Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation.
Features of Hydroxylamine-HCl:
• Quench amine-labeling or crosslinking reactions (e.g., with NHS esters) • Expose protected sulfhydryl groups on SATA-modified molecules • Cleave carbonyl-containing crosslinkers such as EGS and Sulfo-EGS
Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine·HCl.
For Research Use Only. Not for use in diagnostic procedures.