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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, Functional Grade, eBioscience™
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FIGURE: 1 / 98
MHC Class II (I-A/I-E) Antibody (36-5321-85) in Flow
Staining of C57BL/6 splenocytes with 0.015 µg of Rat IgG2b K Isotype Control Functional Grade Biotin (cat. 36-4031) (open histogram), or 0.015 µg of Anti-Mouse MHC Class II (I-A/I-E) Functional Grade Biotin followed by Streptavidin PE (cat. 12-4317) (filled histogram). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
36-5321-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M5/114.15.2
Conjugate
Biotin , Functional Grade
Biotin , Functional Grade
Biotin , Functional Grade
Form
Liquid
Concentration
0.5 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description
The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Applications Tested
Tested
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cell surface glycoprotein; class II Ia antigen A-beta; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H2-I-E-beta; H2-IA-beta cell surface glycoprotein; histocompatibility 2, class II antigen A, alpha, promoter 1; histocompatibility 2, class II antigen A, beta, promoter 1; histocompatibility 2, class II antigen E beta, promoter 1; I-Ab; I-Ad; I-Aq; I-Ar MHC class II molecule alpha chain; I-Ar MHC class II molecule beta chain; I-E beta MHC class II; I-Ed; I-Ek; IA-alpha polyprotein; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen alpha chain; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC E-beta-b precursor; MHC H2-I-A-beta chain; MHC H2-IA-alpha; MHC H2-IE-beta; MHC H2-IE-beta cell surface glycoprotein; MHC NOD beta chain precursor; MHC-II I-A(d) alpha; MHC-II I-A(d) beta; response to metastatic cancers 1; unnamed protein product
Gene Aliases: Aalpha; Abeta; Eb; H-2Aa; H-2Ab; H-2Eb; H2-Ab; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1
UniProt ID: (Mouse) P14434, (Mouse) P14483
Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969
immune system process
peptide antigen assembly with MHC class II protein complex
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of immune response
positive regulation of T cell activation
negative regulation of T cell proliferation
positive regulation of T cell differentiation
antigen processing and presentation of peptide antigen
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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