Search

  • Fale Conosco
  • Pedido rápido

Invitrogen

Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience™

7 Published Figures
9 References
View all (63) STAT5 alpha antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

25-9010-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 9 publications 9 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SRBCZX

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573533

Product Specific Information

Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.

Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.

Applications Reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein; lacks exon 18; mammary gland factor STAT5A; STAT; STAT5B; transcription factor; unnamed protein product

View more View less

Gene Aliases: MGF; STAT5

View more View less

UniProt ID: (Human) P42229, (Mouse) P42230

View more View less

Entrez Gene ID: (Human) 6776, (Mouse) 20850

View more View less

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphate ion binding sequence-specific DNA binding DNA-binding transcription factor binding promoter-specific chromatin binding double-stranded DNA binding
Process(es)
luteinization natural killer cell differentiation positive regulation of endothelial cell proliferation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter defense response signal transduction JAK-STAT cascade female pregnancy lactation positive regulation of cell proliferation gene expression peptidyl-tyrosine phosphorylation regulation of steroid metabolic process cytokine-mediated signaling pathway taurine metabolic process lipid storage regulation of cell adhesion eosinophil differentiation regulation of epithelial cell differentiation mammary gland development positive regulation of interleukin-2 production positive regulation of natural killer cell differentiation negative regulation of mast cell apoptotic process T cell differentiation in thymus interleukin-15-mediated signaling pathway interleukin-4-mediated signaling pathway reelin-mediated signaling pathway interleukin-5-mediated signaling pathway interleukin-2-mediated signaling pathway interleukin-7-mediated signaling pathway interleukin-9-mediated signaling pathway interleukin-3-mediated signaling pathway thrombopoietin-mediated signaling pathway regulation of multicellular organism growth positive regulation of multicellular organism growth positive regulation of activated T cell proliferation regulation of cell proliferation T cell homeostasis negative regulation of apoptotic process response to peptide hormone positive regulation of blood vessel endothelial cell migration positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation negative regulation of erythrocyte differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of natural killer cell mediated cytotoxicity development of secondary female sexual characteristics development of secondary male sexual characteristics Peyer's patch development positive regulation of inflammatory response mammary gland involution positive regulation of mast cell differentiation JAK-STAT cascade involved in growth hormone signaling pathway prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development mammary gland epithelium development positive regulation of mast cell proliferation cellular response to cytokine stimulus cellular response to granulocyte macrophage colony-stimulating factor stimulus negative regulation of T-helper 17 cell lineage commitment regulation of hemopoiesis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support