For the Pierce Classic IP Kit protocol, Step B says to add 80 µL of Control Agarose Resin slurry into a spin column for 1 mg lysate. Does the 1 mg of lysate refer to the amount of protein in the lysate?
The "1 mg of lysate" mentioned in Step B of the user manual refers to the total amount of protein in the lysate. The binding capacity of the Control Agarose Resin slurry is 1 mg of lysate (protein) per 80 µl (40 µl of settled resin) of resin slurry.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.