Micro BCA™ Protein Assay Kit

Catalog number: 23235

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Micro BCA Protein Assay Kit is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Features of the Micro BCA Protein Assay Kit:

Sensitive—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
High linearity—linear working range for BSA equals 0.5 to 20 µg/mL
Colorimetric—measure with a standard spectrophotometer or plate reader (562nm)
Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
Convenient—microplate and cuvette protocols provided with the instructions
Stable—kit stable at room temperature; prepared working reagent stable for to 24 hours

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

Related Products
Micro BCA™ Reagent A (MA)
Micro BCA™ Reagent B (MB)
Micro BCA™ Reagent C (MC)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Absorbance, Solution-based Detection
Assay Type: Protein Quantitation Assay
Product Size: 500 mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader, Spectrophotometer

Contents & storage

Sufficient For: 480 tube assays or 3200 microplate assays
• Micro BCA Reagent A (MA), 240 mL
• Micro BCA Reagent B (MB), 240 mL
• Micro BCA Reagent C (MC), 12 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL

Store at room temperature.

Documents

Manuals & protocols