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Invitrogen
WNT1 Polyclonal Antibody
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FIGURE: 1 / 5
WNT1 Antibody (36-5800) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), A431 (Lane 2), SK-OV-3 (Lane 3), Jurkat (Lane 4), Hep G2 (Lane 5), NTERA-2 (Lane 6) and COS-7 (Lane7). The blots were probed with Anti-WNT1 Rabbit Polyclonal Antibody (Product # 36-5800, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 41 kDa band corresponding to WNT1 was observed across cell lines tested. Known quantity of protein samples were electrophore... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
36-5800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the human WNT1 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: proto-oncogene Int-1; proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; unnamed protein product; wingless-related MMTV integration site 1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)
Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1
UniProt ID: (Human) P04628, (Mouse) P04426
Entrez Gene ID: (Human) 7471, (Rhesus monkey) 709009, (Mouse) 22408
embryonic axis specification
cell-cell signaling
positive regulation of cell proliferation
response to wounding
positive regulation of lamellipodium assembly
negative regulation of cell-substrate adhesion
Wnt signaling pathway
cerebellum development
central nervous system morphogenesis
cerebellum formation
neurogenesis
negative regulation of cell-cell adhesion
neuron differentiation
negative regulation of BMP signaling pathway
midbrain development
cell proliferation in midbrain
astrocyte-dopaminergic neuron signaling
signal transduction in response to DNA damage
negative regulation of apoptotic process
positive regulation of insulin-like growth factor receptor signaling pathway
cell fate commitment
negative regulation of cell differentiation
negative regulation of fat cell differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of fibroblast proliferation
Spemann organizer formation
canonical Wnt signaling pathway
bone development
positive regulation of dermatome development
dopaminergic neuron differentiation
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
midbrain dopaminergic neuron differentiation
embryonic brain development
negative regulation of cellular senescence
branching involved in ureteric bud morphogenesis
positive regulation of protein phosphorylation
regulation of transcription, DNA-templated
signal transduction
myoblast fusion
organ morphogenesis
myotube differentiation
spinal cord association neuron differentiation
diencephalon development
forebrain anterior/posterior pattern specification
midbrain-hindbrain boundary maturation during brain development
metencephalon development
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
midbrain-hindbrain boundary development
organ regeneration
T cell differentiation in thymus
inner ear morphogenesis
positive regulation of transcription from RNA polymerase II promoter
neuron fate commitment
neuron fate determination
hepatocyte differentiation
cellular response to peptide hormone stimulus
hematopoietic stem cell proliferation
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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