Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
Features of Sulfo-NHS:
• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS • Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule • Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-impermable (can be used for cell surface labeling) • High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives
Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
Applications: • Improve efficiency of EDC coupling reactions • Convert carboxyls to amine-reactive Sulfo-NHS esters • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently • Activate nanoparticles with amine-reactive Sulfo-NHS esters
Specifications for Sulfo-NHS: We manufacture N-hydroxysulfosuccinimide to the highest possible specifications to produce the most specific bioconjugates, to ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-NHS is tested to meet the following minimum specifications:
• Purity—Greater than 95% by quantitative NMR (the highest standard for crosslinker purity); average lot purity is greater than 99% • Solubility—Sample dissolves at 2 mg/mL in deionized water to yield a clear, colorless solution • Identity—IR scan shows only peaks characteristic of N-hydroxysulfosuccinimide
Product References: Crosslinker Application Guide -- search for recent literature references for this product