Thermo Scientific™

Spectra™ Multicolor High Range Protein Ladder

Catalog number:  26625

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
Number of Markers: 8
Product Line: Spectra™
Recommended Applications: Tris-acetate gels
Size Range: 40 to 300 kDa
System Type: SDS-PAGE
Molecular Weight: 300, 250, 180, 130, 100, 70, 50, 40 kDa
Stain Type: 3 colors: Blue, Orange, Green
Quantity: 2 x 250 mL

Contents & storage

Contents: two vials of 250 µL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C