Thermo Scientific™

Pierce™ Sulfhydryl Addition Kit

Catalog number: 23460
Thermo Scientific™

Pierce™ Sulfhydryl Addition Kit

Catalog number: 23460
Catalog Number
23460
Unit Size
1 reaction
Price (USD)
Price: 335.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
234601 reaction
Price: 335.00
Your Price:
***
Product Overview
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The Thermo Scientific Pierce Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups. SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.

Features of SATA:

• Adds a protected sulfhydryl that can be deprotected by hydroxylamine
• Allows long-term storage of the sulfhydryl-modified molecule
• Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
• Reacts with primary amines (e.g., lysine residues proteins) to form stable amide bonds
• Preserves protein activity with its mild, non-denaturing reaction conditions

SATA (N-succinimidyl S-acetylthioacetate) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form. The modified molecule can be stored indefinitely and treated with hydroxylamine to expose the labile sulfhydryl group when needed for conjugation reactions. SATA contains an N-hydroxysuccinimide (NHS) ester, which forms a stable, covalent amide bond with primary amines (i.e., lysine residues and the amino termini of proteins) and releases NHS as a by-product. De-protection (deacylation) to generate a free sulfhydryl is accomplished using hydroxylamine-HCl.

Sulfhydryl groups present on proteins, peptides and other compounds are important in protein chemistry/modification reactions. Frequently, thiols are unavailable or absent within the molecules of interest. Several reagents and techniques are available for introducing sulfhydryl groups or disulfides into proteins and peptides, including Traut's Reagent, variants of SPDP, and variants of SATA.

Related Products
Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Pierce™ SATP
Pierce™ SAT(PEG)4
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine
Label or Dye
Sulfhydryl Group
Label Type
Conjugating Groups
Product Line
Pierce™
Crosslinker Type
Heterobifunctional
Reactive Moiety
Active Ester, Succinimidyl Ester, NHS Ester
Spacer
Short
Form
Powder
Reagent Type
SATA
Quantity
1 Reaction
Product Type
Crosslinker
Solubility
DMF, DMSO

Contents & Storage

Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein
• SATA, 2 mg
• Hydroxylamine·HCl, 5 mg
• Conjugation Buffer Stock (10X), 20 mL
• PBS pack (makes 500 mL), 1 pack
• Dimethylformamide (DMF), 1 mL
• Dextran Desalting Column, 1 x 5 mL
• Column Extender, 1
• Ellman's Reagent (DTNB), 2 mg
• Cysteine·HCl, 20 mg

Store at 4°C.