Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays.
AuthorsRossotti MA, Pirez M, Gonzalez-Techera A, Cui Y, Bever CS, Lee KS, Morisseau C, Leizagoyen C, Gee S, Hammock BD, González-Sapienza G
Journal
PubMed ID26544909
Single domain heavychain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. However, there is no simple method to select the most valuable nanobodies from the crowd of positive clones obtained after the initial screening. In this paper, we describe a novel nanobody-based platform that ... More
Generation and characterization of antibodies against Asian elephant (Elephas maximus) IgG, IgM, and IgA.
AuthorsHumphreys AF, Tan J, Peng R, Benton SM, Qin X, Worley KC, Mikulski RL, Chow DC, Palzkill TG, Ling PD
Journal
PubMed ID25658336
Asian elephant (Elephas maximus) immunity is poorly characterized and understood. This gap in knowledge is particularly concerning as Asian elephants are an endangered species threatened by a newly discovered herpesvirus known as elephant endotheliotropic herpesvirus (EEHV), which is the leading cause of death for captive Asian elephants born after 1980 ... More
A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post-vaccination monitoring.
AuthorsWang L, Mi S, Madera R, Ganges L, Borca MV, Ren J, Cunningham C, Cino-Ozuna AG, Li H, Tu C, Gong W, Shi J
JournalBMC Vet Res
PubMed ID31937302
'Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention. ... More
A novel monoclonal antibodies-based sandwich ELISA for detection of serotype 4 fowl adenovirus.
AuthorsShao H, Wang P, Wang W, Zhang J, Li T, Liang G, Gao W, Qin A, Ye J
JournalAvian Pathol
PubMed ID30621493
'As a major causative agent for hepatitis-hydropericardium syndrome (HPS) in chickens, serotype 4 fowl adenovirus (FAdV-4) has caused huge economic losses in the poultry industry globally. However, there is no commercial diagnostic test for FAdV-4 antigens. To generate a rapid approach for specific detection of FAdV-4, a monoclonal antibodies (mAbs)-based ... More
A novel PSMA/GCPII-deficient mouse model shows enlarged seminal vesicles upon aging.
AuthorsVorlová B, Sedlák F, Kašpárek P, Šrámková K, Malý M, Zámecník J, Šácha P, Konvalinka J
JournalProstate
PubMed ID30256431
'Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is an important diagnostic and therapeutic target in prostate cancer. PSMA/GCPII is also expressed in many healthy tissues, but its function has only been established in the brain and small intestine. Several research groups have attempted to produce PSMA/GCPII-deficient ... More
RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence.
AuthorsLopez-Guerrero AM, Espinosa-Bermejo N, Sanchez-Lopez I, Macartney T, Pascual-Caro C, Orantos-Aguilera Y, Rodriguez-Ruiz L, Perez-Oliva AB, Mulero V, Pozo-Guisado E, Martin-Romero FJ
JournalSci Rep
PubMed ID32313105
'Tumor invasion requires efficient cell migration, which is achieved by the generation of persistent and polarized lamellipodia. The generation of lamellipodia is supported by actin dynamics at the leading edge where a complex of proteins known as the WAVE regulatory complex (WRC) promotes the required assembly of actin filaments to ... More
A rapid, on-bead enzyme-linked immunosorbent assay for
Predilection of Low Protein C-induced Spontaneous Atherothrombosis for the Right Coronary Sinus in Apolipoprotein E deficient mice.
AuthorsHeestermans M, Ouweneel AB, Hassan J, Kloosterman M, Reitsma PH, Gijbels MJJ, van Vlijmen BJM, van Eck M
JournalSci Rep
PubMed ID30305662
Silencing of anticoagulant protein C using RNA interference (siProc) evokes low incident but spontaneous atherothrombosis in the aortic root of apolipoprotein E-deficient (Apoe
Development and Evaluation of Epitope-Blocking ELISA for Detection of Antibodies against Contagious Caprine Pleuropneumonia in Goat Sera.
AuthorsJean de Dieu B, Charles BS, Nwankpa N, Chitsungo E, Moustapha Boukary CR, Maina N, Tefera TA, Nwankpa RV, Mwangi N, Mathurin Koffi Y
JournalVet Sci
PubMed ID31635322
Enzyme linked immunosorbent assays (ELISAs) have been developed for the detection of antibodies against contagious caprine pleuropneumonia (CCPP), the causative agent of which is
The unconventional biogenesis of Kv7.1-KCNE1 complexes.
AuthorsOliveras A, Serrano-Novillo C, Moreno C, de la Cruz A, Valenzuela C, Soeller C, Comes N, Felipe A
JournalSci Adv
PubMed ID32270035
The potassium channel Kv7.1 associates with the KCNE1 regulatory subunit to trigger cardiac
Internalization mechanisms of brain-derived tau oligomers from patients with Alzheimer's disease, progressive supranuclear palsy and dementia with Lewy bodies.
AuthorsPuangmalai N, Bhatt N, Montalbano M, Sengupta U, Gaikwad S, Ventura F, McAllen S, Ellsworth A, Garcia S, Kayed R
JournalCell Death Dis
PubMed ID32366836
Tau aggregates propagate in brain cells and transmit to neighboring cells as well as anatomically connected brain regions by prion-like mechanisms. Soluble tau aggregates (tau oligomers) are the most toxic species that initiate neurodegeneration in tauopathies, such as Alzheimer's disease (AD), progressive supranuclear palsy (PSP), and dementia with Lewy bodies ... More
Keratinocyte transglutaminase 2 promotes CCR6
AuthorsShin JW, Kwon MA, Hwang J, Lee SJ, Lee JH, Kim HJ, Lee KB, Lee SJ, Jeong EM, Chung JH, Kim IG
JournalCell Death Dis
PubMed ID32355189
Keratinocyte-derived cytokines and chemokines amplify psoriatic inflammation by recruiting IL-17-producing CCR6
Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for porcine hemoglobin quantification.
AuthorsJiang X, Wu M, Dong W, Rao Q, Huo H, Han Q
JournalFood Chem
PubMed ID32344349
The major objective of this study was to establish a monoclonal antibody (mAb)-based sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of porcine hemoglobin (P
ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer patients undergoing therapy with trastuzumab.
AuthorsAgnolon V, Contato A, Meneghello A, Tagliabue E, Toffoli G, Gion M, Polo F, Fabricio ASC
JournalSci Rep
PubMed ID32080226
Circulating HER2 extracellular domain (HER2 ECD) levels were proposed as a surrogate for HER2 tissue expression to monitor breast cancer patients for early relapse or responses to standard or HER2-targeted therapies, such as the monoclonal antibody (mAb) trastuzumab. Currently, available commercial ELISA assays for HER2 ECD rely on antibodies recognizing undisclosed ... More
Exploiting species specificity to understand the tropism of a human-specific toxin.
AuthorsBoguslawski KM, McKeown AN, Day CJ, Lacey KA, Tam K, Vozhilla N, Kim SY, Jennings MP, Koralov SB, Elde NC, Torres VJ
JournalSci Adv
PubMed ID32195339
Many pathogens produce virulence factors that are specific toward their natural host. Clinically relevant methicillin-resistant