Thermo Scientific Pierce NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
Features of NHS:
• Efficiency of EDC-mediated coupling is increased in the presence of NHS • Amine-reactive NHS esters can be made with any carboxyl-containing molecule • NHS derivatives of typical small molecules (e.g., biotin) are uncharged, organic-soluble (dissolve first in DMSO) and cell membrane-permeable • High-purity, crystalline NHS can be used to create high-quality activated derivatives
NHS (N-hydroxysuccinimide), the uncharged analog of Sulfo-NHS (N-hydroxysulfosuccinimide), enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2).
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