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Invitrogen
c-Met Monoclonal Antibody (3D4)
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Product Details
37-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3D4
Immunogen
Synthetic peptide derived from cytoplasmic domain of human c-Met protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the cytoplasmic domain of the c-Met protein. On western blotting, this antibody recognizes a band at approximately 145 kDa. During development reactivity was confirmed with human HT-29 breast carcinoma lysates and c-Met cytoplasmic domain recombinant protein by both western blot, and with formalin-fixed, paraffin-embedded breast carcinoma tissues by immunohistochemistry.
For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, heat-induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Recommended antibody incubation period is overnight at 4°C.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met oncogene; MET proto-oncogene protein; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; tyrosine kinase; hepatocyte growth factor receptor; HGFR; Tyrosine-protein kinase Met; unnamed protein product
Gene Aliases: AUTS9; c-Met; DA11; DFNB97; HGFR; MET; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
kinase activity
transferase activity
semaphorin receptor activity
protein phosphatase binding
identical protein binding
molecular function activator activity
endothelial cell morphogenesis
liver development
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of gene expression
negative regulation of autophagy
neuron differentiation
pancreas development
positive regulation of microtubule polymerization
negative regulation of Rho protein signal transduction
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
cell development
animal organ development
branching morphogenesis of an epithelial tube
positive chemotaxis
negative regulation of stress fiber assembly
excitatory postsynaptic potential
establishment of skin barrier
negative regulation of thrombin receptor signaling pathway
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of guanyl-nucleotide exchange factor activity
positive regulation of endothelial cell chemotaxis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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