Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Catalog number:  21565

Related applications:

Protein Labeling & Crosslinking

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Description

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EGS (ethylene glycol bis(succinimidyl succinate))
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Pierce™
Crosslinker Type: Homobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: NHS Ester
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Cleavable, Long

Contents & storage

Store at -20°C. Shipped at ambient temperature.