Thermo Scientific™

Spectra™ Multicolor High Range Protein Ladder

Catalog number:  26625

 Related applications:

Protein Gel Electrophoresis

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Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300kDa) for use as size standards for high-MW proteins in gel electrophoresis and Western blotting.

Features of Spectra Multicolor High Range Protein Ladder:

Size range—eight proteins spanning 40 to 300kDa
Multicolor—three different colors for unambiguous band-size assignment
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible—colored bands transfer to membranes for Western blotting

• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

• Monitoring of protein migration during SDS-PAGE
• Verifying Western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots
• Locating a protein of interest for excision from an unstained preparative gel

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.
For Research Use Only. Not for use in diagnostic procedures.


Stain Type: Prestained
System: SDS-PAGE
Product Line: Spectra™
Product Size: 2 x 250 µL

Contents & storage

Upon receipt store at -20°C. Product is shipped with an ice pack.


Manuals & protocols