AminoLink™ Plus Coupling Resin
Thermo Scientific™
AminoLink™ Plus Coupling Resin
Thermo Scientific AminoLink Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary aminesRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberFormulationQuantity
20501Aldehyde-activated 4% beaded agarose, slurried in water with sodium azide10 mL
20505Aldehyde-activated 4% beaded agarose, slurried in water with sodium azide50 mL
2 Options
Catalog number 20501
Price (USD)/ 10 mL
173.65
Online exclusive
177.00 
Save 3.35 (2%)
-
Add to cart
Formulation:
Aldehyde-activated 4% beaded agarose, slurried in water with sodium azide
Quantity:
10 mL
Request bulk or custom format
Price (USD)/ 10 mL
173.65
Online exclusive
177.00 
Save 3.35 (2%)
Add to cart
AminoLink™ Plus Coupling Resin
Catalog number20501
Price (USD)/ 10 mL
173.65
Online exclusive
177.00 
Save 3.35 (2%)
-
Add to cart
Thermo Scientific AminoLink Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Features of AminoLink Plus Coupling Resin:

AminoLink Plus CouplingResin—aldehyde-activated crosslinked 4% beaded agarose
Ideal for antibodies and other proteins— immobilize molecules via primary amines (-NH2)
Flexible coupling conditions—efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided
Stable, permanent immobilization—Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose—bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Versatile and reusable—prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

AminoLink Plus Resin is beaded agarose support that has been modified to contain aldehyde groups. Antibodies, other proteins, or any molecules with primary amines can be conjugated to the resin by an optimized form of reductive amination. Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.

Specifications:
Product Details:
The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride). The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20 mg of immobilized protein per milliliter of agarose resin.

Coupling efficiencies with AminoLink Plus Coupling Resin exceed those obtained with cyanogen bromide (CNBr) activated supports. Furthermore, AminoLink Plus Chemistry forms a bond with the amine-containing ligand that is more stable than with the CNBr method and is uncharged. These properties provide a more leak-resistant immobilization and lower nonspecific binding when used for affinity purification procedures.

Related Products
AminoLink™ Plus Immobilization Kit, 2 mL
AminoLink™ Plus Micro Immobilization Kit, 0.1 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormulationAldehyde-activated 4% beaded agarose, slurried in water with sodium azide
FormLiquid
Product LineAminoLink™
Quantity10 mL
TargetAntibody, Proteins through Primary Amine
TypeResin
Stationary PhaseAldehyde
FormatBottle
Column TypeAffinity, Agarose Resin
Unit Size10 mL
Contents & Storage
Store at 4°C.