Thermo Scientific™

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Catalog number:  21566

Related applications:

Protein Labeling & Crosslinking

Back to top

Description

Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of Sulfo-EGS:

Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Water-soluble; compare to EGS
• Membrane-impermeable, allowing for cell surface labeling
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Pierce™
Crosslinker Type: Homobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Sulfo-NHS Ester
Solubility: Water
Spacer: Cleavable, Long

Contents & storage

Upon receipt store desiccated at -20°C. Product is shipped at ambient temperature.