Thermo Scientific™

DSS (disuccinimidyl suberate)

Catalog number: 21655
Thermo Scientific™

DSS (disuccinimidyl suberate)

Catalog number: 21655
Catalog Number
21655
Unit Size
50 mg
Price (USD)
Price: 85.50
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
10 x 2 mg
Price (USD)
Price: 90.25
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
1 g
Price (USD)
Price: 172.00
Your Price:
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-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2165550 mg
Price: 85.50
Your Price:
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A3926710 x 2 mg
Price: 90.25
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215551 g
Price: 172.00
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Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS is first dissolved in an organic solvent such as DMF or DMSO, then added to the aqueous crosslinking reaction. BS3, the water soluble analog of DSS is also available for applications that require a hydrophilic crosslinker (e.g. to effect cell-surface crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.

Features of disuccinimidyl suberate:

Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Membrane-permeable, allowing for intracellular crosslinking
• High purity, crystalline reagent can be used to create high-purity conjugates
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to BS3 (Sulfo-DSS)

Applications:
• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• 'Fix' protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Properties of DSS
• Alternative names: Disuccinimidyl suberate
• Molecular formula: C16H20N2O8
• Molecular weight: 368.35
• Spacer arm length: Å (8 atoms)
• CAS number: 68528-80-3
• Reactive groups: NHS esters, react with primary amines at pH 7.0–9.0

DSS Specifications
We manufacture DSS to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DSS crosslinker is tested to meet the following minimum specifications:
Identity: IR scan shows only peaks characteristic of the structure and functional groups of DSS
Purity: > 90% by quantitative NMR (the highest standard in crosslinking purity)
Solubility: > 9.2 mg/mL in DMF and DMSO, clear and colorless solution

Product References
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
BS3 (bis(sulfosuccinimidyl)suberate)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine-Amine
Cleavable
No
Description
DSS
Molecular Weight
368.35
PEGylated
No
Spacer Arm Length
11.4 Å
Cell-Permeable
Yes
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Homobifunctional
Reactive Moiety
NHS Ester
Spacer
Medium (10 to 30 Å)
Form
Powder
Quantity
50 mg
Solubility
DMF, DMSO
Format
Standard, Single use
Water-Soluble
No
Product Type
Crosslinker

Contents & Storage

Upon receipt store desiccated at 4°C.