Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.
Features of Premium Grade Sulfo-NHS:
• Sulfo-NHS—use with EDC crosslinker to prepare amine-reactive NHS ester reagents • High quality—identity and purity confirmed by several tests, including quantitative NMR • Product integrity—enhanced level of testing and characterization compared to standard grade • Lot retention—ample supply of past lots retained to ensure future process testing • Change management—Change Control Notification (CCN) service • Consistent manufacture—batch-specific manufacturing documentation review
Applications: • Improve efficiency of EDC coupling reactions • Convert carboxyls to amine-reactive sulfo-NHS esters • Membrane-impermable for cell surface labeling • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently • Activate nanoparticles with amine-reactive sulfo-NHS esters
Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
Product References: Crosslinker Application Guide -- search for recent literature references for this product