Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™
Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™
Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™
Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™
Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™
Citas y referencias (5)
Thermo Scientific™

Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™

Thermo Scientific Pierce Sulfo-NHS formato No-Weigh es un reactivo de modificación química para convertir grupos carboxilo en ésteres reactivos deMás información
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Número de catálogoCantidad
24510500 mg
A3926910 x 2 mg
245255 g
Número de catálogo 24510
Precio (MXN)
-
Cantidad:
500 mg
Pedido a granel o personalizado
Thermo Scientific Pierce Sulfo-NHS formato No-Weigh es un reactivo de modificación química para convertir grupos carboxilo en ésteres reactivos de N-hidroxisucinimida (NHS) para los métodos de bioconjugación, entrecruzamiento, marcaje e inmovilización.

Características de Sulfo-NHS:

• La eficiencia del acoplamiento mediado por 1-etil-3-(3-dimetilaminopropil)carbodiimida hidrocloruro (EDC) aumenta en la presencia de Sulfo-NHS
• Los ésteres reactivos en NHS o Sulfo-NHS se pueden crear con cualquier molécula que contenga carboxilo
• Los derivados Sulfo-NHS suelen ser solubles en agua (se pueden añadir directamente a las soluciones tampón) e impermeables a las membranas (se pueden utilizar para el marcaje de la superficie celular)
• El Sulfo-NHS cristalino de alta pureza se puede utilizar para crear derivados activados de alta calidad

Sulfo-NHS (N-hidroxisulfosucinimida) permite el control y la modificación de las reacciones de enlace de carbodiimida con activación de los carboxilos (—COOH) para la conjugación con aminas primarias (—NH2). Los derivados se sintetizan fácilmente mediante la mezcla de Sulfo-NHS con una molécula que contiene carboxilo y un agente deshidratante como la EDC (EDAC) carbodiimida. Este método es la base para la generación de muchos tipos de reactivos de marcaje de proteínas, incluidos colorantes fluorescente reactivos de aminas, marcaje de afinidad a biotina y compuestos de pegilación.

Aplicaciones:
• Mejore la eficacia de las reacciones de acoplamiento de EDC
• Convierta los carboxilos en ésteres Sulfo-NHS reactivos a aminas
• Entrecruce de forma más eficaz las proteínas con superficies o gránulos revestidos de carboxilo
• Active las nanopartículas con ésteres amino-reactivos de Sulfo-NHS

Especificaciones para Sulfo-NHS:
Fabricamos N-hidroxisulfosucinimida con las mejores especificaciones posibles para producir los bioconjugados más específicos, garantizar la integridad de los datos y proporcionarle el mayor grado de uniformidad. Cada lote de Sulfo-NHS se prueba para cumplir las siguientes especificaciones mínimas:

• Pureza: superior al 95 % mediante RMN cuantitativa (el estándar más elevado de pureza del entrecruzador);
la pureza media del lote es superior al 99 %
• Solubilidad: la muestra se disuelve a 2 mg/ml en agua desionizada para producir una solución transparente e incolora
• Identidad: los barridos de infrarrojos muestran solo las características de los picos de N-hidroxisulfosucinimida

Referencias de producto:
Guía de aplicación entrecruzada: búsqueda de referencias bibliográficas recientes para este producto

Productos relacionados
Pierce™ Premium Grade Sulfo-NHS (N-hidroxisulfosucinimida)
NHS (N-hidroxisucinimida)
EDC (1-etil-3-(3-dimetilaminopropil)carbodiimida clorhidrato)
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularNo
DescripciónSulfo-NHS
FormularioPolvo
Método de etiquetadoEtiqueta química
Peso molecular217,13
PegiladoNo
Línea de productosPierce
Cantidad500 mg
Fracción reactivaCarbodiimida
Condiciones de envíoAmbiente
SolubilidadAgua
Soluble en aguaSí
Reactividad químicaAmino-carboxilo
CleavableNo
Tipo de enlace cruzadoHeterobifuncionales
FormatoEstándar, de un solo uso, de calidad superior
Tipo de productoEntrecruzador
SeparadorCorto
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar a 4 °C.

