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Pierce™ Biotin Quantitation Kit
Pierce™ Biotin Quantitation Kit
Citations & References (10)
Thermo Scientific™

Pierce™ Biotin Quantitation Kit

Thermo Scientific Pierceビオチン定量キットは、HABA試薬およびビオチン化タンパク質標準の分割量が含まれており、標識抗体やその他のタンパク質のビオチン化レベルを比色定量するのに便利です。ビオチン定量キットの特長：•比色定量 – 500 nmで測定するように設定されたプレートリーダーまたは分光光度計が必要• 信頼性詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
2800512 Tests
製品番号（カタログ番号） 28005
価格（JPY）
55,400
Each
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数量:
12 Tests
一括またはカスタム形式をリクエストする
Thermo Scientific Pierceビオチン定量キットは、HABA試薬およびビオチン化タンパク質標準の分割量が含まれており、標識抗体やその他のタンパク質のビオチン化レベルを比色定量するのに便利です。

ビオチン定量キットの特長：

•比色定量 – 500 nmで測定するように設定されたプレートリーダーまたは分光光度計が必要
• 信頼性 – 実績豊富で特徴が明確なビオチン化レベル測定手法
• 利便性 – キットはアッセイを簡単に実行するために不可欠な試薬と標準を提供
• 柔軟性 – 分光光度計キュベットまたは96ウェルマイクロプレートを備えた標準プレートリーダーに適応可能
• オプション – 取扱説明書に概説されている手順を使用し、吸光係数に基づいて吸光度値から直接結果を計算
• 堅牢 – 供給されるHABA-アビジン複合体は広範囲のpHおよび塩濃度で使用可能

HABA色素（4'-ヒドロキシアゾベンゼン-2-カルボン酸）は溶液中のタンパク質に対するビオチンのモル対モル比を迅速に推定できる試薬です。Pierceビオチン定量キットには、HABAとアビジンのプレミックスとビオチン標識された西洋わさびペルオキシダーゼ（HRP）陽性コントロールが含まれています。HABA-アビジンのプレミックスは、便利なThermo Scientific No-Weighマイクロチューブパッケージで提供され、少量の試薬を計量する難しさを解消します。

ビオチン化を定量化するには、ビオチン化タンパク質を含む溶液をHABAとアビジンの混合物に加えます。アビジンに対するビオチンの親和性が高いため、ビオチンはHABAに置き換わり、500 nmでの吸光度が比例的に減少します。この方法により、ビオチン含有サンプルの添加前後のHABA-アビジン溶液の吸光度を測定することで、溶液中に存在する未知の量のビオチンを単一のキュベットで定量できます。吸光度の変化は、HABA-アビジン複合体の吸光係数によるサンプル中のビオチンの量と関連があります。オンラインHABA計算ツールを見る。

研究用途にのみご使用ください。診断目的には使用できません。
仕様
製品ラインPierce
製品タイプBiotin Quantitation Kit
数量12 Tests
Labeling Target抗体, タンパク質
標識または色素ビオチン
Unit SizeEach
組成および保存条件
対応範囲：PEG12のタンパク質試料のビオチン化試験
• HABA-アビジンのプレミックス、24チューブ
• ビオチン標識された西洋ワサビペルオキシダーゼ、5 mg

4℃で保存

よくあるご質問（FAQ）

How can I determine the amount of biotinylation on my ligand?

An assay with HABA dye (Cat. No. 28010 or 28005) can be used to determine the molarity of biotin in solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you provide the shelf life for Pierce Biotin Quantitation Kit (28005)?

Pierce Biotin Quantitation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How can I determine the degree of protein labeling?

To quantitate biotin, we offer two kits as follows:

  • Biotin Quantitation Kit (Cat. No. 28005): With this kit, a solution containing the biotinylated protein is added to a mixture of HABA reagent (4’-hydroxyazobenzene-2-carboxylic acid and avidin. Because of its higher affinity for avidin, biotin displaces the HABA and the absorbance at 500nm decreases proportionally. 
  • Fluorescence Biotin Quantitation kit (Cat. No. 46610): This microplate-based biotin assay is easy to perform by adding the supplied fluorescent reporter to the biotinylated samples and diluted biocytin standards. The avidin fluoresces when the weakly interacting HABA (4’-hydroxyazobenzene-2-carboxylic acid) is displaced by the biotin. The amount of biotin is determined by comparing the sample's fluorescence to the biocytin standard curve. This assay requires must less sample volume than the microplate colorimetric HABA assay and is much more sensitive

    • To determine the dye-to-protein ratio after fluorophore conjugation, absorbance readings of the protein:dye conjugate are taken and the molar ratio can then be calculated. Please go to this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0031-Calc-FP-ratios.pdf")for more information

    Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

    引用および参考文献 (10)

    引用および参考文献
    Abstract
    Buoyancy-activated cell sorting using targeted biotinylated albumin microbubbles.
    Authors:Liou YR, Wang YH, Lee CY, Li PC
    Journal:
    PubMed ID:25993512
    'Cell analysis often requires the isolation of certain cell types. Various isolation methods have been applied to cell sorting, including fluorescence-activated cell sorting and magnetic-activated cell sorting. However, these conventional approaches involve exerting mechanical forces on the cells, thus risking cell damage. In this study we applied a novel isolation ... More
    A general protocol for the generation of Nanobodies for structural biology.
    Authors:Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J
    Journal:
    PubMed ID:24577359
    There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) proteins and complexes thereof. Our technology has a competitive ... More
    Development of a novel, guinea pig-specific IFN-? ELISPOT assay and characterization of guinea pig cytomegalovirus GP83-specific cellular immune responses following immunization with a modified vaccinia virus Ankara (MVA)-vectored GP83 vaccine.
    Authors:Gillis PA, Hernandez-Alvarado N, Gnanandarajah JS, Wussow F, Diamond DJ, Schleiss MR
    Journal:
    PubMed ID:24856783
    The guinea pig (Cavia porcellus) provides a useful animal model for studying the pathogenesis of many infectious diseases, and for preclinical evaluation of vaccines. However, guinea pig models are limited by the lack of immunological reagents required for characterization and quantification of antigen-specific T cell responses. To address this deficiency, ... More
    Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules.
    Authors:Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente-Muñoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, Mackereth CD, Sainlos M
    Journal:Nat Commun
    PubMed ID:31586061
    'Designing highly specific modulators of protein-protein interactions (PPIs) is especially challenging in the context of multiple paralogs and conserved interaction surfaces. In this case, direct generation of selective and competitive inhibitors is hindered by high similarity within the evolutionary-related protein interfaces. We report here a strategy that uses a semi-rational ... More
    A Monoclonal-Monoclonal Antibody Based Capture ELISA for Abrin.
    Authors:Tam CC, Cheng LW, He X, Merrill P, Hodge D, Stanker LH
    Journal:Toxins (Basel)
    PubMed ID:29057799
    Abrin, one of the most highly potent toxins in the world, is derived from the plant,
    View all Pierce™ Biotin Quantitation Kit citations