Thermo Scientific™

Pierce™ Traut's Reagent (2-iminothiolane)

Catalog number:  26101

 Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce Traut's Reagent (2-iminothiolane) is a small thiolation compound that reacts with primary amines (e.g., lysine side chains) to add a small spacer arm (8.1 angstroms) terminated by a free sulfhydryl group.

Features of Thermo Scientific Pierce Traut's Reagent (2-iminothiolane):

Simple—one-step conversion of amines to sulfhydryls
Specific—selective and spontaneous modification of primary amines at pH 7-10
• Preserves positive charge to maintain protein solubility

Traut's Reagent (2-iminothiolane or 2-IT) is a cyclic thioimidate compound for thiolation (sulfhydryl addition). Traut's Reagent reacts with primary amines (—NH2) to introduce sulfhydryl (—SH) groups while maintaining charge properties similar to the original amino group. Once added, sulfhydryl groups can be specifically targeted for reaction in a variety of useful labeling, crosslinking and immobilization procedures.

Flexible—incorporates a five-atom spacer arm to reduce steric hindrance
Soluble—preserves the original positive charge to preserve protein solubility

Related Products
Pierce™ SATA
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Amine
Label or Dye: Sulfhydryl Group
Product Line: Pierce™
Product Size: 500 mg
Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Spacer: Short

Contents & storage

Storage: Upon receipt store product at 4°C with desiccant. Product shipped at ambient temperature


Manuals & protocols