Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Features of PDPH :
• Reactive groups: pyridyldisulfide and hydrazide • Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups • Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable) • Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
Product References: Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.