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Invitrogen
p38 MAPK Monoclonal Antibody (p38-3F11)
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FIGURE: 1 / 8
p38 MAPK Antibody (33-1300) in ICC/IF
Immunofluorescent analysis of p38-alpha (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a mouse p38 monoclonal antibody (Product # 33-1300) at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
33-1300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
p38-3F11
Immunogen
Full-length recombinant mouse p38 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
33-1300 has been successfully used in immunofluorescence, and western blot with human recombinant proteins and whole cell lysates of hamster, mouse, and primate origins.
33-1300 specifically recognizes both alpha and beta isoforms of p38, with slight reactivity to p38-delta.
Target Information
The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine-supressive anti-inflammatory drug binding protein; encoded by transcript variant 4; p38 MAP kinase; encoded by transcript variant 5; p38 MAP kinase; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; SAPK2a; CSAIDS binding protein 1; stress-activated protein kinase 2A; unnamed protein product
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; Mxi2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539, (Mouse) P47811
Entrez Gene ID: (Human) 1432, (Mouse) 26416, (Rat) 81649
MAPK cascade
negative regulation of inflammatory response to antigenic stimulus
regulation of transcription from RNA polymerase II promoter
chemotaxis
signal transduction
cell surface receptor signaling pathway
positive regulation of gene expression
positive regulation of myotube differentiation
platelet activation
osteoclast differentiation
stress-activated protein kinase signaling cascade
positive regulation of cyclase activity
positive regulation of interleukin-12 production
negative regulation of hippo signaling
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
p38MAPK cascade
signal transduction in response to DNA damage
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
positive regulation of myoblast differentiation
vascular endothelial growth factor receptor signaling pathway
positive regulation of muscle cell differentiation
stress-activated MAPK cascade
3'-UTR-mediated mRNA stabilization
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to ionizing radiation
cellular response to UV-B
cellular senescence
stress-induced premature senescence
cellular response to virus
regulation of cytokine production involved in inflammatory response
positive regulation of myoblast fusion
positive regulation of reactive oxygen species metabolic process
DNA damage checkpoint
cell morphogenesis
cartilage condensation
angiogenesis
osteoblast differentiation
placenta development
response to dietary excess
chondrocyte differentiation
glucose metabolic process
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
transmembrane receptor protein serine/threonine kinase signaling pathway
skeletal muscle tissue development
myoblast differentiation involved in skeletal muscle regeneration
peptidyl-serine phosphorylation
fatty acid oxidation
regulation of ossification
lipopolysaccharide-mediated signaling pathway
response to muramyl dipeptide
response to lipopolysaccharide
response to insulin
tumor necrosis factor-mediated signaling pathway
response to muscle stretch
positive regulation of protein import into nucleus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
protein autophosphorylation
striated muscle cell differentiation
protein maturation
regulation of cardiac muscle cell proliferation
positive regulation of cardiac muscle cell proliferation
bone development
pyroptosis
cellular response to tumor necrosis factor
negative regulation of canonical Wnt signaling pathway
positive regulation of brown fat cell differentiation
regulation of synaptic membrane adhesion
NLRP1 inflammasome complex assembly
response to glucose
phosphorylation
cellular response to transforming growth factor beta stimulus
cellular response to acetaldehyde
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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