Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Catalog number: PG82071

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade Sulfo-NHS:

Sulfo-NHS—use with EDC crosslinker to prepare amine-reactive NHS ester reagents
High quality—identity and purity confirmed by several tests, including quantitative NMR
Product integrity—enhanced level of testing and characterization compared to standard grade
Lot retention—ample supply of past lots retained to ensure future process testing
Change management—Change Control Notification (CCN) service
Consistent manufacture—batch-specific manufacturing documentation review

Applications:
• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive sulfo-NHS esters
• Membrane-impermable for cell surface labeling
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-NHS (N-hydroxysulfosuccinimide)
Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Spacer: Short
Solubility: Water
Product Size: 500 mg
Labeling Method: Chemical Labeling
Crosslinker Type: Heterobifunctional

Contents & storage

Upon receipt store at 4°C. Product is shipped at ambient temperature.

Documents

Manuals & protocols