Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeledRead more
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Catalog Number
Quantity
28010
10 g
Catalog number 28010
Price (USD)
171.00
Each
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Quantity:
10 g
Request bulk or custom format
Price (USD)
171.00
Each
Add to cart
Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.
Features of HABA:
• HABA-avidin complex can be used over a wide range of pH and salt concentrations • Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye • Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions • Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)
Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein. View online HABA Calculator.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LinePierce™
Product TypeHABA
Quantity10 g
Labeling TargetAntibodies, Proteins
Label or DyeBiotin
Unit SizeEach
Contents & Storage
Upon receipt store product at room temperature. Product shipped at ambient temperature.
Frequently asked questions (FAQs)
How can I determine the amount of biotinylation on my ligand?
An assay with HABA dye (Cat. No. 28010 or 28005) can be used to determine the molarity of biotin in solution.