Thermo Scientific Pierce NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
Features of NHS:
• Efficiency of EDC-mediated coupling is increased in the presence of NHS
• Amine-reactive NHS esters can be made with any carboxyl-containing molecule
• NHS derivatives of typical small molecules (e.g., biotin) are uncharged, organic-soluble (dissolve first in DMSO) and cell membrane-permeable
• High-purity, crystalline NHS can be used to create high-quality activated derivatives
NHS (N-hydroxysuccinimide), the uncharged analog of Sulfo-NHS (N
-hydroxysulfosuccinimide), enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2).Product References:
Crosslinker Application Guide -- search for recent literature references for this productRelated ProductsSulfo-NHS (N-hydroxysulfosuccinimide)EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
For Research Use Only. Not for use in diagnostic procedures.