Thermo Scientific™

Pierce™ NEM (N-ethylmaleimide)

Catalog number:  23030

 Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Features of N-Ethylmaleimide (NEM):

• Permanently block sulfhydryls to prevent disulfide bond formation.
• Monitor the reaction by measuring the decrease in absorbance at 300nm
• Block sulfhydryl-containing reagents that interfere in enzyme assays

N-Ethylmaleimide (NEM) is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.

Related Products
Pierce™ MMTS (methyl methanethiosulfonate)
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Thiol
Label or Dye: Unreactive Group
Product Line: Pierce™
Product Size: 25 g
Solubility: Water
Spacer: Mid-Length

Contents & storage

Upon receipt store at -20°C.


Manuals & protocols