Thermo Scientific™

EZ-Link™ Pentylamine-Biotin

Catalog number: 21345
Thermo Scientific™

EZ-Link™ Pentylamine-Biotin

Catalog number: 21345
Catalog Number
21345
Unit Size
50 mg
Price (USD)
Price: 300.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2134550 mg
Price: 300.00
Your Price:
-
Product Overview
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Thermo Scientific EZ-Link Pentylamine-Biotin is biotin that has been conjugated to pentylenediamine to add a short, amine-terminated spacer to its valeric acid group, providing a simple amine-modified compound for different applications.

Features of EZ-Link Pentylamine-Biotin:

Biotinylation—label molecules and surfaces for assay or affinity purification methods involving avidin or streptavidin probes and resins
Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
Medium length—spacer arm (total length added to target) is 18.9 angstroms, representing a 6-atom extension of the native biotin valeric acid (and its conversion to a primary amine)

Pentylamine Biotin is a compound formed by modification of the valeric acid side chain of biotin with pentane-1,5-diamine. The compound contains terminal primary amine (-NH2), which provides a functional handle for derivatization or conjugation to proteins, surfaces and other molecules. Carbodiimide (EDC) and NHS-ester crosslinker chemistries are most often utilized for covalent modifications involving amino-biotin molecules. The compound is also useful as an amine-alternative to native (carboxylate) biotin for assays and methods that require a biotin standard or blocking step.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).

Applications:
• Used for colorimetric assays for Factor XIII and cellular transglutaminase
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Pentylamine-Biotin
Quantity
50 mg
Product Line
EZ-Link™
Cell Permeability
Cell-Impermeant
Chemical Reactivity
Carboxylic Acid, Ketone, Aldehyde
Reactive Moiety
Amine
Solubility
Water
Label or Dye
Biotin
Label Type
Biotin & Analogs
Spacer
Short

Contents & Storage

Store at 4°C.