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Spin Column, Kit, Microcentrifuge
Column Type: Affinity, Agarose Resin
Product Size: 10 columns
Immobilized Phase: Aldehyde

Contents & storage

Sufficient For: Immobilizing 25 to 100 µg of protein per column; Includes buffers for 20 affinity purifications
• AminoLink Plus Spin Columns, 0.1 mL, 10 columns
• PBS Pack (makes 500 mL), 1 pack
• Sodium Cyanoborohydride Solution, 5M, 0.5 mL
• Quenching Buffer, 60 mL
• Wash Solution, 25 mL
• Elution Buffer, 50 mL
• Collection Tubes, 200 tubes

Store at 4°C.

Documents

Manuals & protocols