Thermo Scientific™

Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Catalog number:  26102

Related applications:

Protein Labeling & Crosslinking

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Description

Thermo Scientific Pierce SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.

Features of SATA:

• Adds a protected sulfhydryl that can be deprotected by hydroxylamine
• Allows long-term storage of the sulfhydryl-modified molecule
• Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
• Reacts with primary amines (e.g., lysine residues proteins) to form stable amide bonds
• Preserves protein activity with its mild, non-denaturing reaction conditions

SATA (N-succinimidyl S-acetylthioacetate) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form. The modified molecule can be stored indefinitely and treated with hydroxylamine to expose the labile sulfhydryl group when needed for conjugation reactions. SATA contains an N-hydroxysuccinimide (NHS) ester, which forms a stable, covalent amide bond with primary amines (i.e., lysine residues and the amino termini of proteins) and releases NHS as a by-product. De-protection (deacylation) to generate a free sulfhydryl is accomplished using hydroxylamine-HCl.

Sulfhydryl groups present on proteins, peptides and other compounds are important in protein chemistry/modification reactions. Frequently, thiols are unavailable or absent within the molecules of interest. Several reagents and techniques are available for introducing sulfhydryl groups or disulfides into proteins and peptides, including Traut's Reagent, variants of SPDP, and variants of SATA.

Related Products
Pierce™ Sulfhydryl Addition Kit
Pierce™ SATP
Pierce™ SAT(PEG)4
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label Type: Conjugating Groups
Label or Dye: Sulfhydryl Group
Product Line: Pierce™
Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Spacer: Short
Chemical Reactivity: Amine

Contents & storage

Upon receipt store desiccated at -20°C. Product is shipped at ambient temperature.