Kit de ensayo de proteínas Micro BCA™

El kit de ensayo de proteínas BCA Pierce Micro es una versión de tres componentes de nuestros reactivos BCA, optimizadosMás información

Número de catálogo	Cantidad
23235	500 ml

Número de catálogo 23235

Precio (MXN)

Cantidad:

500 ml

Pedido a granel o personalizado

El kit de ensayo de proteínas BCA Pierce Micro es una versión de tres componentes de nuestros reactivos BCA, optimizados para medir la concentración total de proteínas de soluciones de proteínas diluidas (de 0,5 a 20 µg/ml).

Compare todos los ensayos de proteínas BCA disponibles ›

Las características del kit de ensayo de proteínas BCA Micro incluyen:
• Sensibilidad: detecte con eficacia hasta 0,5 µg/ml (2 µg/ml en formato de microplacas).
• Intervalo de trabajo: intervalo de trabajo lineal para BSA de 0,5 a 20 µg/ml
• Colorimétrico: mida con un espectrofotómetro estándar o un lector de placas a 562 nm
• Compatibilidad: no se ve afectado por las concentraciones típicas de la mayoría de los detergentes iónicos y no iónicos
• Estabilidad: kit estable a temperatura ambiente; estabilidad del reactivo de 24 horas de trabajo

El kit de ensayo de proteínas BCA Micro es una versión especializada del popular ensayo de proteínas BCA Pierce para determinar la concentración de proteínas de muestras diluidas. La combinación de los tres reactivos de BCA Micro produce una solución de trabajo suficientemente concentrada para medir las proteínas cuando se mezclan con un volumen equivalente de muestra. El resultado es un ensayo para medir con precisión soluciones de proteínas de 0,5 a 20 µg/ml. El ensayo es excepcionalmente lineal y exhibe niveles muy bajos de variabilidad proteína a proteína.

Productos relacionados
Reactivo A Micro BCA (MA)
Reactivo B Micro BCA (MB)
Reactivo C Micro BCA (MC)
Rompedores de ampollas

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ensayoEnsayo de BCA

DescripciónKit de ensayo de proteínas BCA Pierce Micro

Para utilizar con (aplicación)Detección basada en soluciones, absorbancia

Para utilizar con (equipo)Espectrofotómetro, lector de microplacas

Línea de productosMicro BCA, Pierce

Tipo de productoEnsayo de cuantificación de proteínas

Cantidad500 ml

EspecificidadSin especificidad

Suficiente para480 ensayos en tubo o 3200 ensayos en microplaca

Método de detecciónColorimétrico

Unit SizeEach

Contenido y almacenamiento

Suficiente para: 480 ensayos de tubos o 3200 ensayos de microplacas
• Reactivo A Micro BCA (MA), 240 ml
• Reactivo B Micro BCA (MB), 240 ml
• Reactivo C Micro BCA (MC), 12 ml
• Ampollas estándar de albúmina, 2 mg/ml, 10 x 1 ml

Almacenar a temperatura ambiente.

Preguntas frecuentes

Which Thermo Scientific protein assay is the best or the most reliable?

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the sample to working reagent ratio when using the BCA Protein Assay Kit?

For the Microplate Protocol the sample to working reagent (WR) ratio is 1:8 (25 µl sample plus 200 µl WR). If the sample amount is limited, a 1:20 ratio may be used (10 µl sample); in this case, however, the detection range of the assay will be limited to 125-2,000 µg/ml. For the Test Tube Procedure (Standard or Enhanced Protocols) the ratio is 1:20 (0.1 ml sample plus 2.0 ml WR). In the Micro BCA Assay Kit (for both Microplate and Test Tube Procedures), the ratio is 1:1 (1 part sample plus 1 part WR).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

I ordered the Micro BCA Protein Assay Kit (Cat. No. 23235). However, I have limited sample volumes. Can I use the Nanodrop to measure my samples using this kit?

For performing the Micro BCA Protein Assay Kit (Cat. No. 23235) in tubes and plates, the recommended sample volumes are 1 mL and 150 µL, respectively. If you have a limited amount of sample volume, you can perform the assay using a NanoDrop, which only requires 10 µL of sample and 10 µL of the Micro BCA Working Reagent (WR). Please refer to the Protocol for BCA Protein Assay Using a NanoDrop 2000/2000c Spectrophotometer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What BCA protein assay kits do you recommend for NanoDrop 2000/2000c Spectrophotometers (Cat. Nos. ND-2000, ND-2000C)?

NanoDrop 2000/2000c Spectrophotometers (Cat. Nos. ND-2000, ND-2000C) are compatible with most commercial BCA protein assay kits. We offer a number of BCA protein assay kits, including, but not limited to, the BCA Protein Assay Kit (Cat. No. J63283.QA), Micro BCA Protein Assay Kit (Cat. No. 23235, and Pierce BCA Protein Assay Kit (Cat. No 23225, 23227).

To identify, compare, and choose the best protein assay for your sample type and application, you can use our interactive Protein Assay Selection Guide.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you provide the shelf-life for the Pierce Micro BCA Protein Assay Kit?

The Pierce Micro BCA Protein Assay Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Kit de ensayo de proteínas Micro BCA™ FAQs

Citations & References (1)

Citations & References

Abstract

Measurement of protein using bicinchoninic acid.

Authors:Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC

Journal:Anal Biochem

PubMed ID:3843705

Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The ... More