Protein immobilization chemistry of NHS-activated agarose
Human IgG (25mg) was immobilized to 1mL of Pierce NHS-Activated Agarose. The affinity resin was used to purify secreted recombinant Protein G from <em>E. coli</em>. A final yield of 25mg of protein was obtained. Lane 1: bacterial pellet, Lane 2: MW marker, Lane 3: culture supernatant, Lane 4: flow-through, Lane 5-12: elutions, Lane 13: boiled resin following elutions.
NHS agarose is effective for immunoprecipitation of MAP kinase from HeLa cell lysate
Superior coupling capacity with NHS-Activated Agarose
NHS-Activated Agarose more effectively couples dilute samples than competing products
|Column Type:||Agarose Resin, Affinity|
|Stationary Phase:||NHS Ester|