Easy-Titer™ Human IgG (H+L) Assay Kit - FAQs

No. The level of cross-reaction is neglible for common mixtures that we have tested.

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No, these assay use beads with target-specific antibodies, so off-target immunoglobulins have little or no effect on assay performance.

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Make sure that you are mixing the samples vigorously. We set our shaker near the highest setting for this step. For best results, the beads must be fully dispersed.

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The assay method requires many-fold dilution of sample (so that the target immunoglobulin is at 5-500 ng/mL). To obtain accurate results, this dilution must be done carefully. Use properly matched pipette sizes (e.g., do not use a 200 µl pipette to measure 10 µl) and make the dilution in two or three steps (e.g., 10-fold times 10-fold)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

No. Any standard, clear 96-well microplate can be used for this assay method.

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Yes. Samples are diluted at least 20-fold (usually much more) in the assay dilution buffer, rendering them fully compatible with the bead agglutination method.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.