Buoyancy-activated cell sorting using targeted biotinylated albumin microbubbles.
AuthorsLiou YR, Wang YH, Lee CY, Li PC
Journal
PubMed ID25993512
'Cell analysis often requires the isolation of certain cell types. Various isolation methods have been applied to cell sorting, including fluorescence-activated cell sorting and magnetic-activated cell sorting. However, these conventional approaches involve exerting mechanical forces on the cells, thus risking cell damage. In this study we applied a novel isolation ... More
A general protocol for the generation of Nanobodies for structural biology.
AuthorsPardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J
Journal
PubMed ID24577359
There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) proteins and complexes thereof. Our technology has a competitive ... More
Development of a novel, guinea pig-specific IFN-? ELISPOT assay and characterization of guinea pig cytomegalovirus GP83-specific cellular immune responses following immunization with a modified vaccinia virus Ankara (MVA)-vectored GP83 vaccine.
The guinea pig (Cavia porcellus) provides a useful animal model for studying the pathogenesis of many infectious diseases, and for preclinical evaluation of vaccines. However, guinea pig models are limited by the lack of immunological reagents required for characterization and quantification of antigen-specific T cell responses. To address this deficiency, ... More
Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules.
AuthorsRimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente-Muñoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, Mackereth CD, Sainlos M
JournalNat Commun
PubMed ID31586061
'Designing highly specific modulators of protein-protein interactions (PPIs) is especially challenging in the context of multiple paralogs and conserved interaction surfaces. In this case, direct generation of selective and competitive inhibitors is hindered by high similarity within the evolutionary-related protein interfaces. We report here a strategy that uses a semi-rational ... More
A Monoclonal-Monoclonal Antibody Based Capture ELISA for Abrin.
Abrin, one of the most highly potent toxins in the world, is derived from the plant,
Influence of particle size and shape on their margination and wall-adhesion: implications in drug delivery vehicle design across nano-to-micro scale.
AuthorsCooley M, Sarode A, Hoore M, Fedosov DA, Mitragotri S, Sen Gupta A
JournalNanoscale
PubMed ID30080212
Intravascular drug delivery technologies majorly utilize spherical nanoparticles as carrier vehicles. Their targets are often at the blood vessel wall or in the tissue beyond the wall, such that vehicle localization towards the wall (margination) becomes a pre-requisite for their function. To this end, some studies have indicated that under ... More
Highly stable hexitol based XNA aptamers targeting the vascular endothelial growth factor.
AuthorsEremeeva E, Fikatas A, Margamuljana L, Abramov M, Schols D, Groaz E, Herdewijn P
JournalNucleic Acids Res
PubMed ID30968117
Biomedical applications of nucleic acid aptamers are limited by their rapid degradation in biological fluids and generally demand tedious post-selection modifications that might compromise binding. One possible solution to warrant biostability is to directly evolve chemically modified aptamers from xenobiotic nucleic acids (XNAs). We have isolated fully modified 2'-O-methyl-ribose-1,5-anhydrohexitol nucleic ... More
BIN1 favors the spreading of Tau via extracellular vesicles.
AuthorsCrotti A, Sait HR, McAvoy KM, Estrada K, Ergun A, Szak S, Marsh G, Jandreski L, Peterson M, Reynolds TL, Dalkilic-Liddle I, Cameron A, Cahir-McFarland E, Ransohoff RM
JournalSci Rep
PubMed ID31263146
Despite Bridging INtegrator 1 (BIN1) being the second most statistically-significant locus associated to Late Onset Alzheimer's Disease, its role in disease pathogenesis remains to be clarified. As reports suggest a link between BIN1, Tau and extracellular vesicles, we investigated whether BIN1 could affect Tau spreading via exosomes secretion. We observed ... More
Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System.
The variable lymphocyte receptors (VLRs) consist of leucine rich repeats (LRRs) and comprise the humoral antibodies produced by lampreys and hagfishes. The diversity of the molecules is generated by stepwise genomic rearrangements of LRR cassettes dispersed throughout the VLRB locus. Previously, target-specific monovalent VLRB antibodies were isolated from sea lamprey ... More
Modulation of virus-induced NF-?B signaling by NEMO coiled coil mimics.
AuthorsSadek J, Wuo MG, Rooklin D, Hauenstein A, Hong SH, Gautam A, Wu H, Zhang Y, Cesarman E, Arora PS
JournalNat Commun
PubMed ID32286300
Protein-protein interactions featuring intricate binding epitopes remain challenging targets for synthetic inhibitors. Interactions of NEMO, a scaffolding protein central to NF-?B signaling, exemplify this challenge. Various regulators are known to interact with different coiled coil regions of NEMO, but the topological complexity of this protein has limited inhibitor design. We ... More