Thermo Scientific™

DSG (disuccinimidyl glutarate)

Catalog number:  20593

Related applications:

Protein Labeling & Crosslinking

Back to top


Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of disuccinimidyl glutarate:

Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Membrane-permeable, allowing for intracellular crosslinking
• Can increase crosslinking efficiency compared to DSS in some applications

Product References
Crosslinker Application Guide -- search for recent literature references for this product

For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Homobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: NHS Ester
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Short

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.