Pierce™ 660nm Protein Assay Kit

Citations & References (13)

Thermo Scientific™

Pierce™ 660nm Protein Assay Kit

製品番号（カタログ番号）	数量
22662	450 mL

製品番号（カタログ番号） 22662

価格（JPY）

71,800

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450 mL

一括またはカスタム形式をリクエストする

Pierce 660 nmタンパク質アッセイは、すぐに使用可能な界面活性剤および還元剤に対応するアッセイ試薬で、総タンパク質濃度をタンパク質標準液と比較し、迅速に測定します。このアッセイはクマシー染色によるBradford法よりも直線性が高く、ほとんどの界面活性剤、還元剤、その他の一般的に使用される試薬の濃度よりも高濃度に適合します。

すべての利用可能なタンパク質アッセイ機能を見る›

660 nm タンパク質アッセイキットの機能：
•適合性—他の色素ベースアッセイよりも幅広い界面活性剤および還元剤に対応
• すぐに使用可能—1液タイプの混ぜて使用する簡単なプロトコルで試薬の調製が不要
• 線形性—Bradfordアッセイ法と比較して直線性の高い標準曲線が得られる
• アッセイのフォーマット—アッセイは、試験管またはマイクロプレートで実施可能
• サンプルのボリューム—マイクロプレートでわずか10 µL、試験管で100 µL
• 保管—室温保管

アクセサリのイオン界面活性剤適合性試薬（IDCR）ではより幅広い界面活性剤との適合性が得られます。これにより、ブロモフェノールブルーを含むLaemmli SDSサンプルバッファーを含むサンプルに適した唯一のタンパク質アッセイとなります。Pierce 660 nmタンパク質アッセイでは、BCAタンパク質アッセイなどの他のアッセイと比較して高水準のタンパク質間変動（37%）が認められますが、単純な1液タイプで幅広い物質に適合することから、多くのルーチンアプリケーションに対して660 nmアッセイがより使いやすいものになります。Pierce 660 nmタンパク質アッセイは、試験管またはマイクロプレートのいずれかのフォーマットで実施できます。

Pierce 660 nmアッセイのタンパク質検出方法
Pierce 660 nmタンパク質アッセイは、独自の色素金属複合体を酸性条件下でタンパク質に結合させることにより、色素の最大吸収量が変化し、これが660 nmで測定されます。色素-金属複合体は、赤みがかった茶色で、タンパク質が結合すると緑色に変化します。色の変化は、低いpHで色素が脱プロトン化することによって引き起こされますが、これはタンパク質中で正帯電したアミノ酸基との相互作用により促進されます。そのため、この色素は主に、ヒスチジン、アルギニン、リジンなどのタンパク質の塩基性残基と相互作用します。また、程度は低いもののチロシン、トリプトファン、フェニルアラニンなどとも相互作用します。

Bradford およびBCAタンパク質アッセイは互換性のない界面活性剤や還元剤が存在する場合でもアッセイによる発色は安定しており、タンパク質の濃度の広い範囲にわたって濃度の増加に比例して色素も増加します。

関連製品
Pierce 660 nmタンパク質アッセイ用試薬
Pierce 660 nmタンパク質アッセイ試薬用イオン界面活性剤適合性試薬

研究用途にのみご使用ください。診断目的には使用できません。

アッセイタンパク質定量アッセイ

概要Pierce 660 nm Protein Assay Kit

使用対象（アプリケーション）溶液ベースの検出、吸光度

使用対象 (装置)分光光度計、マイクロプレートリーダー

製品ラインPierce

製品タイプProtein Quantitation Assay

数量450 mL

特異性非標的特異的

対応可能対象300 Tube Assays or 3000 Microplate Assays

検出法比色法

Unit SizeEach

組成および保存条件

対応範囲：試験管アッセイ300回またはマイクロプレートアッセイ3,000回
• Pierce 660 nmタンパク質アッセイ試薬、450 mL
• アルブミン標準希釈済みセット、7×3.5 mL

アッセイ試薬は室温で保存します。希釈済みBSA標準液は4℃で保存します。

よくあるご質問（FAQ）

Which Thermo Scientific protein assay is the best or the most reliable?

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can I use the Thermo Scientific 660 nm Protein Assay to quantify peptides?