Preguntas frecuentes

Can NHS and EDC stock solutions be made for long-term storage before use?

No. EDC is very unstable in aqueous environments and must be dissolved immediately before use. NHS and Sulfo-NHS are relatively stable in solution but best results are obtained when they are dissolved immediately before use. Store these compounds desiccated at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are there any substances that interfere with NHS reactions?

Yes. Except for the intended targets, reactions must not contain carboxyl or amine compounds. Thus, Tris, glycine, lysine, ethanolamine or other amine- containing buffers must be avoided.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between NHS and Sulfo-NHS?

Sulfo-NHS is the sulfonate sodium salt of NHS; it is water-soluble, but not membrane-permeable. NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical. Sulfo-NHS is chosen for its ability to confer better solubility to the activated compound and/or to control its membrane permeability.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How are NHS and Sulfo-NHS used?

These compounds are used to modify a carboxyl group to form an amine-reactive ester. This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat. No. 22980, 22981, 77149, A35391). EDC causes a dehydration reaction between the carboxyl and the NHS hydroxyl group, giving rise to an NHS-ester-activated molecule. The activated molecule can then be reacted spontaneously with a primary amine-containing molecule. Although the carboxyl-molecules can be made to react directly with amines using EDC, the reaction is much more efficient with NHS because a stable intermediate is created. In fact, molecules that are activated as NHS esters can be dried and stored for later reaction to amine-containing targets.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are NHS and Sulfo-NHS used for?

These compounds are used in conjunction with the crosslinker EDC (Cat. No. 22980, 22981, 77149, A35391) to synthesize amine-reactive labeling reagents, crosslinkers and conjugation compounds. Any compound containing a carboxylic acid (-COOH), such as a protein, or biotin or peptide, can be activated with NHS or Sulfo-NHS to form an NHS ester that will spontaneously react to form covalent amide linkages with proteins and other molecules that contain primary amines (-NH2).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Sulfo-NHS (N-hidroxisulfosuccinimida), formato No-Weigh™ FAQs

Citations & References (5)

Citations & References
Abstract
Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces.
Authors:Guha Thakurta S, Miller R, Subramanian A
Journal:J Biomater Appl
PubMed ID:20819918
'The response of biomaterial surfaces when exposed to blood is in part dependent upon the nature and composition of the adsorbed layer of proteins. Surfaces passivated with albumin have been shown to reduce platelet adhesion and activation. In an attempt to develop surfaces that can selectively and specifically bind albumin, ... More
Mouse monoclonal antibodies to anthrax edema factor protect against infection.
Authors:Leysath CE, Chen KH, Moayeri M, Crown D, Fattah R, Chen Z, Das SR, Purcell RH, Leppla SH
Journal:Infect Immun
PubMed ID:21911463
'Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the ... More
Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
Authors:Bergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A
Journal:J Biol Chem
PubMed ID:22692205
'The two major mammalian sialic acids are N-acetylneuraminic acid and N-glycolylneuraminic acid (Neu5Gc). The only known biosynthetic pathway generating Neu5Gc is the conversion of CMP-N-acetylneuraminic acid into CMP-Neu5Gc, which is catalyzed by the CMP-Neu5Ac hydroxylase enzyme. Given the irreversible nature of this reaction, there must be pathways for elimination or ... More
Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles.
Authors:Sperling RA, Parak WJ
Journal:Philos Trans A Math Phys Eng Sci
PubMed ID:20156828
Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the ... More
The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II.
Authors:Ido K, Kakiuchi S, Uno C, Nishimura T, Fukao Y, Noguchi T, Sato F, Ifuku K
Journal:J Biol Chem
PubMed ID:22707728
The PsbP protein regulates the binding properties of Ca(2+) and Cl(-), and stabilizes the Mn cluster of photosystem II (PSII); however, the binding site and topology in PSII have yet to be clarified. Here we report that the structure around His-144 and Asp-165 in PsbP, which is suggested to be ... More