Thermo Scientific™

      HABA (4'-hydroxyazobenzene-2-carboxylic acid)

      HABA (4'-hydroxyazobenzene-2-carboxylic acid)

      Catalog number:  28010

       Related applications: Protein Labeling & Crosslinking

        Contact us for support ›

      Error loading your content!

        Catalog number
      Select a plan
      Unit size
      		 Price ({{currency}}) Availability Qty
      {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
      		{{product.availability.message}} —
      Pro add-ons
      Customize this product ›

      Your on-site stock

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
      ›› {{supplyCenter.scName}}(Out of stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Change selected location › Order non-stocked items
      Back to top

      Description

      Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

      Features of HABA:

      • HABA-avidin complex can be used over a wide range of pH and salt concentrations
      • Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
      • Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions
      • Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)

      Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein. View online HABA Calculator.

      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Label or Dye: Biotin
      Labeling Target: Antibodies (General), Proteins (General)
      Product Line: Pierce™
      Product Size: 10 g

      Contents & storage

      Upon receipt store product at room temperature. Product shipped at ambient temperature.

      Documents

      Manuals & protocols

      Recommended products