Thermo Scientific™

NHS (N-hydroxysuccinimide)

Catalog number:  24500

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.

Features of NHS:

• Efficiency of EDC-mediated coupling is increased in the presence of NHS
• Amine-reactive NHS esters can be made with any carboxyl-containing molecule
• NHS derivatives of typical small molecules (e.g., biotin) are uncharged, organic-soluble (dissolve first in DMSO) and cell membrane-permeable
• High-purity, crystalline NHS can be used to create high-quality activated derivatives

NHS (N-hydroxysuccinimide), the uncharged analog of Sulfo-NHS (N-hydroxysulfosuccinimide), enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2).

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-NHS (N-hydroxysulfosuccinimide)
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Carbodiimide
Solubility: Water
Spacer: Short

Contents & storage

Upon receipt store at 4°C.