Search
Invitrogen
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 99
Mouse IgG (H+L) Secondary Antibody (31430) in WB
Chemiluminiscent western blot was performed using Goat anti-Mouse IgG (Heavy Chain) Secondary Antibody, HRP (Product # 31430). Whole cell extracts of U-87 MG (Lane 1, 2, 3, 4), SH-SY5Y (Lane 5, 6, 7, 8), HeLa (Lane 9) and Hep G2 (Lane 10) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03221BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB23001) b... View More
Product Details
31430
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:200,000
Immunohistochemistry (Paraffin) (IHC (P))
1:500
ELISA (ELISA)
1:10,000-1:25,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Amount
1.6 mg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31430 has been validated for use in Western blot, immunohistochemistry (IHC), ELISA, and immunoprecipitation (IP) applications.
This product reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Storage Conditions
Store at 2-8°C prior to reconstitution.
Reconstitution and Storage Guidelines
Short-term storage (≤1 week): Reconstitute with 2 mL distilled water. Store at 2-8°C.
Long-term storage: Reconstitute with 1 mL distilled water, add 1 mL glycerol, and store at -20°C. Stable for up to 1 year.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support