Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.
Features of His Tag Protein Interaction Pull-Down Kit:
• 6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein • Complete kits—provide all components and detailed protocol for purifying protein:protein interactions • No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge) • Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples • Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types • Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding • Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin
• Discover a new protein:protein interaction from a cell lysate • Confirm a putative interaction from a cell lysate or with a previously purified protein • Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.