Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes

Catalog number: 24580

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

The Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membrane after transfer from polyacrylamide gels.

Features of the Reversible Protein Stain Kit for Nitrocellulose Membranes:

Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
Rapid—stain in less than 5 minutes; completely erase and reverse staining in less than 15 minutes
Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on nitrocellulose membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for nitrocellulose membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

Related Products
Pierce™ Reversible Protein Stain Kit for PVDF Membranes
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Molecule: Protein
Label or Dye: Proprietary Mix
Detection Method: Colorimetric
Detection Location: In-Blot Detection
Product Size: 1.5 L

Contents & storage

Sufficient For: 10 nitrocellulose membranes (8 cm x 8 cm) from mini gels (SDS-PAGE)
• Reversible Stain, 250 mL
• Destain, 2 x 500 mL
• Stain Eraser, 250 mL

Store at room temperature

Documents

Manuals & protocols