EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit - FAQs

View additional product information for EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit - FAQs (21435)

5 product FAQs found

In the EZ-Link Sulfo-NHS-LC-Biotinylation kit, what groups does Sulfo-NHS-LC-Biotin react with for biotinylation?

The NHS ester on this biotinylation reagent reacts with primary amines. In proteins and peptides, these occur at the amino terminus and the epsilon amine (side chain) of lysine residues.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do the EZ-Link Micro Biotinylation Kits differ from the standard biotinylation kits?

The standard biotinylation kits contain components for labeling 1-10 mg of protein, and the Micro Biotinylation Kits are structured for labeling 50-200 µg of protein in 200-700 mL. The Micro kits contain the biotinylation reagents in small, individual packages and do not include components to perform the HABA assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you provide the shelf-life for EZ-Link Sulfo-NHS-LC-Biotinylation Kit?

EZ-Link Sulfo-NHS-LC-Biotinylation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is there any advantage of choosing a Sulfo-NHS biotin with a longer spacer arm than EZ-Link Sulfo NHS Biotin?

Sometimes. The biotin has to insert 9 angstrom into the pocket of avidin, streptavidin or NeutrAvidin to bind. If steric hindrance may be a problem, using a biotinylation reagent with a longer spacer arm (Sulfo-NHS-LC Biotin or Sulfo-NHS-LC-LC Biotin) may result in greater purification or deteciton.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.