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View additional product information for EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format - FAQs (21331, A39258)
4 product FAQs found
Yes, we offer Sulfo-NHS-SS-Biotin (Cat. Nos. 21331, 21328). It has a disulfide bond in the spacer arm that can be cleaved with DTT or other reducing agents, thereby releasing the biotin tag from the previously labeled protein. This product is also available in kit format (Cat. Nos. 21945, 21445).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
EZ-Link Sulfo-NHS-SS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
We recommend using EZ-Link Sulfo-NHS-SS Biotin (Cat. No. 21331 or 21338).
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.