GelCode™ Blue Safe Protein Stain

Catalog number: 24594

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

Thermo Scientific GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping costs.

Features of GelCode Blue Safe Protein Stain:

Sensitive and fast—detect down to 9 ng of protein per band in 15 minutes
Safe—non-corrosive to skin, non-flammable and safe to ship and store until activated
Convenient—water washes only; no acid-fixative or methanol destain solutions required
Versatile—suitable for staining 1D and 2D polyacrylamide gels, nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
Compatible—useful for qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
Easy to use—add and dissolve activator crystals before first use, then simply add reagent to stain gels
Stable—store non-activated or activated stain at room temperature for up to one year

GelCode Blue Safe Protein Stain is a coomassie G-250 dye-based reagent for staining protein on polyacrylamide gels and membranes. GelCode Blue Safe Stain provides considerable sensitivity, allowing for the detection down to 9 ng of protein. This protein-specific stain allows bands to be viewed directly on a gel or membrane during staining and is compatible with mass spectrometry applications. The staining process is simple and flexible, usually requiring a 15-minute incubation of the gel or sample with the GelCode Blue Safe Protein Stain, followed by a simple water wash to yield blue protein bands and a clear background.

Because the supplied Activator Crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements.

For proper protein staining, the Activator Crystals must be dissolved in the staining reagent before use. Add the entire contents of the Activator Crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for 1 minute, and then allow it to remain at room temperature for 5 minutes before use. Store the activated stain at room temperature.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Molecule: Protein
Label or Dye: Coomassie
Detection Method: Colorimetric
Detection Location: In-Gel Detection, In-Blot Detection
Product Size: 1 L

Contents & storage

Store at room temperature.

Documents

Manuals & protocols