PageRuler™ Prestained NIR Protein Ladder

Catalog number: 26635

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

Features of PageRuler Prestained NIR Protein Ladder:

Size range—10 proteins spanning 11 to 250kDa
Fluorescent—detect using 670nm red laser or 700nm channels with fluorescence imagers
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent sizing for near-infrared visualization of protein gels and blots
Reference band—55kDa band has greater intensity for easy orientation
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Applications:
• Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers

Requires:
Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: SDS-PAGE
Stain Type: Fluorescent
Product Size: 2 x 250 µL

Contents & storage

Upon receipt store at -20°C. Product is shipped with an ice pack.

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Manuals & protocols

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