The Thermo Scientific 660 nm Protein Assay can quantify peptides that are at least 2,500 Da if their compositions include amino acid residues that react with the dye-metal reagent (i.e., histidine, arginine, tyrosine, tryptophan and phenylalanine). For peptides smaller than 2,500 Da, use the Fluoraldehyde Reagent Solution (Cat. No. 26025), which detects amino groups.

Other peptide quantitation assays we offer are Thermo Scientific Pierce Quantitative Colorimetric Peptide Assay (Cat. No. 23275) and Thermo Scientific Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the linear range of the Thermo Scientific 660 nm Protein Assay?

The assay's linear range is 25-2,000 µg/mL using the test tube procedure and 50-2,000 µg/mL using the microplate procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I measure the absorbance at a wavelength other than 660 nm when using the Thermo Scientific 660nm Protein Assay Kit?

If a 660nm filter is not available, the assay can be measured at wavelengths from 645 to 670nm; however, the assay linear range is 25-2,000 µg/mL and occurs only when the absorbance of the dye-protein complex is measured at 660 nm. Measuring the absorbance at wavelengths other than 660nm will result in a decrease of the assay's linear range and might increase the minimum detection level (i.e., decrease sensitivity).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What advantages does the Thermo Scientific 660nm Protein Assay have over other protein assays?

The Thermo Scientific 660 nm Protein Assay has the following advantages: • Room temperature stability of the assay reagent • A greater linear range than the coomassie-based Bradford assays • Compatibility with commonly used detergents and reducing agents • Compatibility with samples lysed in Laemmli sample buffer • Rapid mix-and-read protocol

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ 660nm Protein Assay Kit FAQs

引用および参考文献 (13)

引用および参考文献

Abstract

A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves.

Authors:Andersen ND, Srinivas S, Piñero G, Monje PV

Journal:Sci Rep

PubMed ID:27549422

'We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. This protocol introduces: (1) an efficient method for enzymatic ... More

Overexpression of the Sorghum bicolor SbCCoAOMT alters cell wall associated hydroxycinnamoyl groups.

Authors:Tetreault HM, Scully ED, Gries T, Palmer NA, Funnell-Harris DL, Baird L, Seravalli J, Dien BS, Sarath G, Clemente TE, Sattler SE

Journal:PLoS One

PubMed ID:30289910

'Sorghum (Sorghum bicolor) is a drought tolerant crop, which is being developed as a bioenergy feedstock. The monolignol biosynthesis pathway is a major focus for altering the abundance and composition of lignin. Caffeoyl coenzyme-A O-methyltransferase (CCoAOMT) is an S-adenosyl methionine (SAM)-dependent O-methyltransferase that methylates caffeoyl-CoA to generate feruloyl-CoA, an intermediate ... More

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.

Authors:Kamerkar S, LeBleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, Lee JJ, Kalluri R

Journal:Nature

PubMed ID:28607485

'The mutant form of the GTPase KRAS is a key driver of pancreatic cancer but remains a challenging therapeutic target. Exosomes are extracellular vesicles generated by all cells, and are naturally present in the blood. Here we show that enhanced retention of exosomes, compared to liposomes, in the circulation of ... More

Mechanisms of PKC-Mediated Enhancement of HIF-1a Activity and its Inhibition by Vitamin K2 in Hepatocellular Carcinoma Cells.

Authors:Xia J, Ozaki I, Matsuhashi S, Kuwashiro T, Takahashi H, Anzai K, Mizuta T

Journal:Int J Mol Sci

PubMed ID:30813635

'Hypoxia-inducible factor 1 (HIF-1) plays important roles in cancer cell biology. HIF-1a is reportedly activated by several factors, including protein kinase C (PKC), in addition to hypoxia. We investigated the role of PKC isoforms and the effects of vitamin K2 (VK2) in the activation process of HIF-1a. Human hepatocellular carcinoma ... More

Genetic resistance to DEHP-induced transgenerational endocrine disruption.

Authors:Stenz L, Rahban R, Prados J, Nef S, Paoloni-Giacobino A

Journal:PLoS One

PubMed ID:31181066

'Di(2-ethylhexyl)phthalate (DEHP) interferes with sex hormones signaling pathways (SHP). C57BL/6J mice prenatally exposed to 300 mg/kg/day DEHP develop a testicular dysgenesis syndrome (TDS) at adulthood, but similarly-exposed FVB/N mice are not affected. Here we aim to understand the reasons behind this drastic difference that should depend on the genome of ... More

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